Abstract
Extracellular enzyme activities of laccase and cellulase and their transcriptional regulation were investigated at various growth stages in a sawdust-based substrate forLentinula edodes. Changes of laccase and cellulase activities revealed a clear relationship with fruit body development stages. Laccase and cellulase activities were regulated at the level of gene transcription. The level of laccase mRNA was maximal at the fully colonized stage and declined during fruit body development. Cellulase mRNA began to accumulate at the pin (miniature fruit bodies) formation stage. Cellulase mRNA transcripts were maximally expressed at the veil-break stage of fruit body development. This tendency was clearer in the fruiting cultures with the wide-range-weather strains than in non-fruiting cold-weather strains. Transcription of laccase and cellulase genes was also affected by the water conditions of the sawdust-based substrate. Primordia initiation occurred when the water potential of the medium was high for rapid mRNA transcription by the mycelium.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Literature cited
Chow, C-M., Yague, E., Raguz, S., Wood, D. A. and Thurston, C. F. 1994. Thece/3 gene ofAgaricus bisporus codes for a modular cellulase and is transcriptionally regulated by the carbon source. Appl. Environ. Microbiol.60: 2779–2785.
Claydon, N., Allan, M. and Wood, D. A. (1988) Fruit body biomass regulated production of extracellular endocellulase during periodic fruiting byAgaricus bisporus. Trans. Br. Mycol. Soc.90: 85–90.
Collins, P. J. and Dobson, A. D. W. 1997. Regulation of lacase gene trancription inTrametes versicolor. Appl. Environ. Microbiol.63: 3444–3450.
Diehle, D. and Royse, D. J. 1986. Shiitake cultivation on sawdust: evaluation of selected genotypes for biological efficiency and mushroom size. Mycologia78: 929–933.
Gettemy, J. M., Ma, B., Alic, M. and Gold, M. H. 1998. Reverse transcription-PCR analysis of the regulation of the manganese peroxidase gene family. Appl. Environ. Microbiol.64: 569–574.
Leatham, G. F. and Stahmann, M. A. 1981. Studies on the laccase ofLentinus edodes: Specificity, localization and association with the development of fruiting bodies. J. Gen. Microbiol.125: 147–157.
Leatham, G. F. and Stahmann, M. A. 1984. Stimulatory effect of nickel or tin on fruiting ofLentinus edodes. Trans. Br. Mycol. Soc.83: 513–517.
Leatham, G. F. 1985. Extracellular enzymes produced by the cultivated mushroomLentinus edodes during degradation of a lignocellulosic medium. Appl. Environ. Microbiol.50: 859–867.
Manning, K. and Wood, D. A. 1983. Production and regulation of cellulase ofAgaricus bisporus. J. Gen. Microbiol.129: 1839–1847.
Ohga, S. 1992. Comparison of extracellular enzyme activities among different strains ofLentinus edodes grown on sawdust-based cultures in relationship to their fruiting abilities. J. Japan Wood Res. Soc.38: 310–316.
Ohga, S., Roozendael, F. V., Aspinwall, M. and Miwa, M. 1992. Yield and size response of the shiitake mushroom,Lentinus edodes, depending on incubation time on sawdust-based culture. Trans. Mycol. Soc. Japan33: 349–357.
Ohga, S. 1995. Sawdust-based cultivation ofLentinula edodes and degree of culture maturity. Mushroom Sci. Biotechnol.2: 1–13 (In Japanese.)
Ohga, S. 1998. The influence of genotype on the production ofLentinula edodes grown on sawn on sawdust-based substrate. J. Fac. Agr., Kyushu Univ.43: 39–45.
Ohga, S. 1999. Effect of water potential on fruit body formation ofLentinula edodes in sawdust-based substrate. J. Wood Sci.45: 337–342.
Ohga, S., Smith, M., Thurston, C. F. and Wood, D. A. 1999. Transcription regulation of laccase and cellulase genes in the mycelium ofAgaricus bisporus during fruit body development on a solid substrate. Mycol. Res.103: 1557–1560.
Perry, C. R., Smith, M., Britnell, C. H., Wood, D. A. and Thurston, C. F. 1993. Identification of two laccase genes in the cultivated mushroomAgaricus bisporus. J. Gen. Microbiol.139: 1209–1218.
Raguz, S., Yague, E., Wood, D. A. and Thurston, C. F. 1992. Isolation and characterization of a cellulose-growth-specific gene fromAgaricus bisporus. J. Gen. Microbiol.139: 1209–1218.
Royse, D. J. 1985. Effect of spawn run time and substrate nutrient on yield and size of shiitake mushroom. Mycologia77: 756–762.
Royse, D. J. and Bahler, C. C. 1986. Effects of genotype, spawn run time, and substrate formation on biological efficiency of shiitake. Appl. Environ. Microbiol.52: 1425–1427.
Smith, M., Shnyreva, A., Wood, D. A. and Thurston, C. F. 1998. Tandem organization and highly disparate expression of the two laccase geneslcc1 andlcc2 in the cultivated mushroomAgaricus bisporus. Microbiology144: 1063–1069.
Stoop, J. M. H. and Mooibroek, H. 1998. Cloning and characterization of NADP-mannitol dehydrogenase cDNA from the button mushroom,Agaricus bisporus, and its expression in response to NaCl stress. Appl. Environ. Microbiol.64: 4689–4696.
Turner, E. M. 1974. Phenoloxidase activity in relation to substrate and development stage in the mushroomAgaricus bisporus. Trans. Br. Mycol. Soc.63: 541–547.
Turner, E. M., Wright, M., Ward, T., Osborne D. J. and Self, R. 1975. Production of ethylene and other volatiles and changes in cellulase and laccase activities during the life cycle of the cultivated mushroomAgaricus bisporus. J. Gen. Miclobiol.91: 167–176.
Vallim, M. A., Janse, B. J. H., Gaskell, J., Pizzirani-Kleiner, A. A. and Cullen, D. 1998.Phanerochaete chrysosporium cellobiohydrolase and cellobiose dehydrogenase transcripts in wood. Appl. Environ. Microbiol.64: 1924–1928.
Wood, D. A. and Goodenough, P. W. 1977. Fruiting ofAgaricus bisporus: changes in extracellular enzyme activities during growth and fruiting. Arch. Microbiol.114: 161–165.
Yague, E., Wood, D. A. and Thurston, C. F. 1994. Regulation of transcription of thecel1 gene inAgaricus bisporus. Mol. Microbiol.12: 41–47.
Yague, E., Chow, C-M., Challen, M. and Thurston, C. F. 1996. Correlation of exons with functional domains and folding regions in a cellulase fromAgaricus bisporus. Curr. Genet.30: 56–61.
Yague, E., Mehak-Zunic, M., Morgan, L., Wood, D. A. and Thurston, C. F. 1997. Expression ofcel2 andcel4 proteins fromAgaricus bisporus, with similarity to fungal cellobiohydrolase 1 and β-mannanase, respectively, is regulated by the carbon source. Microbiology143: 239–244.
Author information
Authors and Affiliations
Corresponding author
About this article
Cite this article
Ohga, S., Cho, NS., Thurston, C.F. et al. Transcriptional regulation of laccase and cellulase in relation to fruit body formation in the mycelium ofLentinula edodes on a sawdust-based substrate. Mycoscience 41, 149–153 (2000). https://doi.org/10.1007/BF02464324
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02464324