Skip to main content
SpringerLink
Log in

In situ recovery of 2,3-butanediol from fermentation by liquid–liquid extraction

  • Original Paper
  • Published:
Journal of Industrial Microbiology & Biotechnology

An Erratum to this article was published on 19 May 2009

Abstract

End-product conversion, low product concentration and large volumes of fermentation broth, the requirements for large bioreactors, in addition to the high cost involved in generating the steam required to distil fermentation products from the broth largely contributed to the decline in fermentative products. These considerations have motivated the study of organic extractants as a means to remove the product during fermentation and minimize downstream recovery. The aim of this study is to assess the practical applicability of liquid–liquid extraction in 2,3-butanediol fermentations. Eighteen organic solvents were screened to determine their biocompatibility, and bioavailability for their effects on Klebsiella pneumoniae growth. Candidate solvents at first were screened in shake flasks for toxicity to K. pneumoniae. Cell density and substrate consumption were used as measures of cell toxicity. The possibility of employing oleyl alcohol as an extraction solvent to enhance end product in 2,3-butanediol fermentation was evaluated. Fermentation was carried out at an initial glucose concentration of 80 g/l. Oleyl alcohol did not inhibit the growth of the fermentative organism. 2,3-Butanediol production increased from 17.9 g/l (in conventional fermentation) to 23.01 g/l (in extractive fermentation). Applying oleyl alcohol as the extraction solvent, about 68% of the total 2,3-butanediol produced was extracted.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

References

  1. Shi Z, Zhang C, Chen J, Mao Z (2005) Performance evaluation of acetone–butanol continuous flash extractive fermentation process. Bioprocess Biosyst Eng 27:175–183. doi:10.1007/s00449-004-0396-7

    Article  PubMed  CAS  Google Scholar 

  2. Cheng HC, Wang FS (2007) Trade-off optimal design of a biocompatible solvent for an extractive fermentation process. Chem Eng Sci 62:4316–4324. doi:10.1016/j.ces.2007.05.010

    Article  CAS  Google Scholar 

  3. Martik J, Rosenberg M, Schlosser S, Kristofikovi C (1995) Toxicity of organic solvents used in situ microbial fermentation. Biotechnol Tech 9(4):247–252. doi:10.1007/BF00151570

    Article  Google Scholar 

  4. Serrano-Carreón L, Balderas-Ruíz K, Galindo E, Rito-Palomares M (2002) Production and biotransformation of 6-pentyl-α-pyrone by Trichoderma harzianumin two-phase culture systems. Appl Microbiol Biotechnol 58:170–174. doi:10.1007/s00253-001-0874-7

    Article  PubMed  Google Scholar 

  5. Eiteman MA, Gainer JL (1989) In situ extraction versus the use of an external column in fermentation. Appl Microbiol Biotechnol 30:614–618. doi:10.1007/BF00255368

    Article  CAS  Google Scholar 

  6. Ghosh S, Swaminathan T (2003) Optimization of process variables for the extractive fermentation of 2,3-butanediol by Klebsiella oxytoca in aqueous two-phase system using response surface methodology. Chem Biochem Eng Q 17(4):319–325

    Google Scholar 

  7. Janikowski TB, Velicogna D, Punt M, Daugulis AJ (2002) Use of a two-phase partitioning bioreactor for degrading polycyclic aromatic hydrocarbons by a Sphingomonas sp. Appl Microbiol Biotechnol 59:368–376. doi:10.1007/s00253-002-1011-y

    Article  PubMed  CAS  Google Scholar 

  8. Ghanadzadeh Gilani H, Khayati G, Haghi AH (2006) Liquid–liquid equilibria of (water + 2, 3-butanediol + 2-ethyl-1-hexanol) at several temperatures. Fluid Phase Equil 247:199–204. doi:10.1016/j.fluid.2006.06.025

    Article  Google Scholar 

  9. Heipieper HJ, Neumann G, Cornelissen S, Meinhardt F (2007) Solvent-tolerant bacteria for biotransformation in two-phase fermentation systems. Appl Microbiol Biotechnol 74:961–973. doi:10.1007/s00253-006-0833-4

    Article  PubMed  CAS  Google Scholar 

  10. MacLeod CT, Daugulis AJ (2003) Biodegradation of polycyclic aromatic hydrocarbons in a two-phase partitioning bioreactor in the presence of a bioavailable solvent. Appl Microbiol Biotechnol 62:291–296. doi:10.1007/s00253-003-1297-4

    Article  PubMed  CAS  Google Scholar 

  11. Pudge IB, Daugulis AJ, Dubois C (2003) The use of Enterobacter cloacae ATCC 43560 in the development of a two-phase partitioning bioreactor for the destruction of hexahydro-1,3,5-trinitro-1,3,5-s-triazine (RDX). J Biotechnol 100:65–75. doi:10.1016/S0168-1656(02)00229-8

    Article  PubMed  CAS  Google Scholar 

  12. Aono R, Tsukagoshi N, Miyamoto T (2001) Evaluation of the growth inhibition strength of hydrocarbon solvents against Escherichia coli and Pseudomonas putida grown in a two-liquid phase culture system consisting of a medium and organic solvent. Extremophiles 5:11–15. doi:10.1007/s007920000167

    Article  PubMed  CAS  Google Scholar 

  13. Lotter J, Botes AL, Van Dyk MS, Breytenbach J (2004) Correlation between the physicochemical properties of organic solvents and their biocompatibility toward epoxide hydrolase activity in whole-cells of a yeast, Rhodotorula sp. Biotechnol Lett 26:1191–1195. doi:10.1023/B:BILE.0000036595.09808.c2

    Article  PubMed  CAS  Google Scholar 

  14. Collins LD, Daugulis AJ (1999) Benzene/toluene/p-xylene degradation. Part I. Solvent selection and toluene degradation in a two-phase partitioning bioreactor. Appl Microbiol Biotechnol 52:354–359. doi:10.1007/s002530051531

    Article  PubMed  CAS  Google Scholar 

  15. Zigova′ J, Sturdı’k E, Vanda’k D, Schlosser S (1999) Butyric acid production by Clostridium butyricum with integrated extraction and pertraction. Process Biochem 34:835–843. doi:10.1016/S0032-9592(99)00007-2

    Article  Google Scholar 

  16. Qin J, Xiao Z, Ma C, Xie N, Liu P, Xu P (2006) Production of 2,3-Butanediol by Klebsiella oxytoca using glucose and ammonium phosphate. Chin J Chem Eng 14(1):132–136. doi:10.1016/S1004-9541(06)60050-5

    Article  CAS  Google Scholar 

  17. Syu MJ (2001) Biological production of 2,3-butanediol. Appl Microbiol Biotechnol 55:10–18. doi:10.1007/s002530000486

    Article  PubMed  CAS  Google Scholar 

  18. Yu EKC, Saddler JN (1983) Fed-batch approach to production of 2, 3-butanediol by Klebsiella pneumoniae grown on high substrate concentrations. Appl Environ Microbiol 46(3):630–635

    PubMed  CAS  Google Scholar 

  19. Ishizaki A, Michiwaki S, Crabbe F, Kobayashi G, Sonomoto K, Yoshino S (1999) Extractive acetone–butanol–ethanol fermentation using methylated crude palm oil as extractant in batch culture of Clostridium saccharoperbutylacetonicum Nl-4 (ATCC 13564). J Biosci Bioeng 87(3):352–356. doi:10.1016/S1389-1723(99)80044-9

    Article  PubMed  CAS  Google Scholar 

  20. Wang Y, Achenie LEK (2002) Computer aided solvent design for extractive fermentation. Fluid Phase Equil 201:1–18. doi:10.1016/S0378-3812(02)00073-0

    Article  CAS  Google Scholar 

  21. Porto TS, Monyeiro TIR, Moreira KA, Lima-Filho JL, Silva MPC, Porto ALF (2005) Liquid–liquid extraction of an extracellular alkaline protease from fermentation broth using aqueous two-phase and reversed micelles systems. World J Microbiol Biotechnol 21:655–659. doi:10.1007/s11274-004-3570-9

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Biology, Islamic Azad University, Rasht branch, Rasht, Iran

    Masumeh Anvari

  2. Department of Chemical Engineering, Engineering Faculty, Guilan University, Rasht, Iran

    Gholam Khayati

Authors
  1. Masumeh Anvari
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Gholam Khayati
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Masumeh Anvari.

Additional information

An erratum to this article can be found at http://dx.doi.org/10.1007/s10295-009-0586-z

Rights and permissions

Reprints and permissions

About this article

Cite this article

Anvari, M., Khayati, G. In situ recovery of 2,3-butanediol from fermentation by liquid–liquid extraction. J Ind Microbiol Biotechnol 36, 313–317 (2009). https://doi.org/10.1007/s10295-008-0501-z

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10295-008-0501-z

Keywords

Search

Navigation