Skip to main content
SpringerLink
Log in

Effects of industrial storage on the bioreduction capacity of brewer’s yeast

  • Original Paper
  • Published:
Journal of Industrial Microbiology & Biotechnology

Abstract

The effects of industrial storage on the changes of the cell viability and the activities of intracellular alcohol dehydrogenase (ADH) and glucose-6-phosphate dehydrogenase (G6PDH) in brewer’s yeast, and the corresponding capacity for the bioconversion of ethyl-3-oxobutanoate (EOB) to ethyl (S)-3-hydroxybutanoate ((S)-EHB), were investigated. The viability of fresh brewer’s yeast cells stored in industrial circulating cooling water at 1–2°C showed 4 and 15% drop after the storage of 7 and 15 days, respectively, after which cells died rapidly. The pretreatment of the stored brewer’s yeast cells by washing and screening significantly enhanced cell viability during industrial storage. The intracellular levels of ADH and G6PDH after permeabilization of these stored cells with cetyltrimetylammonium bromide (CTAB) were much higher, which showed only slight decrease within 2 weeks during the industrial storage. When the stored cells after the permeabilization treatment was used as the biocatalyst at 90–120 g/L, EOB was converted almost completely into enantiopure (S)-EHB with an enantiomeric excess (ee) more than 99% and a yield of over 96%, by fed-batch bioconversion of 560 mM EOB within 6 h.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7
Fig. 8
Fig. 9

Similar content being viewed by others

References

  1. Rosen TC, Daussmann T, Stohrer J (2004) Bioreduction forms optically active 3-hydroxyesters. Spec Chem Mag 24:39–40

    Google Scholar 

  2. Albuquerque PM, Witt MA, Stambuk BU, Nascimento MG (2007) Influence of sugars on enantioselective reduction using Saccharomyces cerevisiae in organic solvent. Process Biochem 42(2):141–147. doi:10.1016/j.procbio.2006.07.034

    Article  CAS  Google Scholar 

  3. Kanda T, Miyata N, Fukui T, Kawamoto T, Tanaka A (1998) Doubly entrapped baker’s yeast survives during the long-term stereoselective reduction of ethyl 3-oxobutanoate in an organic solvent. Appl Microbiol Biotechnol 49:377–381. doi:10.1007/s002530051185

    Article  PubMed  CAS  Google Scholar 

  4. Chin-Joe I, Nelisse PM, Straathof AJJ, Jongejan JA, Pronk JT, Heijnen JJ (2000) Hydrolytic activity in baker’s yeast limits the yield of asymmetric 3-oxo ester reduction. Biotechnol Bioeng 69:370–376. doi:10.1002/1097-0290(20000820)69:4<370::AID-BIT3>3.0.CO;2-B

    Article  PubMed  CAS  Google Scholar 

  5. Chin-Joe I, Straathof AJJ, Pronk JT, Jongejan JA, Heijnen JJ (2002) Effect of high product concentration in a dual fed-batch asymmetric 3-oxo ester reduction by baker’s yeast. Biocatal Biotransformation 20:337–345. doi:10.1080/10242420290031370

    Article  CAS  Google Scholar 

  6. Buque EM, Chin-Joe I, Straathof AJJ, Jongejan JA, Heijnen JJ (2002) Immobilization affects the rate and enantioselectivity of 3-oxo ester reduction by baker’s yeast. Enzyme Microb Technol 31:656–664. doi:10.1016/S0141-0229(02)00161-8

    Article  CAS  Google Scholar 

  7. Chin-Joe I, Haberland J, Straathof AJJ, Jongejan JA, Liese A, Heijnen JJ (2002) Reduction of ethyl 3-oxobutanoate using non-growing baker’s yeast in a continuously operated reactor with cell retention. Enzyme Microb Technol 31(5):665–672. doi:10.1016/S0141-0229(02)00165-5

    Article  CAS  Google Scholar 

  8. Bonora GM, Drioli S, Forzato C, Nitti P, Pitacco G (2005) Baker’s yeast reduction of PEG-linked acetoacetate. Lett Org Chem 2:89–91. doi:10.2174/1570178053400135

    Article  CAS  Google Scholar 

  9. Carnell AJ, Head R, Bassett D, Schneider M (2004) Efficient large scale stereoinversion of (R)-ethyl 3-hydroxybutyrate. Tetrahedr Asym 15(5):821–825. doi:10.1016/j.tetasy.2003.12.005

    Article  CAS  Google Scholar 

  10. Johanson T, Katz M, Gorwa-Grauslund MF (2005) Strain engineering for stereoselective bioreduction of dicarbonyl compounds by yeast reductases. FEMS Yeast Res 5(6–7):513–525. doi:10.1016/j.femsyr.2004.12.006

    Article  PubMed  CAS  Google Scholar 

  11. Zhang J, Witholt B, Li Z (2006) Coupling of permeabilized microorganisms for efficient enantioselective reduction of ketone with cofactor recycling. Chem Commun 4:398–400

    Article  Google Scholar 

  12. Zhang J, Witholt B, Li Z (2006) Efficient NADPH Recycling in enantioselective bioreduction of a ketone with permeabilized cells of a microorganism containing a ketoreductase and a glucose 6-phosphate dehydrogenase. Adv Synth Catal 348:429–433. doi:10.1002/adsc.200505439

    Article  CAS  Google Scholar 

  13. Yu M-A, Wei Y-M, Jiang L, Xiao-bing Z, Qi W (2007) Bioconversion of ethyl 4-chloro-3-oxobutanoate to ethyl(R)-4-chloro-3- hydroxybutanoate by a permeabilized fresh brewer’s yeast cells in the presence of allyl bromide. J Ind Microbiol Biotechnol 34(2):151–156. doi:10.1007/s10295-006-0179-z

    Article  PubMed  CAS  Google Scholar 

  14. Powell CD, Van Zandycke SM, Quain DE, Smart KA (2000) Replicative ageing and scenescence in Saccharomyces cerevisiae and the impact on brewing. Ferm Microbiol 146:1023–1034

    CAS  Google Scholar 

  15. Gowda LR, Joshi MS, Bhat SG (1988) In situ assay of intracellular enzyme of yeast (Kluyveromyces tragilis) by digitonin permeabilization of cell membrane. Anal Biochem 175:531–536. doi:10.1016/0003-2697(88)90579-9

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgments

This work was supported by the special fund of three items of expenditure on science and technology department of Central District in ChongQing. We also thank the Chongqing Medical University for partial financial support of this work.

Author information

Authors and Affiliations

  1. Chongqing Key Laboratory of Biochemistry and Molecular Pharmacology, College of Pharmaceutical Sciences, Chongqing Medical University, Chongqing, 400016, China

    Ming-An Yu, Yi Hou, Geng-Hao Gong, Quan Zhao, Xiao-lan Yang & Fei Liao

  2. Chongqing Daxin Pharmaceutical Company Limited, Chongqing, 400700, China

    Xiao-bing Zhu

  3. College of Enviromental and Biological Engineering, Chongqing University of Technology and Business, Chongqing, 400067, China

    Lan Jiang

Authors
  1. Ming-An Yu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Yi Hou
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Geng-Hao Gong
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Quan Zhao
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Xiao-bing Zhu
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Lan Jiang
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Xiao-lan Yang
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Fei Liao
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Ming-An Yu.

Electronic supplementary material

Rights and permissions

Reprints and permissions

About this article

Cite this article

Yu, MA., Hou, Y., Gong, GH. et al. Effects of industrial storage on the bioreduction capacity of brewer’s yeast. J Ind Microbiol Biotechnol 36, 157–162 (2009). https://doi.org/10.1007/s10295-008-0483-x

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10295-008-0483-x

Keywords

Search

Navigation