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Modulation of tolerance to Cr(VI) and Cr(VI) reduction by sulfate ion in a Candida yeast strain isolated from tannery wastewater

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Journal of Industrial Microbiology & Biotechnology

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Abstract

The main aim of this study was to investigate the influence of the sulfate ion on the tolerance to Cr(VI) and the Cr(VI) reduction in a yeast strain isolated from tannery wastewater and identified as Candida sp. FGSFEP by the D1/D2 domain sequence of the 26S rRNA gene. The Candida sp. FGSFEP strain was grown in culture media with sulfate concentrations ranging from 0 to 23.92 mM, in absence and presence of Cr(VI) [1.7 and 3.3 mM]. In absence of Cr(VI), the yeast specific growth rate was practically the same in every sulfate concentration tested, which suggests that sulfate had no stimulating or inhibiting effect on the yeast cell growth. In contrast, at the two initial Cr(VI) concentrations assayed, the specific growth rate of Candida sp. FGSFEP rose when sulfate concentration increased. Likewise, the greater efficiencies and volumetric rates of Cr(VI) reduction exhibited by Candida sp. FGSFEP were obtained at high sulfate concentrations. Yeast was capable of reducing 100% of 1.7 mM Cr(VI) and 84% of 3.3 mM Cr(VI), with rates of 0.98 and 0.44 mg Cr(VI)/L h, with 10 and 23.92 mM sulfate concentrations, respectively. These results indicate that sulfate plays an important role in the tolerance to Cr(VI) and Cr(VI) reduction in Candida sp. FGSFEP. These findings may have significant implications in the biological treatment of Cr(VI)-laden wastewaters.

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Acknowledgments

C. H. H.-R. and E. C.-U. are fellow holders of a grant from the Comisión de Operación y Fomento de Actividades Académicas, Instituto Politécnico Nacional, Mexico City, Mexico. The authors gratefully acknowledge the financial support provided by the Secretaría de Investigación y Posgrado, IPN. The CONACyT awarded a graduate scholarship to one of the co-authors (F. M. G.-J.).

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  1. Departamento de Ingeniería Bioquímica, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Prolongación de Carpio y Plan de Ayala s/n, Colonia Santo Tomás, CP 11340, Mexico DF, Mexico

    Flor de María Guillén-Jiménez, Liliana Morales-Barrera & Eliseo Cristiani-Urbina

  2. Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Prolongación de Carpio y Plan de Ayala s/n, Colonia Santo Tomás, CP 11340, Mexico DF, Mexico

    Jesús Morales-Jiménez & César Hugo Hernández-Rodríguez

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  1. Flor de María Guillén-Jiménez
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  2. Liliana Morales-Barrera
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  3. Jesús Morales-Jiménez
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Correspondence to Eliseo Cristiani-Urbina.

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Guillén-Jiménez, F.d.M., Morales-Barrera, L., Morales-Jiménez, J. et al. Modulation of tolerance to Cr(VI) and Cr(VI) reduction by sulfate ion in a Candida yeast strain isolated from tannery wastewater. J Ind Microbiol Biotechnol 35, 1277–1287 (2008). https://doi.org/10.1007/s10295-008-0425-7

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