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Phenol and cresol mixture degradation by the yeast Trichosporon cutaneum

  • Original Paper
  • Published:
Journal of Industrial Microbiology & Biotechnology

Abstract

Most industrial wastes contain different organic mixtures, making important the investigation on the microbial destruction of composite substrates. The capability of microbes to remove harmful chemicals from polluted environments strongly depends on the presence of other carbon and energy substrates. The effect of mixtures of phenol- and methyl-substituted phenols (o-, m-, p-cresol) on the growth behaviour and degradation capacity of Trichosporon cutaneum strain was investigated. The cell-free supernatants were analysed by HPLC. It was established that the presence of o-, m- and p- cresol has not prevented complete phenol assimilation but had significant delaying effect on the phenol degradation dynamics. The mutual influence of phenol and p-cresol was investigated. We developed the kinetic model on the basis of Haldane kinetics, which used model parameters from single-substrate experiments to predict the outcome of the two-substrate mixture experiment. The interaction coefficients indicating the degree to which phenol affects the biodegradation of p-cresol and vice versa were estimated. Quantitative estimation of interaction parameters is essential to facilitate the application of single or mixed cultures to the bio-treatment of hazardous compounds.

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Acknowledgments

The support by National Counsel of Science Research of the Bulgarian Ministry of Education and Science under project K 1205/02 is gratefully acknowledged.

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Authors and Affiliations

  1. Institute of Microbiology, Bulgarian Academy of Sciences, Acad.G.Bontchev str., bl.26, 1113, Sofia, Bulgaria

    Z. Alexieva, M. Gerginova, J. Manasiev & N. Shivarova

  2. Institute of Control and System Research, Bulgarian Academy of Sciences, Acad.G.Bontchev str., bl.2, 1113, Sofia, Bulgaria

    P. Zlateva

  3. Department of Biotechnology, University of Food Technologies, Maritza blvd. 26, 4002, Plovdiv, Bulgaria

    A. Krastanov

Authors
  1. Z. Alexieva
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  2. M. Gerginova
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  3. J. Manasiev
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  4. P. Zlateva
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  5. N. Shivarova
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  6. A. Krastanov
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Correspondence to Z. Alexieva.

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Alexieva, Z., Gerginova, M., Manasiev, J. et al. Phenol and cresol mixture degradation by the yeast Trichosporon cutaneum . J Ind Microbiol Biotechnol 35, 1297–1301 (2008). https://doi.org/10.1007/s10295-008-0410-1

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  • DOI: https://doi.org/10.1007/s10295-008-0410-1

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