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Production of xylanase by immobilized Trichoderma reesei SAF3 in Ca-alginate beads

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Journal of Industrial Microbiology & Biotechnology

Abstract

In the present study, the optimum conditions for the production of xylanase by immobilized spores of Trichoderma reesei SAF3 in calcium alginate beads were determined. The operational stability of the beads during xylanase production under semi-continuous fermentation was also studied. The influence of alginate concentration (1, 2, 3, and 4%) and initial cell loading (100, 200, 300, 400, and 500 beads per flask) on xylanase production was considered. The production of xylanase was found to increase significantly with increasing concentration of alginate and reached a maximum yield of 3.12 ± 0.18 U ml−1 at 2% (w/v). The immobilized cells produced xylanase consistently up to 10 cycles and reached a maximum level at the forth cycle (3.36 ± 0.2 U ml−1).

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Authors and Affiliations

  1. Department of Microbiology, Vidyasagar University, Midnapore, 721102, West Bengal, India

    Sanjay Kar, Asish Mandal, Pradeep K. Das Mohapatra, Saptadip Samanta, Bikash R. Pati & Keshab C. Mondal

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  1. Sanjay Kar
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  2. Asish Mandal
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  3. Pradeep K. Das Mohapatra
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  4. Saptadip Samanta
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  5. Bikash R. Pati
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  6. Keshab C. Mondal
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Correspondence to Keshab C. Mondal.

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Kar, S., Mandal, A., Mohapatra, P.K.D. et al. Production of xylanase by immobilized Trichoderma reesei SAF3 in Ca-alginate beads. J Ind Microbiol Biotechnol 35, 245–249 (2008). https://doi.org/10.1007/s10295-007-0292-7

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  • DOI: https://doi.org/10.1007/s10295-007-0292-7

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