Abstract
A potential industrial substrate (liquefied corn starch; LCS) has been employed for successful acetone butanol ethanol (ABE) production. Fermentation of LCS (60 g l−1) in a batch process resulted in the production of 18.4 g l−1 ABE, comparable to glucose: yeast extract based medium (control experiment, 18.6 g l−1 ABE). A batch fermentation of LCS integrated with product recovery resulted in 92% utilization of sugars present in the feed. When ABE was recovered by gas stripping (to relieve inhibition) from the fed-batch reactor fed with saccharified liquefied cornstarch (SLCS), 81.3 g l−1 ABE was produced compared to 18.6 g l−1 (control). In this integrated system, 225.8 g l−1 SLCS sugar (487 % of control) was consumed. In the absence of product removal, it is not possible for C. beijerinckii BA101 to utilize more than 46 g l−1 glucose. A combination of fermentation of this novel substrate (LCS) to butanol together with product recovery by gas stripping may economically benefit this fermentation.
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Acknowledgments
This work was supported in part by Illinois Council on Food and Agricultural Research (CFAR) grant number IDA CF 03E116-1 FSHN and Illinois Corn Marketing Board (ICMB). Authors would like to thank Archer Daniels Midland Company (Decatur, Illinois) for supplying LCS and CSL.
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Ezeji, T.C., Qureshi, N. & Blaschek, H.P. Production of acetone butanol (AB) from liquefied corn starch, a commercial substrate, using Clostridium beijerinckii coupled with product recovery by gas stripping. J Ind Microbiol Biotechnol 34, 771–777 (2007). https://doi.org/10.1007/s10295-007-0253-1
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DOI: https://doi.org/10.1007/s10295-007-0253-1