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Production of laccase by a newly isolated deuteromycete fungus Pestalotiopsis sp. and its decolorization of azo dye

  • Original Paper
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Journal of Industrial Microbiology & Biotechnology

Abstract

The effect of various carbon and nitrogen sources on the production of laccase by newly isolated deuteromycete Pestalotiopsis sp. was tested under liquid-state fermentation. Twenty grams per liter of glucose and 10 g l−1 ammonium tartrate were found to be the optimized concentrations of carbon and nitrogen sources, respectively. The influence of different inducers and inhibitors on the laccase production was also examined. Adding the Cu up to optimum concentration of 2.0 mM in medium (include 20 g l−1 glucose and 10 g l−1 ammonium tartrate), the highest laccase activity of 32.7 ± 1.7 U ml−l was achieved. Cu had to be supplemented after 2 days of growth for its maximal effect, an addition after 6 days of growth, during which laccase activity was dominantly formed, resulted in distinctly reduced laccase activity. In addition, Direct Fast Blue B2RL can be effectively decolorized by crude laccase, the decolorization percentage of which was 88.0 ± 3.2% at pH 4.0 within 12 h. The results suggest that Pestalotiopsis sp. is a high potential producer of the industrially important enzyme laccase.

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Acknowledgments

The experiment was supported by National Natural Science Foundation of China (30470299), the Open Research Foundation of Key Laboratory for Modern Sylvicultural Technology of Zhejiang Province, Zhejiang Forestry College, China, and Key Project of National Science Foundation of China (30430570).

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Authors and Affiliations

  1. School of Life Science, Nanjing University, Nanjing, 210093, China

    Jiejie Hao, Fuqiang Song, Feng Huang, Changlin Yang, Zhijun Zhang, Yi Zheng & Xingjun Tian

  2. Key Laboratory for Modern Sylvicultural Technology of Zhejiang Province, Zhejiang Forestry College, 311300, Zhejiang, China

    Fuqiang Song & Zhijun Zhang

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  1. Jiejie Hao
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  2. Fuqiang Song
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  3. Feng Huang
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  4. Changlin Yang
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  7. Xingjun Tian
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Correspondence to Xingjun Tian.

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Hao, J., Song, F., Huang, F. et al. Production of laccase by a newly isolated deuteromycete fungus Pestalotiopsis sp. and its decolorization of azo dye. J Ind Microbiol Biotechnol 34, 233–240 (2007). https://doi.org/10.1007/s10295-006-0191-3

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  • DOI: https://doi.org/10.1007/s10295-006-0191-3

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