Abstract
Development of novel enzymatic methods for slime deposit control in paper mills requires knowledge of polysaccharide-producing organisms and the polysaccharide structures present in deposits. In this work, 27 polysaccharide-producing bacteria were isolated from slime samples collected from different parts of a paper machine. Most of the isolates produced polysaccharides in liquid culture and nine of them were selected for production of polysaccharides for characterisation. The selected isolates belonged to seven different genera: Bacillus, Brevundimonas, Cytophaga, Enterobacter, Klebsiella, Paenibacillus and Starkeya. Using ribotyping, partial 16S rDNA sequencing, physiological tests and fatty acid analysis, four of the nine isolates: Bacillus cereus, Brevundimonas vesicularis, K. pneumoniae and P. stellifer were identified to the species level. Production of polysaccharides by the selected isolates varied between 0.07 and 1.20 g L−1, the highest amount being produced by B. vesicularis. The polysaccharides were heteropolysaccharides with varying proportions of galactose, glucose mannose, rhamnose fucose and uronic acids.
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Acknowledgements
The work described has been carried out with financial support from the Commission of European Communities, specific RTD programme “Competitive and Sustainable Growth”, G1RD-CT2000-00387, “Eco-efficient novel enzymatic concepts for slime control in pulp and paper processing (Slimezymes)”. It does not necessarily reflect the Commission’s views and in no way anticipates the Commission’s future policy in this area.
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Rättö, M., Suihko, M.L. & Siika-aho, M. Polysaccharide-producing bacteria isolated from paper machine slime deposits. J IND MICROBIOL BIOTECHNOL 32, 109–114 (2005). https://doi.org/10.1007/s10295-005-0210-9
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DOI: https://doi.org/10.1007/s10295-005-0210-9