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Genetic guidelines for the conservation of the endangered polyploid Centaurea borjae (Asteraceae)

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Abstract

Appropriate management of species of conservation concern requires designing strategies that should include genetic information as small population size and restricted geographic range can reduce genetic variation. We used AFLPs to investigate genetic variation within and among populations of the endangered narrow endemic Centaurea borjae, and found no evidence for genetic impoverishment despite its <40 km range and potential for vegetative propagation. Genetic variation was comparable to other plants with similar life history (88 % occurring within populations) and potential clone mates were less frequent than expected. Nonetheless, populations separated by few hundred meters showed signs of significant genetic differentiation suggesting low gene flow between them. Our results suggested that the three geographically closer populations located at the center of the range might be treated as a single management unit, while the remaining ones could be considered independent units. We found evidence of fine-scale spatial genetic structure up to 80 m indicating that the collection of germplasm for ex-situ conservation should focus on individuals separated >80 m to maximize genetic variation.

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References

  • Abbott RJ, Ireland HE, Rogers HJ (2007) Population decline despite high genetic diversity in the new allopolyploid species Senecio cambrensis (Asteraceae). Mol Ecol 16:1023–1033

    Article  PubMed  CAS  Google Scholar 

  • Aguilar R, Quesada M, Ashworth L, Herrerias-Diego Y, Lobo J (2008) Genetic consequences of habitat fragmentation in plant populations: susceptible signals in plant traits and methodological approaches. Mol Ecol 17:5177–5188

    Article  PubMed  Google Scholar 

  • Andreakis N, Kooistra WHCF, Procaccini G (2009) High genetic diversity and connectivity in the polyploid invasive seaweed Asparagopsis taxiformis (Bonnemaisoniales) in the Mediterranean, explored with microsatellite alleles and multilocus genotypes. Mol Ecol 18:212–226

    Article  PubMed  Google Scholar 

  • Arnaud-Haond S, Duarte CM, Alberto F, Serrao EA (2007) Standardizing methods to address clonality in population studies. Mol Ecol 16:5115–5139

    Article  PubMed  CAS  Google Scholar 

  • Bañares A, Blanca G, Güemes J, Moreno JC, Ortiz S (2004) Atlas y Libro Rojo de la Flora Vascular Amenazadas de España. Dirección General de Conservación de la Naturaleza, Madrid

    Google Scholar 

  • Barnaud A, Houliston GJ (2010) Population genetics of the threatened tree daisy Olearia gardneri (Asteraceae), conservation of a critically endangered species. Conserv Genet 11:1515–1522

    Article  Google Scholar 

  • Bonin A, Bellemain E, Bronken Eidesen P (2004) How to track and assess genotyping errors in population genetics studies. Mol Ecol 13:3261–3273

    Article  PubMed  CAS  Google Scholar 

  • Bonin A, Pompanon F, Taberlet P (2005) Use of amplified fragment length polymorphism (AFLP) markers in surveys of vertebrate diversity. Methods Enzymol 395:145–161

    Article  PubMed  CAS  Google Scholar 

  • Bonin A, Ehrich D, Manel S (2007) Statistical analysis of amplified fragment length polymorphism data: a toolbox for molecular ecologists and evolutionists. Mol Ecol 16:3737–3758

    Article  PubMed  CAS  Google Scholar 

  • Bruvo R, Michiels NK, D’Souza TG, Schulenburg H (2004) A simple method for the calculation of microsatellite genotype distances irrespective of ploidy level. Mol Ecol 13:2101–2106

    Article  PubMed  CAS  Google Scholar 

  • Chung MG, Epperson BK (1999) Spatial genetic structure of clonal and sexual reproduction in populations of Adenophora grandiflora (Campanulaceae). Evolution 53:1068–1078

    Article  CAS  Google Scholar 

  • Chung MY, Suh Y, Lopez-Pujol J, Nason JD, Chung MG (2005) Clonal and fine-scale genetic structure in populations of a restricted Korean endemic, Hosta jonesii (Liliaceae) and the implications for conservation. Ann Bot 96:279–288

    Article  PubMed  Google Scholar 

  • Colas B, Olivieri I, Riba M (1997) Centaurea corymbosa, a cliff-dwelling species tottering on the brink of extinction: A demographic and genetic study. Proc Natl Acad Sci USA 94:3471–3476

    Article  PubMed  CAS  Google Scholar 

  • Cole CT (2003) Genetic variation in rare and common plants. Annu Rev Ecol Evol S 34:213–237

    Article  Google Scholar 

  • Corander J, Siren J, Arjas E (2008) Bayesian spatial modeling of genetic population structure. Comput Stat 23:111–129

    Article  Google Scholar 

  • Cousens R, Dytham C, Law R (2008) Dispersal in plants: a population perspective. Oxford University Press, Oxford

    Google Scholar 

  • Crandall KA, Bininda-Emonds ORP, Mace GM, Wayne RK (2000) Considering evolutionary processes in conservation biology. Trends Ecol Evol 15:290–295

    Article  PubMed  Google Scholar 

  • Despres L, Loriot S, Gaudeul M (2002) Geographic pattern of genetic variation in the European globeflower Trollius europaeus L. (Ranunculaceae) inferred from amplified fragment length polymorphism markers. Mol Ecol 11:2337–2347

    Article  PubMed  CAS  Google Scholar 

  • Douhovnikoff V, Dodd RS (2003) Intra-clonal variation and a similarity threshold for identification of clones: application to Salix exigua using AFLP molecular markers. Theor Appl Genet 106:1307–1315

    PubMed  CAS  Google Scholar 

  • Dyer RJ (2009) GeneticStudio: a suite of programs for spatial analysis of genetic-marker data. Mol Ecol Res 9:110–113

    Article  Google Scholar 

  • Dyer RJ, Nason JD (2004) Population Graphs: the graph theoretic shape of genetic structure. Mol Ecol 13:1713–1727

    Article  PubMed  Google Scholar 

  • Dyer RJ, Nason JD, Garrick RC (2010) Landscape modelling of gene flow: improved power using conditional genetic distance derived from the topology of population networks. Mol Ecol 19:3746–3759

    Article  PubMed  Google Scholar 

  • Ellstrand NC, Elam DR (1993) Population genetic consequences of small population size: implications for plant conservation. Annu Rev Ecol Evol Syst 24:217–242

    Article  Google Scholar 

  • Evanno G, Regnaut S, Goudet J (2005) Detecting the number of clusters of individuals using the software structure: a simulation study. Mol Ecol 14:2611–2620

    Article  PubMed  CAS  Google Scholar 

  • Excoffier L, Smouse PE, Quattro JM (1992) Analysis of molecular variance inferred from metric distances among DNA haplotypes: application to human mitochondrial DNA restriction data. Genetics 21:479–491

    Google Scholar 

  • Excoffier L, Hofer T, Foll M (2009) Detecting loci under selection in a hierarchically structured population. Heredity 103:285–298

    Article  PubMed  CAS  Google Scholar 

  • Falush D, Stephens M, Pritchard JK (2003) Inference of population structure using multilocus genotype data: linked loci and correlated allele frequencies. Genetics 164:1567–1587

    PubMed  CAS  Google Scholar 

  • Fernández Casas J, Susanna A (1986) Monografía de la sección Chamaecyanus Willk. del género Centaurea. Treballs de l’Institut Botànic de Barcelona 10:1–174

    Google Scholar 

  • Foll M, Gaggiotti O (2008) A genome-scan method to identify selected loci appropriate for both dominant and codominant markers: a bayesian perspective. Genetics 180:977–993

    Article  PubMed  Google Scholar 

  • Font M (2007) Poliploïdia, filogènia i biogeografia en Centaurea L. secció Acrocentron (Cass.) DC., Universitat de Barcelona, Barcelona

  • Font M, Garcia-Jacas N, Vilatersana R, Roquet C, Susanna A (2009) Evolution and biogeography of Centaurea section Acrocentron inferred from nuclear and plastid DNA sequence analyses. Ann Bot 103:985–997

    Article  PubMed  CAS  Google Scholar 

  • Frankel OH, Brown AHD, Burdon JJ (1995) The conservation of plant biodiversity. Cambridge University Press, Cambridge

    Google Scholar 

  • Frankham R (2005) Genetics and extinction. Biol Conserv 126:131–140

    Article  Google Scholar 

  • Frankham R (2010) Challenges and opportunities of genetic approaches to biological conservation. Biol Conserv 143:1919–1927

    Article  Google Scholar 

  • Frankham R, Briscoe DA, Ballou JD (2002) Introduction to conservation genetics. Cambridge University Press, Cambridge

    Book  Google Scholar 

  • Garcia-Jacas N, Susanna A (1992) Karyological notes on Centaurea sect. Acrocentron (Asteraceae). Plant Syst Evol 179:1–18

    Article  Google Scholar 

  • Garcia-Verdugo C, Fay MF, Granado-Yela C, Rubio de Casas R, Balaguer L, Besnard G, Vargas P (2009) Genetic diversity and differentiation processes in the ploidy series of Olea europaea L.: a multiscale approach from subspecies to insular populations. Mol Ecol 18:454–467

    Article  PubMed  CAS  Google Scholar 

  • Garroway CJ, Bowman J, Carr D, Wilson PJ (2008) Applications of graph theory to landscape genetics. Evol Appl 1:620–630

    Google Scholar 

  • Ghazoul J (2005) Pollen and seed dispersal among dispersed plants. Biol Rev 80:413–443

    Article  PubMed  Google Scholar 

  • Gitzendanner MA, Soltis PS (2000) Patterns of genetic variation in rare and widespread plant congeners. Am J Bot 87:783–792

    Article  PubMed  CAS  Google Scholar 

  • Gomez C, Espadaler X (1998) Myrmecochorous dispersal distances: a world survey. J Biogeogr 25:573–580

    Article  Google Scholar 

  • Gómez-Orellana Rodríguez L (2004) Cenaturea borjae Valdés Verm. & Rivas Goday. In: Bañares A, Blanca G, Güemes J, Moreno JC, Ortiz S (eds) Atlas y Libro Rojo de la Flora Vascular Amenazada de España. Dirección General de Conservación de la Naturaleza, Madrid, pp 632–633

    Google Scholar 

  • Gómez-Orellana Rodríguez L (2011) Centaurea borjae (ed. 2011 I). IUCN red list of threatened species. Version 2011.1

  • Hamrick JL, Godt MJW (1996) Conservation genetics of endemic plant species. In: Avise JC, Hamrick JL (eds) Conservation genetics: case histories from nature. Chapman & Hall, New York, pp 281–304

    Google Scholar 

  • Hardy OJ, Vekemans X (2002) SPAGEDi: a versatile computer program to analyse spatial genetic structure at the individual or population levels. Mol Ecol Notes 2:618–620

    Article  Google Scholar 

  • Hardy OJ, González-Martinez SC, Fréville H, Boquien G, Mignot A, Colas B, Olivieri I (2004) Fine-scale genetic structure and gene dispersal in Centaurea corymbosa (Asteraceae) I. Pattern of pollen dispersal. J Evol Biol 17:795–806

    Article  PubMed  CAS  Google Scholar 

  • Honnay O, Jacquemyn H (2008) A meta-analysis of the relation between mating system, growth form and genotypic diversity in clonal plant species. Evol Ecol 22:299–312

    Article  Google Scholar 

  • Hubisz MJ, Falush D, Stephens M, Pritchard JK (2009) Inferring weak population structure with the assistance of sample group information. Mol Ecol Res 9:1322–1332

    Article  Google Scholar 

  • Imbert E (2006) Dispersal by ants in Centaurea corymbosa (Asteraceae): what is the elaiosome for? Plant Species Biol 21:109–117

    Article  Google Scholar 

  • Izco J, Rodríguez Oubiña J, Romero MI, Amigo J, Pulgar I, Gomez M (2003) Flora endémica de A Coruña (España): Centaurea borjae y Centaurea ultreiae. Diputación Provincial A Coruña, A Coruña

  • Jakobsson M, Rosenberg NA (2007) CLUMPP: a cluster matching and permutation program for dealing with label switching and multimodality in analysis of population structure. Bioinformatics 23:1801–1806

    Article  PubMed  CAS  Google Scholar 

  • Jensen JL, Bohonak AJ, Kelley ST (2005) Isolation by distance, web service. BMC Genet 6:13

    Article  PubMed  Google Scholar 

  • Jump AS, Peñuelas J (2007) Extensive spatial genetic structure revealed by AFLP but not SSR molecular markers in the wind-pollinated tree, Fagus sylvatica. Mol Ecol 16:925–936

    Article  PubMed  CAS  Google Scholar 

  • Kato S, Iwata H, Tsumura Y, Mukai Y (2011) Genetic structure of island populations of Prunus lannesiana var. speciosa revealed by chloroplast DNA, AFLP and nuclear SSR loci analyses. J Plant Res 124:11–23

    Article  PubMed  Google Scholar 

  • Kim SC, Lee C, Santos-Guerra A (2005) Genetic analysis and conservation of the endangered Canary Island woody sow-thistle, Sonchus gandogeri (Asteraceae). J Plant Res 118:147–153

    Article  PubMed  CAS  Google Scholar 

  • Kloda JM, Dean PDG, Maddren C, MacDonald DW, Mayes S (2008) Using principle component analysis to compare genetic diversity across polyploidy levels within plant complexes: an example from British Restharrows (Ononis spinosa and Ononis repens). Heredity 100:253–260

    Article  PubMed  CAS  Google Scholar 

  • Kosman E (2003) Nei’s gene diversity and the index of average differences are identical measures of diversity within populations. Plant Pathol 52:533–535

    Article  Google Scholar 

  • Kosman E, Leonard KJ (2005) Similarity coefficients for molecular markers in studies of genetic relationships between individuals for haploid, diploid, and polyploid species. Mol Ecol 14:415–424

    Article  PubMed  CAS  Google Scholar 

  • Kruckeberg AR, Rabinowitz D (1985) Biological aspects of endemism in higher plants. Annu Rev Ecol Evol Syst 16:447–479

    Article  Google Scholar 

  • Lavergne S, Thompson JD, Garnier E, Debussche M (2004) The biology and ecology of narrow endemic and widespread plants: a comparative study of trait variation in 20 congeneric pairs. Oikos 107:505–518

    Article  Google Scholar 

  • Li XX, Ding XY, Chu BH, Zhou Q, Ding G, Gu S (2008) Genetic diversity analysis and conservation of the endangered Chinese endemic herb Dendrobium officinale Kimura et Migo (Orchidaceae) based on AFLP. Genetica 133:159–166

    Article  PubMed  CAS  Google Scholar 

  • Loiselle BA, Sork VL, Nason J, Graham C (1995) Spatial genetic-structure of a tropical understory shrub, Psychotria officinalis (Rubiaceae). Am J Bot 82:1420–1425

    Article  Google Scholar 

  • Marko PB, Hart MW (2011) The complex analytical landscape of gene flow inference. Trends Ecol Evol 26:448–456

    Article  PubMed  Google Scholar 

  • Mayrose I, Zhan SH, Rothfels CJ, Magnuson-Ford K, Bajer MS, Rieseberg LH, Otto SP (2011) Recently formed polyploid plants diversify at lower rates. Science 333:1257

    Article  PubMed  CAS  Google Scholar 

  • Mba C, Tohme J (2005) Use of AFLP markers in surveys of plant diversity. Methods Enzymol 395:177–201

    Article  PubMed  CAS  Google Scholar 

  • Meirmans PG, Van Tienderen PH (2004) Genotype and Genodive: two programs for the analysis of genetic diversity of asexual organisms. Mol Ecol Notes 4:792–794

    Article  Google Scholar 

  • Ministerio de Medio Ambiente y Medio Rural y Marino (2011) Real Decreto 139/2011, de 4 de febrero, para el desarrollo del Listado de Especies Silvestres en Régimen de Protección Especial y del Catálogo Español de Especies Amenazadas. Boletin Oficial del Estado 46:20912–20951

    Google Scholar 

  • Minor ES, Urban DL (2007) Graph theory as a proxy for spatially explicit population models in conservation planning. Ecol Appl 17:1771–1782

    Article  PubMed  Google Scholar 

  • Morden CW, Loeffler WF (1999) Fragmentation and genetic differentiation among subpopulations of the endangered Hawaiian mint Haplostachys haplostachya (Lamiaceae). Mol Ecol 8:617–625

    Article  Google Scholar 

  • Moritz C (1994) Defining “evolutionarily-significant-units” for conservation. Trends Ecol Evol 9:373–375

    Article  PubMed  CAS  Google Scholar 

  • Nybom H (2004) Comparison of different nuclear DNA markers for estimating intraspecific genetic diversity in plants. Mol Ecol 13:1143–1155

    Article  PubMed  CAS  Google Scholar 

  • Obbard DJ, Harris SA, Pannell JR (2006) Simple allelic-phenotype diversity and differentiation statistics for allopolyploids. Heredity 97:296–303

    Article  PubMed  CAS  Google Scholar 

  • Palacios C, Kresovich S, Gonzalez-Candelas F (1999) A population genetic study of the endangered plant species Limonium dufourii (Plumbaginaceae) based on amplified fragment length polymorphism (AFLP). Mol Ecol 8:645–657

    Article  CAS  Google Scholar 

  • Peakall R, Smouse PE (2006) GENALEX 6: genetic analysis in Excel. Population genetic software for teaching and research. Mol Ecol Notes 6:288–295

    Article  Google Scholar 

  • Peters MD, Xiang QY, Thomas DT, Stucky J, Whiteman NK (2009) Genetic analyses of the federally endangered Echinacea laevigata using amplified fragment length polymorphisms (AFLP)—inferences in population genetic structure and mating system. Conserv Genet 10:1–4

    Article  CAS  Google Scholar 

  • Pisanu S, Filigheddu R, Farris E (2009) The conservation status of an endemic species of northern Sardinia: Centaurea horrida Badaro (Asteraceae). Plant Biosyst 143:275–282

    Article  Google Scholar 

  • Pritchard JK, Stephens M, Donnelly P (2000) Inference of population structure using multilocus genotype data. Genetics 155:945–959

    PubMed  CAS  Google Scholar 

  • Reed DH, Frankham R (2003) Correlation between fitness and genetic diversity. Conserv Biol 17:230–237

    Article  Google Scholar 

  • Roiloa SR, Retuerto R (2006) Physiological integration ameliorates effects of serpentine soils in the clonal herb Fragaria vesca. Physiol Plantarum 128:662–676

    Article  CAS  Google Scholar 

  • Sebbenn AM, Carvalho ACM, Freitas MLM, Moraes SMB, Gaino APSC, da Silva JM, Jolivet C, Moraes MLT (2011) Low levels of realized seed and pollen gene flow and strong spatial genetic structure in a small, isolated and fragmented population of the tropical tree Copaifera langsdorffii Desf. Heredity 106:134–145

    Article  PubMed  CAS  Google Scholar 

  • Silvertown J (2008) The evolutionary maintenance of sexual reproduction: evidence from the ecological distribution of asexual reproduction in clonal plants. Int J Plant Sci 169:157–168

    Article  Google Scholar 

  • Soltis PS, Soltis DE (2000) The role of genetic and genomic attributes in the success of polyploids. Proc Natl Acad Sci USA 97:7051–7057

    Article  PubMed  CAS  Google Scholar 

  • Soñora X (1994) Nueva localidad de Centaurea borjae Valdés-Bermejo & Rivas Goday. Lazaroa 14:183–184

    Google Scholar 

  • Sözen E, Özaydin B (2009) A preliminary study of the genetic diversity of the critically endangered Centaurea nivea (Asteraceae). Ann Bot Fenn 46:541–548

    Article  Google Scholar 

  • Sözen E, Özaydin B (2010) A study of genetic variation in endemic plant Centaurea wiedemanniana by using RAPD markers. Ekoloji 19:1–8

    Article  Google Scholar 

  • Stefenon VM, Gailing O, Finkeldey R (2008) Genetic structure of plantations and the conservation of genetic resources of Brazilian pine (Araucaria angustifolia). For Ecol Manag 255:2718–2725

    Article  Google Scholar 

  • Stehlik I, Holderegger R (2000) Spatial genetic structure and clonal diversity of Anemone nemorosa in late successional deciduous woodlands of Central Europe. J Ecol 88:424–435

    Article  Google Scholar 

  • Takahashi Y, Takahashi H, Maki M (2011) Comparison of genetic variation and differentiation using microsatellite markers among three rare threatened and one widespread toad lily species of Tricyrtis section Flavae (Convallariaceae) in Japan. Plant Species Biol 26:13–23

    Article  Google Scholar 

  • Tallmon DA, Luikart G, Waples RS (2004) The alluring simplicity and complex reality of genetic rescue. Trends Ecol Evol 19:489–496

    Article  PubMed  Google Scholar 

  • Tero N, Aspi J, Siikamäki P, Jäkäläniemi A, Tuomi J (2003) Genetic structure and gene flow in a metapopulation of and endangered plant species, Silene tatarica. Mol Ecol 12:2073–2085

    Article  PubMed  CAS  Google Scholar 

  • Valdés-Bermejo E, Agudo Mata MP (1983) Estudios cariológicos de especies ibéricas del género Centaurea L. (Compositae). I. Anales del Jardín Botánico de Madrid 40:119–142

    Google Scholar 

  • Valdes-Bermejo E, Rivas Goday S (1978) Estudios en el género Centaurea L. (Compositae): C. borjae sp. nov. (Sect. Borjae Sect. Nov). Anales del Instituto Botánico AJ. Cavanilles 85:159–164

    Google Scholar 

  • Vallejo-Marin M, Dorken ME, Barrett SCH (2010) The ecological and evolutionary consequences of clonality for plant mating. Annu Rev Ecol Evol Syst 41:193–213

    Article  Google Scholar 

  • Vekemans X, Hardy OJ (2004) New insights from fine-scale spatial genetic structure analyses in plant populations. Mol Ecol 13:921–935

    Article  PubMed  CAS  Google Scholar 

  • Vilatersana R, Susanna A, Brochmann C (2007) Genetic variation in Femeniasia (Compositae, Cardueae), an endemic and endangered monotypic genus from the Balearic Islands (Spain). Bot J Linn Soc 153:97–107

    Article  Google Scholar 

  • Vos P, Hogers R, Bleeker M, Reijans M, Van de Lee T, Hornes M, Frijters A, Pot J, Peleman J, Kuiper M, Zabeau M (1995) AFLP: a new technique for DNA fingerprinting. Nucl Acids Res 23:4407–4414

    Article  PubMed  CAS  Google Scholar 

  • Whitlock MC, McCauley DE (1999) Indirect measures of gene flow and migration: FST ≠ 1/(4 Nm + 1). Heredity 82:117–125

    Article  PubMed  Google Scholar 

  • Willi Y, Van Buskirk J, Hoffmann AA (2006) Limits to the adaptive potential of small populations. Annu Rev Ecol Evol Syst 37:433–458

    Article  Google Scholar 

  • Winfield MO, Arnold GM, Cooper F, Le Ray M, White J, Karp A, Edwards KJ (1998) A study of genetic diversity in Populus nigra subsp. betulifolia in the upper seven area of the UK using AFLP markers. Mol Ecol 7:3–10

    Article  CAS  Google Scholar 

  • Yan XB, Guo YX, Zhao C, Liu FY, Lu BR (2009) Intra-population genetic diversity of two wheatgrass species along altitude gradients on the Qinghai-Tibetan Plateau: its implication for conservation and utilization. Conserv Genet 10:359–367

    Article  Google Scholar 

  • Zeng LY, Xu LL, Tang SQ, Tersing T, Geng YP, Zhong Y (2010) Effect of sampling strategy on stimulation of fine-scale spatial genetics structure in Androsace tapete (Primulaceae), an alpine plant endemic to Qinghai-Tibetan Plateau. J Syst Evol 48:257–264

    Article  Google Scholar 

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Acknowledgments

This research was supported by the project 07MDS031103PR (Xunta de Galicia). We deeply appreciate the help of Maria Quintela with network, the outlier loci detection and the STRUCTURE analysis. We also thank three anonymous reviewers for insightful comments on an earlier version of the manuscript.

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  1. Área de Ecología, Facultad de Ciencias, Universidad da Coruña, Campus A Zapateira s/n, 15071, A Coruña, Spain

    Lua Lopez & Rodolfo Barreiro

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Correspondence to Lua Lopez.

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Lopez, L., Barreiro, R. Genetic guidelines for the conservation of the endangered polyploid Centaurea borjae (Asteraceae). J Plant Res 126, 81–93 (2013). https://doi.org/10.1007/s10265-012-0497-3

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