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Morphological and molecular evidence for natural hybridization in sympatric population of Roscoea humeana and R. cautleoides (Zingiberaceae)

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Abstract

The sympatric occurrence of some species in Roscoea is very common, but little information is available on natural hybridization. However, some intermediate individuals were found on the sympatric population of Roscoea humeana and R. cautleoides at Ganhaizi population in northwestern Yunnan Province, China. We suspected that these intermediate individuals were the hybrids of R. humeana and R. cautleoides from the previous evidence, but could not confirm them. In this study, morphometric analysis was followed by examination of HAT-RAPD polymorphisms to determine the occurrence of natural hybridization between sympatric R. humeana and R. cautleoides. The results showed that most morphological characters of the putative hybrids were found to be intermediate between those of R. humeana and R. cautleoides. Meanwhile, molecular analysis confirmed that the morphological intermediates were derived from hybridization between the two species. From the analysis of the NewHybrids, the hybridization individuals were mainly F1s. These results indicated that interspecific hybridization between R. humeana and R. cautleoides indeed occurred in sympatric population.

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Acknowledgments

We are grateful to the two reviewers for their valuable comments. This work was supported by the National Basic Research Program of China (973 Program) (2007CB411603), the CAS/SEFEA International Partnership Program for Creative Research Teams, the Fund for Top One Hundred Young Scientists of the Chinese Academy of Sciences, the Knowledge Innovation Program of the Chinese Academy of Sciences (KSCX2-YW-Z-0903), and National Natural Science Foundation of China (31100179).

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Authors and Affiliations

  1. Key Laboratory of Tropical Forest Ecology, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Menglun Town, Mengla County, Yunnan, 666303, China

    Guang-Hui Du, Zhi-Qiang Zhang & Qing-Jun Li

  2. Graduate University of Chinese Academy of Sciences, Beijing, 100039, China

    Guang-Hui Du

  3. Key Laboratory of Biodiversity and Biogeography, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, 650204, China

    Zhi-Qiang Zhang

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  1. Guang-Hui Du
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  2. Zhi-Qiang Zhang
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Correspondence to Qing-Jun Li.

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G.-H. Du and Z.-Q. Zhang are the first two authors contributed equally to this paper.

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Du, GH., Zhang, ZQ. & Li, QJ. Morphological and molecular evidence for natural hybridization in sympatric population of Roscoea humeana and R. cautleoides (Zingiberaceae). J Plant Res 125, 595–603 (2012). https://doi.org/10.1007/s10265-012-0478-6

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  • DOI: https://doi.org/10.1007/s10265-012-0478-6

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