Abstract
In three temperature-sensitive mutants of Arabidopsis, root redifferentiation 1 (rrd1), rrd2, and root initiation defective 4 (rid4), formation of fasciated lateral roots was previously observed under high temperature conditions of 28°C. When lateral roots were induced from explants of very young seedlings of these mutants by culture with exogenously supplied auxin at 28°C, expansion of lateral root primordia leading to lateral root fasciation occurred reproducibly and semi-synchronously with a high frequency. This experimental system allowed us to examine how radial organization of root tissues is altered in association with expansion of primordia. Analysis with various tissue-specific reporter genes indicated that in the fasciated lateral roots, cell files of the stele are increased markedly while the numbers of cortical and epidermal cell layers are not changed. This suggests that radial organization during root primordium development involves a mechanism that makes outer layer patterning more robust than inner layer patterning against unusual enlargement of the morphogenetic field.
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Acknowledgments
We thank Dr. Tom J. Guilfoyle (University of Missouri) for providing the DR5::GUS line, Drs. Philip N. Benfey (Duke University), Hidehiro Fukaki (Kobe University), and Minako Ueda (Nara Institute of Science and Technology) for the SCR::GFP and SHR::GFP lines, and Drs. Ben Scheres (Utrecht University), Jirí Friml (Ghent University), Masahiko Furutani (Nara Institute of Science and Technology) for the PIN reporter lines. This work was supported by a Grant-in-Aid for Scientific Research on Priority Areas (No. 19060001 to M.S.) of the Ministry of Education, Culture, Sports, Science and Technology, Japan and also by a Grant-in-Aid for JSPS Fellows (No. 218676 to K.O.) from the Japan Society for the Promotion of Science.
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Otsuka, K., Sugiyama, M. Tissue organization of fasciated lateral roots of Arabidopsis mutants suggestive of the robust nature of outer layer patterning. J Plant Res 125, 547–554 (2012). https://doi.org/10.1007/s10265-011-0471-5
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DOI: https://doi.org/10.1007/s10265-011-0471-5