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Hydrogen peroxide permeability of plasma membrane aquaporins of Arabidopsis thaliana

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Abstract

Although aquaporins have been known to transport hydrogen peroxide (H2O2) across cell membranes, the H2O2-regulated expression patterns and the permeability of every family member of the plasma membrane intrinsic protein (PIP) toward H2O2 have not been determined. This study investigates the H2O2-regulated expression levels of all plasma membrane aquaporins of Arabidopsis thaliana (AtPIPs), and determines the permeability of every AtPIP for H2O2 in yeast. Hydrogen peroxide treatment of Arabidopsis down-regulated the expression of AtPIP2 subfamily in roots but not in leaves, whereas the expression of AtPIP1 subfamily was not affected by H2O2 treatment. The growth and survival of yeast cells that expressed AtPIP2;2, AtPIP2;4, AtPIP2;5, or AtPIP2;7 was reduced in the presence of H2O2, while the growth of yeast cells expressing any other AtPIP family member was not affected by H2O2. These results show that only certain isoforms of AtPIPs whose expression is regulated by H2O2 treatment are permeable for H2O2 in yeast cells, and suggest that the integrated regulation of aquaporin expression by H2O2 and the capacity of individual aquaporin to transport H2O2 are important for plant response to H2O2.

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Acknowledgments

The mutant yeast strain was a kind gift from Dr. S. Hohmann (Göteborg University, Sweden). This work was supported in part by a grant from the National Research Foundation (NRF) of Korea grant funded by the Ministry of Education, Science and Technology (MEST) to the Agricultural Plant Stress Research Center (APSRC, R11-2001-092-05002-0) of Chonnam National University and by World Class University program (R32-20047-0) through the NRF of Korea funded by the MEST.

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Authors and Affiliations

  1. Department of Plant Biotechnology and Agricultural Plant Stress Research Center, College of Agriculture and Life Sciences, Chonnam National University, 300 Yongbong-dong, Buk-gu, Gwangju, 500-757, South Korea

    Cortwa Hooijmaijers, Ji Ye Rhee, Kyung Jin Kwak, Gap Chae Chung & Hunseung Kang

  2. Institute of Plant Science and Resources, Okayama University, Chuo 2-20-1, Kurashiki, 710-0046, Japan

    Ji Ye Rhee & Maki Katsuhara

  3. Division of Applied Biology, Faculty of Textile Science and Technology, Shinsu University, Ueda, Nagano, 386-8567, Japan

    Tomoaki Horie

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  1. Cortwa Hooijmaijers
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  2. Ji Ye Rhee
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  3. Kyung Jin Kwak
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  4. Gap Chae Chung
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  5. Tomoaki Horie
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  6. Maki Katsuhara
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  7. Hunseung Kang
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Correspondence to Hunseung Kang.

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Hooijmaijers, C., Rhee, J.Y., Kwak, K.J. et al. Hydrogen peroxide permeability of plasma membrane aquaporins of Arabidopsis thaliana . J Plant Res 125, 147–153 (2012). https://doi.org/10.1007/s10265-011-0413-2

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