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Damage of PS II during senescence of Spirodela polyrrhiza explants under long-day conditions and its prevention by 6-benzyladenine

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Abstract

The chlorophyll a (Chl a) fluorescence technique was applied to investigate damage of PS II during senescence of excised half-fronds in Spirodela polyrrhiza P143. The green explants showed a typical Chl a fluorescence transient, OJIP. After cultivation of explants under long-day conditions for 8 days, all the J, I, and P steps disappeared, but a clear K band, an indication of senescence, was observed. JIP-test showed that at this time point, the photosynthetic performance index (PI) dropped to zero and the active reaction center (RC) per leaf cross-section (RC/CS) declined to 18%. As the oxygen-evolving complex (OEC) and the chlorophyll content all remained above 42%, it is proposed that the decline in RC contributes more to the appearance of the K band. Supplementation of 6-benzyladenine (6-BA) into the medium at the beginning of cultivation caused dramatic increase in PI, OEC, RC/CS, and chlorophyll content, and at any time before the 8th day reversed the senescence process of the explants. When 6-BA was added after 8 days of cultivation, the PI did not increase anymore, RC/CS and OEC were maintained at 22% and above 40%, respectively, and chlorophyll content decreased continuously further. These data support a view that the decline in RC is crucial for initiation of the irreversible senescence phase of explants cultivated under long-day conditions.

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Abbreviations

6-BA:

6-Benzyladenine

ABS:

Absorption

Chl a:

Chlorophyll a

CS:

Cross section of the sample

FO and FM:

Initial and maximum Chl a fluorescence

F J :

The fluorescence intensity at 2 ms (J-step)

K, J, I:

Intermediate steps of Chl a fluorescence rise between FO and maximal fluorescence (P or FM)

OEC:

Oxygen evolving complex

PI:

Performance index

PS II:

Photosystem II

RC:

Reaction center

V :

The relative variable fluorescence for the normalization between FO and FM

W :

The relative variable fluorescence for the normalization between FO and FJ

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Acknowledgements

This research was supported by the National Natural Science Foundation of China (No. 39970077).

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Authors and Affiliations

  1. Department of Plant Biology and Ecology, College of Life Sciences, Nankai University, 300071, Tianjin, China

    Qingdai Liu, Yerong Zhu, Hanlin Tao, Ningning Wang & Yong Wang

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  1. Qingdai Liu
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  3. Hanlin Tao
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  4. Ningning Wang
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Correspondence to Yong Wang.

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Liu, Q., Zhu, Y., Tao, H. et al. Damage of PS II during senescence of Spirodela polyrrhiza explants under long-day conditions and its prevention by 6-benzyladenine. J Plant Res 119, 145–152 (2006). https://doi.org/10.1007/s10265-006-0259-1

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