Abstract
Various alcohol dehydrogenase (ADH) isoenzymes and aldehyde dehydrogenase (ALDH) exist in the human esophageal mucosa. In our last experiments we have shown that ADH and ALDH are present also in the esophageal cancer cells. Moreover, the activities of total ADH and class IV isoenzymes were significantly higher in cancer tissue than in healthy mucosa, which suggests that these changes may be reflected by enzyme activity in the serum. Therefore, we measured the activity of total alcohol dehydrogenase, and classes I–IV of this enzyme and aldehyde dehydrogenase in the sera of patients with this cancer. Serum samples were taken for routine biochemical investigation from 67 patients with esophageal cancer before treatment. Total ADH activity was measured by photometric method with p-nitrosodimethylaniline (NDMA) as a substrate and ALDH activity by the fluorometric method with 6-methoxy-2-naphtaldehyde as a substrate. For the measurement of the activity of class I and II isoenzymes, we employed the fluorometric methods, with class-specific fluorogenic substrates. The activity of class III alcohol dehydrogenase was measured by the photometric method with formaldehyde and class IV with m-nitrobenzaldehyde as a substrate. A statistically significant increase of class IV alcohol dehydrogenase isoenzymes was found in the sera of cancer patients. The median activity of this class isoenzyme in the total cancer group increased by about 26.5% (7.42 mU/l) in comparison to the control level (5.46 mU/l). The total alcohol dehydrogenase activity was significantly higher (30%) among patients with cancer. The activities of other tested ADH isoenzymes and total ALDH were unchanged. The activity of the class I ADH isoenzyme was significantly higher in the sera of drinkers with esophageal cancer than non-drinking patients. The increased total activity of alcohol dehydrogenase and class IV isoenzyme in the sera of patients with esophageal cancer probably can be caused by release of this isoenzyme from cancer cells or might be stimulated by alcohol drinking.
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References
Pares X, Martinez SE, Allali-Hassani A, Borras E, Farres J, Martras S, Rosell A, Vaglenova J (2001) Distribution of alcohol dehydrogenase in human organs: relevance for alcohol metabolism and pathology. In: Agarwal DP, Seitz HK (eds) Alcohol in health and disease. Dekker, New York, pp 87–102
Vaglenova J, Martinez SE, Porte S, Duester G, Farres J, Pares X (2003) Expression, localization and potential physiological significance of alcohol dehydrogenase in the gastrointestinal tract. Eur J Biochem 270:2652–2662
Yin S-J, Chou F-J, Chao S-F, Tsai S-F, Liao C-S, Wang S-L, Wu C-W, Lee S-C (1993) Alcohol and aldehyde dehydrogenases in human esophagus: comparison with the stomach enzyme activities. Alcohol Clin Exp Res 17:376–381
Lieber CS (2005) Metabolism of alcohol. Clin Liver Dis 9:1–35
Estonius M, Svensson S, Hoog J-O (1996) Alcohol dehydrogensde in human tissue: localisation of transcripts coding for five classes of the enzyme. FEBS Lett 397:338–342
Goedde HW, Agarwal DP (1987) Polymorphism of aldehyde dehydrogenase and alcohol sensitivity. Enzyme 37:29–44
Jelski W, Kozlowski M, Laudanski J, Niklinski J, Szmitkowski M (2009) The activity of Class I, II, III and IV alcohol dehydrogenase (ADH) isoenzymes and aldehyde dehydrogenase (ALDH) in esophageal cancer. Dig Dis Sci (in press)
Skursky L, Kovar J, Stachova M (1979) A sensitive assay for alcohol dehydrogenase activity in blood serum. Anal Biochem 89:65–71
Jelski W, Zalewski B, Chrostek L, Szmitkowski M (2004) The activity of class I, II, III and IV of alcohol dehydrogenase (ADH) isoenzymes and aldehyde dehydrogenase (ALDH) in the colorectal cancer. Dig Dis Sci 49:977–981
Wierzchowski J, Dafeldecker WP, Holmquist B, Vallee BL (1989) Fluorimetric assay for isozymes of human alcohol dehydrogenase. Anal Biochem 178:57–62
Jelski W, Chrostek L, Szmitkowski M, Laszewicz W (2002) The activity of class I, II, III and IV of alcohol dehydrogenase isoenzymes in gastric mucosa. Dig Dis Sci 47:1554–1557
Koivusalo M, Baumann M, Uotila L (1989) Evidence for the identity of glutathione-dependent formaldehyde dehydrogenase and class III alcohol dehydrogenase. FEBS Lett 257:105–109
Dohmen K, Baraona E, Ishibashi H, Pozzato G, Moretti M, Matsunaga C, Fujimoto K, Lieber CS (1996) Ethnic differences in gastric σ-alcohol dehydrogenase activity and ethanol first pass metabolism. Alcohol Clin Exp Res 20:1569–1576
Hou MF, Tsai LY, Tsai SM, Huang CJ, Huang YS, Hsieh JS, Chan HM, Wang JY, Chuang CH, Chen FM, Huang TJ (1999) Biochemical markers for assessment of bone metastases in patients with breast cancer. Kaohsiung J Med Sci 15:452–460
Jelski W, Zalewski B, Chrostek L, Szmitkowski M (2007) Alcohol dehydrogenase (ADH) isoenzymes and aldehyde dehydrogenase (ALDH) activity in the sera of patients with colorectal cancer. Clin Exp Med 7:154–157
Pestallozzi D, Buhler R, von Wartburg JP, Hess M (1983) Immunohistochemical localization of alcohol dehydrogenase in the human gastrointestinal tract. Gastroenterology 85:1011–1016
Yokoyama H, Baraona E, Lieber CS (1995) Upstream structure of human ADH 7 gene and the organ distribution of its expression. Biochem Biophys Res Commun 216:216–222
Jelski W, Chrostek L, Szmitkowski M (2007) The activity of class I, III and IV alcohol dehydrogenase isoenzymes and aldehyde dehydrogenase in gastric cancer. Dig Dis Sci 52:531–535
Jelski W, Chrostek L, Zalewski B, Szmitkowski M (2008) Alcohol dehydrogenase (ADH) isoenzymes and aldehyde dehydrogenase (ALDH) activity in the sera of patients with gastric cancer. Dig Dis Sci 53:2101–2105
Jelski W, Chrostek L, Szmitkowski M (2007) The activity of class I, II, III and IV alcohol dehydrogenase isoenzymes and aldehyde dehydrogenase in the pancreatic cancer. Pancreas 35:142–146
Jelski W, Zalewski B, Szmitkowski M (2008) Alcohol dehydrogenase (ADH) isoenzymes and aldehyde dehydrogenase (ALDH) activity in the sera of patients with pancreatic cancer. Dig Dis Sci 53:2276–2280
Chen YJ, Chen C, Wu DC, Lee CH, Wu CI, Lee JM, Goan YG, Huang SP, Lin CC, Li TC, Chou YP, Wu MT (2006) Interactive effects of lifetime alcohol consumption and alcohol and aldehyde dehydrogenase polymorphisms on esophageal cancer risks. Int J Cancer 119:2827–2831
Li DP, Dandara C, Walther G, Parker MI (2008) Genetic polymorphisms of alcohol metabolising enzymes: their role in susceptibility to oesophageal cancer. Clin Chem Lab Med 46:323–328
Terry MB, Gammon MD, Zhang FF, Vaughan TL, Chow WH, Risch HA, Schoenberg JB, Mayne ST, Stanford JL, West AB, Rotterdam H, Blot WJ, Fraumeni JF Jr, Santella RM (2007) Alcohol dehydrogenase 3 and risk of esophageal and gastric adenocarcinomas. Cancer Causes Control 18:1039–1046
Maly IP, Toranelli M, Sasse D (1999) Distribution of alcohol dehydrogenase isoenzymes in human liver acinus. Histochem Cell Biol 111:391–397
Seitz HK, Egerer G, Oneta C, Kramer S, Sieg A, Klee F, Simanowski UA (1996) Alcohol dehydrogenase in the human colon and rectum. Digestion 57:105–108
Chrostek L, Szmitkowski M, Wierzchowski J (1994) Activity of class I and II alcohol dehydrogenase in the sera of alcoholics. Eur J Clin Chem Clin Biochem 32:881–884
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Jelski, W., Kozlowski, M., Laudanski, J. et al. Alcohol dehydrogenase isoenzymes and aldehyde dehydrogenase activity in the sera of patients with esophageal cancer. Clin Exp Med 9, 131–137 (2009). https://doi.org/10.1007/s10238-008-0028-7
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DOI: https://doi.org/10.1007/s10238-008-0028-7