Interleukin-1β polymorphisms, Helicobacter pyloriinfection in individuals from Northern Brazil with gastric adenocarcinoma

Abstract.

Gastric carcinogenesis is a complex, multistep process, which may be influenced by many factors and is the second most common type of malignancy and the second most-common cause of mortality in the word. Interleukin-1 is up-regulated in the presence of Helicobacter pylori and is important for initiating and amplifying the inflamatory response to this infection. Recently interleukin-1 polymorphisms have been associated with the development of gastric adenocarcinoma. In this study we investigated the presence of H. pylori and host genotypes that are highly associated with gastric alterations. DNA samples were extracted and PCR-RFLP was utilized for genotyping IL-1B (-511) polymorphisms, PCR-VNTR was utilized for genotyping IL-1RN, and PCR-CTPP was utilized for genotyping IL-1B (-31), the presence of H. pylori was detected by the urease test. Our results indicate a correlation between H. pylori infection and the development of gastric cancer. We did not find an association between the presence of genotype T (thymine) in bases -511 and -31 and gastric adenocarcinoma. We also did not find any association between this polymorphism and specific type of tumor (diffuse type and intestinal type).