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Study of extracellular matrix in vocal fold biomechanics using a two-phase model

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Abstract

The extracellular matrix (ECM) of the vocal fold tissue consists primarily of fibrous and interstitial proteins. The purpose of this study was to investigate the effects of selective enzymatic digestion of two ECM proteins, namely elastin and versican, on the elasticity of rabbit vocal fold tissue. Quasi-static, sinusoidal, uniaxial tensile tests were performed. The data were analyzed within the framework of a model of the ECM as a two-phase composite material consisting of collagen fibrils as the reinforcing fibers and noncollagenous ECM proteins as the matrix. To validate the two-phase model, the regression parameters for the fibers’ volume fraction and shear modulus in a different animal model were compared with corresponding published data. The proposed model was then used to analyze rabbit vocal fold tissues. The mean value and the standard deviation of the fiber volume fraction were found to be 8.49 \(\pm \)3.75 % for the control samples (\(n\) = 4), 0.59 \(\pm \)1.13 % after elastin removal (\(n\) = 4), and 8.22 \(\pm \)1.06 % after versican removal (\(n\) = 4). The results suggest that elastin removal may lead to a reduction in tissue stiffness, through counteracting the reinforcement of collagen fibrils.

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Acknowledgments

This work was supported by National Institute of Health Grant Nos. R01-DC005788 (Luc Mongeau, principal investigator) and R01-DC004336 (Susan Thibeault, principal investigator).

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Authors and Affiliations

  1. Department of Mechanical Engineering, McGill University, 817 Sherbrooke Street West, Montreal, Quebec, H3A 0C3, Canada

    Amir K. Miri, Rosaire Mongrain & Luc Mongeau

  2. Department of Hearing and Speech Sciences, University of Maryland-College Park, College Park, MD, USA

    Nicole Y. K. Li

  3. Department of Mechanical Engineering and Applied Mechanics, University of Pennsylvania, Philadelphia, PA, USA

    Reza Avazmohammadi

  4. Division of Otolaryngology-Head and Neck Surgery, Department of Surgery, University of Wisconsin-Madison, Madison, WI, USA

    Susan L. Thibeault

Authors
  1. Amir K. Miri
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  2. Nicole Y. K. Li
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  3. Reza Avazmohammadi
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  4. Susan L. Thibeault
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  5. Rosaire Mongrain
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  6. Luc Mongeau
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Correspondence to Amir K. Miri.

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Miri, A.K., Li, N.Y.K., Avazmohammadi, R. et al. Study of extracellular matrix in vocal fold biomechanics using a two-phase model. Biomech Model Mechanobiol 14, 49–57 (2015). https://doi.org/10.1007/s10237-014-0585-5

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  • DOI: https://doi.org/10.1007/s10237-014-0585-5

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