Summary
Insect octopamine receptors are G-protein coupled receptors. They can be coupled to second messenger pathways to mediate either increases or decreases in intracellular cyclic AMP levels or the generation of intracellular calcium signals. Insect octopamine receptors were originally classified on the basis of second messenger changes induced in a variety of intact tissue preparations. Such a classification system is problematic if more than one receptor subtype is present in the same tissue preparation. Recent progress on the cloning and characterization in heterologous cell systems of octopamine receptors from Drosophila and other insects is reviewed. A new classification system for insect octopamine receptors into “α-adrenergic-like octopamine receptors (OctαRs)”, “β-adrenergic-like octopamine receptors (OctβRs)” and “octopamine/tyramine (or tyraminergic) receptors” is proposed based on their similarities in structure and in signalling properties with vertebrate adrenergic receptors. In future studies on the molecular basis of octopamine signalling in individual tissues it will be essential to identify the relative expression levels of the different classes of octopamine receptor present. In addition, it will be essential to identify if co-expression of such receptors in the same cells results in the formation of oligomeric receptors with specific emergent pharmacological and signalling properties.
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References
Alkema MJ, Hunter-Ensor M, Ringstad N, Horvitz HR (2005) Tyramine functions independently of octopamine in the Caenorhabditis elegans nervous system. Neuron 46:247–260
Arakawa S, Gocayne JD, McCombie WR, Urquhart DA, Hall LM, Fraser CM, Venter JC (1990) Cloning, localization, and permanent expression of a Drosophila octopamine receptor. Neuron 4:343–354
Balfanz S, Strünker T, Frings S, Baumann A (2005) A family of octapamine receptors that specifically induce cyclic AMP production or Ca2+ release in Drosophila melanogaster. J Neurochem 93:440–451
Bischof LJ, Enan EE (2004) Cloning, expression and functional analysis of an octopamine receptor from Periplaneta americana. Insect Biochem Mol. Biol. 34:511–521
Blenau W, Baumann A (2001) Molecular and pharmacological properties of insect biogenic amine receptors. Lessons from Drosophila melanogaster and Apis mellifera. Arch Insect Biochem Physiol 48:13–38
Blenau W, Balfanz S, Baumann A (2000) Am tyr1. Characterization of a gene from honeybee (Apis mellifera) brain encoding a functional tyramine receptor. J Neurochem 74:900–908
Brody T, Cravchik A. (2000) Drosophila melanogaster G protein-coupled receptors. J Cell Biol 150:F83–F88
Chang DJ, Li XC, Lee YS et al (2000) Activation of a heterologously expressed octopamine receptor coupled only to adenylyl cyclase produces all the features of presynaptic facilitation in Aplysia sensory neurons. Proc Natl Acad Sci USA 97:1829–1834
Cole SH, Carney GE, McClung CA, Willard SS, Taylor BJ, Hirsh J. (2005) Two functional but non-complementing Drosophila tyrosine decarboxylase genes: distinct roles for neural tyramine and octopamine in female fertility. J Biol Chem 280:14948–14955
David JC, Coulon JF (1985) Octopamine in invertebrates and vertebrates. A review. Prog Neurobiol 24:141–185
Davis RL (1996) Physiology and biochemistry of Drosophila learning mutants. Physiol Rev 76:299–317
Dudai Y, Zvi S (1984) High-affinity [3H] octopamine-binding sites in Drosophila melanogaster: interactions with ligands and relationship to octopamine receptors. Comp Biochem C 77:145–151
Eddy SR (1998) Profile hidden Markov models. Bioinformatics 14:755–763
Ehlert JE, Addison CA, Burdick MD, Kunkel SL, Strieter RM (2004) Identification and partial characterization of a variant of human CXCR3 generated by posttranscriptional exon skipping. J Immunology 173:6234–6240
Evans PD (1980) Biogenic amines in the insect nervous system. Adv Insect Physiol 15:317–473
Evans PD (1981) Multiple receptor types for octopamine in the locust. J Physiol (Lond) 318:99–122
Evans PD (1984a) Studies on the mode of action of octopamine, 5–hydroxytryptamine and proctolin on a myogenic rhythm in the locust. J Exp Biol 110:231–251
Evans PD (1984b) A modulatory octopaminergic neurone increases cyclic nucleotide levels in locust skeletal muscle. J Physiol (Lond) 348:307–324
Evans PD (1984c) The role of cyclic nucleotides and calcium in the mediation of the modulatory effects of octopamine on locust skeletal muscle. J Physiol (Lond) 348:325–340
Evans PD (1987) Phenyliminoimidazolidine derivatives activate both OCTOPAMINE1 and OCTOPAMINE2 receptor subtypes in locust skeletal muscle. J Exp Biol 129:239–250
Evans PD (1993) Molecular studies on insect octopamine receptors. In: Pichon Y (eds) Comparative molecular neurobiology. Birkhauser Verlag, Basel, pp 286–296
Evans PD, Robb S (1993) Octopamine receptor subtypes and their modes of action. Neurochem Res 18:869–874
Evans PD, Robb, S, Cheek TR, Reale V, Hannan, FL, Swales LS, Hall M, Midgley JM (1995) Agonist-specific coupling of G-protein coupled receptors to second messenger systems. Prog Brain Res 106:259–268
Grosmaitre X, Marion-Poll F, Renou M (2001) Mamestra brassicae putative octopamine receptor (OAR) mRNA, complete cds. Available from http://www.ncbi.nlm.nih.gov/entrez/query.fcgi.
Han KA, Millar NS, Davis RL (1998) A novel octopamine receptor with preferential expression in Drosophila mushroom bodies. J Neurosci 18:3650–3658
Hannan F, Hall LM (1996) Temporal and spatial expression patterns of two G-protein coupled receptors in Drosophila melanogaster. Invert Neurosci 2:71–83
Kenakin T (1995) Agonist-receptor efficacy II: agonist trafficking of receptor signals. Trends Pharmacol Sci 16:232–238
Kutsukake M, Komatsu A, Yamamoto D, Ishiwa-Chigusa S (2000) A tyramine receptor gene mutation causes a defective olfactory behaviour in Drosophila melanogaster. Gene 245:31–42
Lee HG, Seong CS, Kim YC, Davis RL, Han KA (2003) Octopamine receptor OAMB is required for ovulation in Drosophila melanogaster. Dev Biol 264:179–190
Maqueira B, Chatwin H, Evans PD (2004) Cloning and expression of a novel family of octopamine G-protein coupled receptors from Drosophila. Soc Neurosci Abst 30:274.8
Maqueira B, Chatwin H, Evans PD (2005) Identification and characterization of a novel family of Drosophila β-adrenergic-like octopamine G-protein coupled receptors. J Neurochem 94:547–560
Monastirioti M, Linn CE, White K (1996) Characterization of Drosophila β-hydroxylase gene and isolation of mutant flies lacking octopamine. J Neurosci 16:3900–3911
Nagaya Y, Kutsukake M, Chigusa SI, Komatsu A (2002) A trace amine, tyramine, functions as a neuromodulator in Drosophila melanogaster. Neurosci Lett 329:324–328
Ohata H, Utsumi T, Ozoe Y (2003) B96Bom encodes a Bombyx mori tyramine receptor negatively coupled to adenylate cyclase. Insect Mol Biol 12:217–223
Ono H, Yoshikawa H (2004) Identification of amine receptors from a swallowtail butterfly, Papilio xuthus L.: cloning and mRNA localization in foreleg chemosensory organ for recognition of host plants. Insect Biochem Mol Biol 34:1247–1256
Pierce KL, Premont RT, Lefkowitz RJ (2002) Seven-transmembrane receptors. Nat Rev Mol Cell Biol 3:639–650
Robb S, Cheek TR, Hannan FL, Hall LM, Midgley JM, Evans PD (1994) Agonist-specific coupling of a cloned Drosophila octopamine/tyramine receptor to multiple second messenger systems. EMBO J 13:1325–1330
Roeder T (1992) A new octopamine receptor class in locust nervous tissue, the octopamine 3 (OA3) receptor. Life Sci 50:21–28
Roeder T (1994) Biogenic amines and their receptors in insects. Comp Biochem Physiol 107C:1–12
Roeder T (1999) Octopamine in invertebrates. Prog. Neurobiol. 59:533–561
Roeder T (2005) Tyramine and octopamine: ruling behaviour and metabolism. Ann Rev Entomol 50:447–477
Roeder T, Nathanson JA (1993) Characterization of insect neuronal octopamine receptors (OA3 receptors). Neurochem Res 18:921–925
Roeder T, Degen J, Dyczkowski C, Gewecke M (1995) Pharmacology and molecular biology of octopamine receptors from different insect species. Prog Brain Res 106:249–258
Roeder T, Seifert M, Kahler C, Gewecke M (2003) Tyramine and octopamine: antagonistic modulators of behaviour and metabolism. Arch Insect Biochem Physiol 54:1–13
Saraswati S, Fox LE, Soll DR, Wu C-F (2003) Tyramine and octopamine have opposite effects on the locomotion of Drosophila larvae. J Neurobiol 58:425–441
Sarmiento JM, Anazco CC, Campos DM, Prado GN, Navarro J, Gonzalez CB (2004) Novel down-regulatory mechanism of the surface expression of the vasopressin V2 receptor by an alternative splice receptor variant. J Biol Chem 279:47017–47023
Saudou F, Amlaiky N, Plassat JL, Borrelli E, Hen R (1990) Cloning and characterization of a Drosophila tyramine receptor. EMBO J 9:3611–3617
Srivastava D, Yu E, Kennedy K, Chatwin H, Reale V, Hammon M, Smith T, Evans PD (2004) Steroids and catecholamines differentially activate a novel Drosophila G-protein coupled receptor. Soc Neurosci Abstr 30:395.1
Srivastava DP, Yu EJ, Kennedy K, Chatwin H, Reale V, Hamon M, Smith T, Evans PD (2005) Rapid, nongenomic responses to ecdysteroids and catecholamines mediated by a novel Drosophila G-protein-coupled receptor. J Neuroscience 25:6145–6155
The FlyBase Consortium (2003) The FlyBase database of the Drosophila genome projects and community literature. Nucleic Acids Res 31:172–175
Thummel CS, Chory J (2002) Steroid signalling in plants and insects—common themes, different pathways. Gen Dev 16:3113–3129
Tomaschko K-H (1999) Nongenomic effects of ecdysteroids. Arch Insect Biochem Physiol 41:89–98
Vanden Broeck J (2001) Insect G protein coupled receptors and signal transduction. Arch Insect Biochem Physiol 48:1–12
Vanden Broeck I, Vulsteke V, Huybrechts R, DeLoof A (1995) Characterization of a cloned locust tyramine receptor cDNA by functional expression in permanently transformed Drosophila S2 cells. J Neurochem 64:2387–2395
Von Nickisch-Rosenegk E, Krieger J, Kubick S, et al (1996) Cloning of biogenic amine receptors from moths (Bombyx mori and Heliothis virescens). Insect Biochem Mol Biol 26:817–827
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This work was supported by the BBSRC through The Babraham Institute. B. M. was additionally supported by the María L. de Sánchez Scholarship (Academia de Ciencias Físicas, Matemáticas y Naturales, Venezuela/Girton College, Cambridge, UK.).
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Evans, P.D., Maqueira, B. Insect octopamine receptors: a new classification scheme based on studies of cloned Drosophila G-protein coupled receptors. Invert Neurosci 5, 111–118 (2005). https://doi.org/10.1007/s10158-005-0001-z
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DOI: https://doi.org/10.1007/s10158-005-0001-z