Abstract
Neutrophils play a pivotal role in immunity against infection by ingesting and killing invading microbes. Neutrophils isolated from human peripheral blood have been used for a number of studies conducted for evaluation of immunomodulating drugs, cytokines, and microbe products. Human promyelocytic leukemia cells, HL-60, have been extensively studied because they can differentiate into neutrophil-like cells by addition of all-trans retinoic acid or dimethyl sulfoxide. For a system that would always allow experimental use of granulocytic cells in a uniformly activated state, we have established HL-60 cell lines with increased migratory activity by transducing the CXC chemokine receptor 1 (CXCR1) gene. When these cell lines were primed with CXC chemokine ligand 8 (IL-8), a slight increase in reactive oxygen species production induced by phorbol myristate acetate (PMA) or zymosan A stimuli was observed. A significance increase in migratory activity was noticed when the HL-60 cells transduced CXCR1 were stimulated with IL-8 in the Boyden chamber method. The gene-transduced HL-60 cell lines may be used as a substitute for neutrophils in screening the effects of various immunomodulating drugs on the migratory activity induced by IL-8.
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Acknowledgments
We thank Dr. T. Ubagai for the valuable discussions, and Ms. C. Miyazaki and Mrs. K. Sawada for their technical assistance. This work was supported in part by a grant-in-aid from the Ministry of Education, Culture, Sports, Science and Technology of Japan (21591300). The gene transformation experiments were conducted with the approval of the Teikyo University Living Modified Organisms Safety Committee (No. 1102120A1b).
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The authors have no financial conflict of interests to declare.
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Kikuchi-Ueda, T., Tansho, S. & Ono, Y. Enhancement of interleukin-8-induced chemotactic response and reactive oxygen species production in HL-60 cells expressing CXCR1. J Infect Chemother 18, 283–287 (2012). https://doi.org/10.1007/s10156-011-0321-3
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DOI: https://doi.org/10.1007/s10156-011-0321-3