Abstract
We have set up an ex vivo ovine ruminal model, which can mimic the multicellular process to explore the early steps in Salmonella typhimurium lipopolysaccharide (LPS) stimulation using RNA-seq technology. Ovine ruminal explants were collected for histological and transcriptional analysis and supernatants collected to quantitate lactate dehydrogenase (LDH) enzymes. A total of 8 and 523 genes were significantly over-expressed between LPS-treated and control tissues at 6 and 12 h, respectively. However, six and seven hundred and thirteen genes were substantially repressed between the aforementioned tissues, correspondingly. Key genes up-regulated in response to the addition of LPS were tumor necrosis factor (TNF), interlukin (IL)-1 beta(b), IL-6, IL-8, IL-17B, IL-19, MMP-1, MMP-3, and integrin alpha 2 (ITGA8, 9). This study shows for the first time that galectin-1 is up-regulated in an ex vivo ruminal segment model exposed to bacterial lipopolysaccharide following 6 h of incubation. The ruminal segment model has been shown to be a suitable tool to study the bacterial lipopolysaccharide effects on the ovine ruminal tissues prior to in vivo assessment.
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Acknowledgements
This research was supported by the Start-up Research Grant Program provided by Foshan University, Foshan city, Guangdong province for distinguished researchers, the National Natural Science Foundations of China (31272419), the National Major Development Program of Transgenic Breeding (2014ZX0800953B), the Program for the National High Technology Research and Development Program of China (863 Program 2013AA102503), and the Program for Changjiang Scholar and Innovation Research Team in University (IRT_15R62).
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Animal experiments were conducted in accordance with the guidelines of Beijing Municipality on the Review of Welfare and Ethics of Laboratory Animals approved by the Beijing Municipality Administration Office of Laboratory Animals (BAOLA), and under the protocol (CAU-AEC-2010–0603) approved by the China Agricultural University Animal Ethics Committee. All experimental procedures were also approved by the Institutional Animal Care and Committee of China Agricultural University (The certificate of Beijing Laboratory Animal employee, ID: 15883).
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Abouhajer, F., El-Ashram, S., Karama, M. et al. An ex vivo ruminal ovine model to study the immediate immune response in the context of bacterial lipopolysaccharide. Funct Integr Genomics 18, 277–285 (2018). https://doi.org/10.1007/s10142-018-0589-9
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DOI: https://doi.org/10.1007/s10142-018-0589-9