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Identification and Mapping of Amplified Fragment Length Polymorphism Markers Linked to Shell Color in Bay Scallop, Argopecten irradians irradians (Lamarck, 1819)

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Abstract

Amplified fragment length polymorphisms (AFLP) were used to study the inheritance of shell color in Argopecten irradians. Two scallops, one with orange and the other with white shells, were used as parents to produce four F1 families by selfing and outcrossing. Eighty-eight progeny, 37 orange and 51 white, were randomly selected from one of the families for segregation and mapping analysis with AFLP and microsatellite markers. Twenty-five AFLP primer pairs were screened, yielding 1138 fragments, among which 148 (13.0%) were polymorphic in two parents and segregated in progeny. Six AFLP markers showed significant (P < 0.05) association with shell color. All six loci were mapped to one linkage group. One of the markers, F1f335, is completely linked to the gene for orange shell, which we designated as Orange1, without any recombination in the progeny we sampled. The marker was amplified in the orange parent and all orange progeny, but absent in the white parent and all the white progeny. The close linkage between F1f335 and Orange1 was validated using bulk segregation analysis in two natural populations, and all our data indicate that F1f335 is specific for the shell color gene, Orange1. The genomic mapping of a shell color gene in bay scallop improves our understanding of shell color inheritance and may contribute to the breeding of molluscs with desired shell colors.

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Acknowledgments

We thank Qingdao Jinying Ocean Science & Technology Development Co., Ltd. for their assistance in scallop hatchery and farm operations. This study was funded by grants from the NSFC (30671622, 30500381), and also partly supported by grants from NSFC (39825121).

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Authors and Affiliations

  1. Institute of Oceanology, Chinese Academy of Sciences, Qingdao, 266071, China

    Yanjie Qin, Xiao Liu, Haibin Zhang & Guofan Zhang

  2. Graduate School, Chinese Academy of Sciences, Beijing, 100039, China

    Yanjie Qin

  3. Haskin Shellfish Research Laboratory, Institute of Marine and Coastal Sciences, Rutgers University, Port Norris, NJ, 08349, USA

    Ximing Guo

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  1. Yanjie Qin
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Correspondence to Xiao Liu.

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Qin, Y., Liu, X., Zhang, H. et al. Identification and Mapping of Amplified Fragment Length Polymorphism Markers Linked to Shell Color in Bay Scallop, Argopecten irradians irradians (Lamarck, 1819). Mar Biotechnol 9, 66–73 (2007). https://doi.org/10.1007/s10126-006-6076-7

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