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Molecular Identification and Expression of Differentially Regulated Genes of the European Flounder, Platichthys flesus, Submitted to Pesticide Exposure

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Abstract

Effects of pesticide exposure on the European flounder, Platichthys flesus, were investigated using a suppression subtractive hybridization method (SSH) to identify up- and down-regulated genes after a 30-day exposure to herbicides (a cocktail of atrazine, diuron, and isoproturon, and a single herbicide, glyphosate). A total of 256 expressed gene sequences were identified as having the potential for being differentially expressed, of which 116 could be identified by homology with databased sequences. The metabolic functions with which they are associated include energy production, general metabolism, signaling, transport, immune system, and structure. Expression of 14 of these genes was analyzed in liver, muscle, and gills by reverse transcriptase-polymerase chain reaction (RT-PCR) under experimental conditions (0, 15, and 30 days of exposure) and under field conditions (sampling in two estuaries displaying different levels of pesticide contamination). This study provides a first basis for studying the response of fish to pesticide exposure and allows the characterization of new potential genetic markers of pesticide contamination in the field.

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Acknowledgments

This research program was financially supported by the IAV institute. The authors are grateful to G. Durand (Pôle Analytique des Eaux) and V. Veron (IAV) for pesticide data, to the fishermen J. Gouyen (Ster), R.Triballier, and F. Triballier (Vilaine) for field sampling, and to B. Simon for real-time PCR assistance.

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Authors and Affiliations

  1. Laboratoire des Sciences de l'Environnement Marin (LEMAR), UMR-CNRS 6539, Institut Universitaire Européen de la Mer, Université de Bretagne Occidentale, 29280, Plouzané, France

    J. Marchand, G. Charrier, L. Quiniou & J. Laroche

  2. Laboratoire Adaptation et Diversité en Milieu Marin, UMR-CNRS 7127, 29682, Roscoff Cedex, France

    A. Tanguy

  3. Laboratoire de Biochimie, Université de Bretagne Occidentale, Faculté de Médecine CS 93837, 29238, Brest Cedex 3, France

    E. Plee-Gauthier

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  1. J. Marchand
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Appendices

Appendix 1

Appendix 1 Up-regulated genes identified in the SSH library of the ADI (atrazine/diuron/isoproturon) experiment after 30 days of exposure, with significant databases matches
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Appendix 2

Appendix 2 Down-regulated genes identified in the SSH library of the ADI (atrazine/diuron/isoproturon) experiment after 30 days of exposure, with significant database matches
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Appendix 3

Appendix 3 Up-regulated genes identified in the SSH library of the G (glyphosate) experiment after 30 days of exposure, with significant database matches
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Appendix 4

Appendix 4 Down-regulated genes identified in the SSH library of the G (glyphosate) experiment after 30 days of exposure, with significant database matches
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Marchand, J., Tanguy, A., Charrier, G. et al. Molecular Identification and Expression of Differentially Regulated Genes of the European Flounder, Platichthys flesus, Submitted to Pesticide Exposure. Mar Biotechnol 8, 275–294 (2006). https://doi.org/10.1007/s10126-005-0099-3

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