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Isolation of New Symbiodinium Strains from Tridacnid Giant Clam (Tridacna crocea) and Sea Slug (Pteraeolidia ianthina) Using Culture Medium Containing Giant Clam Tissue Homogenate

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Abstract

Recent molecular biological studies have revealed that some photosymbiotic invertebrates dwelling in coral reefs host several genetically different dinoflagellates, Symbiodinium species, as symbionts. However, little is known about the difference in physiologic characteristics among these symbionts living in a single host, because some Symbiodinium strains are difficult to culture in vitro. To isolate some of these Symbiodinium strains, we have developed an agar culture medium plate containing antibiotics and a giant clam tissue homogenate. Using-this medium we isolated two new Symbiodinium strains from two molluscan hosts, Tridacna crocea and Pteraeolidia ianthina, each of which hosted two different Symbiodinium strains belonging to Symbiodinium C and D, respectively. The tissue homogenate was essential for the growth of Symbiodinium D. Although it was not essential for the growth of Symbiodinium C, it did stimulate the initial growth. For the isolation of some Symbiodinium strains, isolation medium containing host homogenate is effective.

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Acknowledgments

Prof. S. Miyachi is acknowledged for his valuable comments on the manuscript. This work was performed as a part of The Industrial Science and Technology Project for Technological Development of Biological Resources in Bioconsortia supported by New Energy and Industrial Technology Development Organization (NEDO).

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Authors and Affiliations

  1. Marine Biotechnology Institute, Heita 3-75-1, Kamaishi, Iwate 026-0001, Japan

    Masaharu Ishikura, Kiyoshi Hagiwara, Kiyotaka Takishita, Miyuki Haga & Tadashi Maruyama

  2. Fisheries Experiment Station, Yaeyama Branch of Okinawa Prefectural Government Laboratory, Kabira 828-2, Ishigakishi, Okinawa 907-0453, Japan

    Kenji Iwai

  3. Japan Agency for Marine Earth Science and Technology, Marine Ecosystem Research Department, Natsushima 2-15, Yokosuka, Kauagawa 237-0061, Japan

    Tadashi Maruyama

  4. Yokosuka City Museum, Yokosuka, Kanagawa 238-0016, Japan

    Kiyoshi Hagiwara

Authors
  1. Masaharu Ishikura
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  2. Kiyoshi Hagiwara
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  3. Kiyotaka Takishita
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  4. Miyuki Haga
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  5. Kenji Iwai
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  6. Tadashi Maruyama
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Correspondence to Tadashi Maruyama.

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Ishikura, M., Hagiwara, K., Takishita, K. et al. Isolation of New Symbiodinium Strains from Tridacnid Giant Clam (Tridacna crocea) and Sea Slug (Pteraeolidia ianthina) Using Culture Medium Containing Giant Clam Tissue Homogenate. Mar. Biotechnol. 6, 378–385 (2004). https://doi.org/10.1007/s10126-004-1800-7

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  • DOI: https://doi.org/10.1007/s10126-004-1800-7

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