Abstract
Kluyveromyces marxianus is expected to be used in the production of yeast extracts due to its good fermentation ability and nutritional properties. Yeast autolysis is a key process to produce yeast extract and vacuum negative pressure stress can be used as an effective way to assist autolysis. However, the molecular mechanism of initiating Kluyveromyces marxianus autolysis induced by vacuum negative pressure and the higher temperature is still unclear. In this study, RNA-seq technology was performed mainly to analyze autolytic processes in Kluyveromyces marxianus strains. Considerable differentially expressed genes (DEGs) of downregulation were significantly enriched in 7 Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways related to synthesis and transport of RNA and ribosome, which indicated that abnormal protein translations had already occurred in autolytic process. Interestingly, due to obvious change of related DEGs, endoplasmic reticulum-associated degradation (ERAD) and autophagy were activated and cell wall integrity pathway was hindered. Under the continuous influence of the external stress environment, the long-term changes of the above pathways triggered a vicious circle of gradual damage to yeast cells, which is the main cause of yeast autolysis. These results may provide important clues for the in-depth interpretation of the yeast autolytic mechanism.
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Data availability
The datasets analyzed during the current study are available in the Sequence Read Archive (SRA) at NCBI with accession number SRP320 756.
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The authors thank the research team for their sincere cooperation and selfless dedication.
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This study was funded by the National Key R&D Program of China (Grant No. 2021YFC2101100).
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Xiao Li, Xiang-Ling Shen, Kun Zou, and Ze-Tao Xiao designed and directed research; Xiao Li, Ze-Tao Xiao, Ya-Li Tan, Jian-Hua Li, Yu-Di Luo, Xiao-Long Zhang, Han Ye, Yu-Peng Pei, Shuai-Jing Wang, Xue-Na Feng, and Qing-Yu Wang performed research and analyzed the data; and Xiao Li and Ze-Tao Xiao wrote the paper. All authors reviewed the manuscript.
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Li, X., Xiao, ZT., Tan, YL. et al. Transcriptomic analysis reveals process of autolysis of Kluyveromyces marxianus in vacuum negative pressure and the higher temperature. Int Microbiol 25, 515–529 (2022). https://doi.org/10.1007/s10123-022-00240-1
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DOI: https://doi.org/10.1007/s10123-022-00240-1