Abstract
Burkholderia cepacia complex (Bcc) members have clinical relevance as opportunistic pathogens in patients with cystic fibrosis and are responsible of numerous nosocomial infections. These closely related bacteria are also reported as frequent contaminants of industrial products. In this retrospective study, we use PCR and recA gene sequence analysis to identify at species level Bcc isolates recovered from massive consumption products and industrial processes in Argentina during the last 25 years. The sequences obtained were also compared with recA sequences from clinical Bcc isolates deposited in GenBank database. We detected Bcc in purified water and preserved products from pharmaceutics, cosmetics, household cleaning articles, and beverages industries. B. contaminans (which is prevalent among people with cystic fibrosis in Argentina) was the most frequent Bcc species identified (42% of the Bcc isolates studied). B. cepacia (10%), B. cenocepacia (5%), B. vietnamiensis (16%), B. arboris (3%), and the recently defined B. aenigmatica (24%) were also detected. Rec A sequences from all B. cepacia and most B. contaminans industrial isolates obtained in this study displayed 100% identity with recA sequences from isolates infecting Argentinean patients. This information brings evidence for considering industrial massive consumption products as a potential source of Bcc infections. In addition, identification at species level in industrial microbiological laboratories is necessary for a better epidemiological surveillance. Particularly in Argentina, more studies are required in order to reveal the role of these products in the acquisition of B. contaminans infections.
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Acknowledgments
Authors are grateful to University of Buenos Aires for funding this research. Special thanks are provided to microbiologists from different industries who provided isolates for this study. This article is dedicated to the memory of Prof. Miguel D’Aquino, who was S.T. and J.D. mentor, and promoter of this line of research.
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RecA sequences from industrial isolates obtained during this study are deposited in GenBank database (accession numbers are detailed in Table 1).
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This research has been funded by the University of Buenos Aires, School of Pharmacy and Biochemistry through the Account “Microbial Methods”, UBACyT 20020100100773 and UBACyT 200201001004484.
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ALDV performed research, (obtaining and maintenance of isolates, PCR, bioinformatics), analyzed data, and wrote the paper. AI, JLP, and CH performed research (MALDI TOF studies) and analyzed data. LI and MV performed research (PCR) and analyzed data. NB and TH performed research (maintenance of isolates, PCR, bioinformatics). ST conceived and designed study, performed research (obtaining isolates). JD conceived and designed study, performed research (obtaining isolates, PCR, bioinformatics), analyzed data, and wrote the paper. All authors reviewed and corrected the manuscript.
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De Volder, A.L., Teves, S., Isasmendi, A. et al. Distribution of Burkholderia cepacia complex species isolated from industrial processes and contaminated products in Argentina. Int Microbiol 24, 157–167 (2021). https://doi.org/10.1007/s10123-020-00151-z
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DOI: https://doi.org/10.1007/s10123-020-00151-z