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Decreasing the Cut-off Score Value of MALDI-ToF MS Increase the Identities of Burkholderia cepacia Complex Species

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Abstract

Burkholderia cepacia complex (Bcc) comprises 24 related species genetically distinct, associated with high mortality in cystic fibrosis (CF) patients. Due to a high level of similarity among Bcc species, accurate identification has been problematic, and most conventional and automated phenotypic tests have shown low accuracy. We evaluated accuracy of MALDI-ToF MS decreasing the cut-off score value to distinguish Bcc species compared to recA gene sequencing. A total of 145 Bcc isolates were analyzed. B. vietnamiensis (41.37%), B. cenocepacia IIIA (23.44%), B. multivorans (20%), B. cenocepacia IIIB (11.03%), and B. contaminans (2.75%) among other species were identified by recA sequencing. MALDI-ToF MS identified 100% of Bcc isolates at the genus level and 53.1% at the species level. By decreasing cut-off values for ≥1.70, the correct identification at the species level increased to 74.5%. MALDI-ToF MS proved to be useful at the genus level identification, but it still requires improvements that allow more precise identification, requiring continuous updates and addition of new spectra to its database. A review of interpretative criteria is a field to be explored with a large collection of Bcc species.

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Funding

This study was financed by the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq). Grant No. E-26/010.001040/2015.

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Authors and Affiliations

  1. Departamento de Microbiologia, Imunologia e Parasitologia, Faculdade de Ciências Médicas, Universidade do Estado do Rio de Janeiro, Rio de Janeiro, Brazil

    Edgard de Freitas Vianna, Ludimila Santos da Silva Pentagna, Nicoli Izzy Miotto Menezes, Flávia Alvim Dutra de Freitas, Cassiana da Costa Ferreira Leite, Robson Souza Leão & Elizabeth Andrade Marques

  2. Departamento de Bioquímica, Instituto de Biologia Roberto Alcântara Gomes, Universidade do Estado do Rio de Janeiro, Rio de Janeiro, Brazil

    Rodolpho Mattos Albano

Authors
  1. Edgard de Freitas Vianna
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  2. Ludimila Santos da Silva Pentagna
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  3. Nicoli Izzy Miotto Menezes
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  4. Flávia Alvim Dutra de Freitas
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  5. Cassiana da Costa Ferreira Leite
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  6. Rodolpho Mattos Albano
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  7. Robson Souza Leão
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  8. Elizabeth Andrade Marques
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Contributions

EFV, EAM, RSL, RMA Proposed the research. EFV, NIMM, LSSP, FADF, Prepared samples and performed experiments. EFV, EAM, CCFL, designed the experiments. EFV, FADF, RMA performed bio informatics analysis. EFV, Wrote manuscript and prepared figures. EAM, supervised the project. All authors reviewed the manuscript.

Corresponding author

Correspondence to Elizabeth Andrade Marques.

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The authors declare that they have no conflict of interest.

Ethical Approval

This study was approved by the Research Ethics Committee of our centre (University Hospital Pedro Ernesto, State University of Rio de Janeiro, Brazil), CAAE 16427019.8.0000.5259 (ref. 3.460.239).

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Vianna, E.F., Pentagna, L.S.S., Menezes, N.I.M. et al. Decreasing the Cut-off Score Value of MALDI-ToF MS Increase the Identities of Burkholderia cepacia Complex Species. Curr Microbiol 78, 2259–2263 (2021). https://doi.org/10.1007/s00284-021-02493-x

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  • DOI: https://doi.org/10.1007/s00284-021-02493-x

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