Skip to main content
SpringerLink
Log in

Fluorescent detection of Cu(II) by chitosan-based AIE bioconjugate

  • Papers
  • Published:
Chinese Journal of Polymer Science Aims and scope Submit manuscript

Abstract

Detection of Cu(II) is very important in disease diagnose, biological system detection and environmental monitoring. Previously, we found that the product TPE-CS prepared by attaching the chromophores of tetraphenylethylene (TPE) to the chitosan (CS) chains showed excellent fluorescent properties. In this study, we tried to use TPE-CS for detecting Cu(II) because of the stable complexation of CS with heavy metals and the luminosity mechanism of the Restriction of Intramolecular Rotations (RIR) for aggregation-induced emission (AIE)-active materials. The fluorescence intensity changed when TPE-CS was contacted with different metal ions, to be specific, no change for Na+, slightly increase for Hg2+, Pb2+, Zn2+, Cd2+, Fe2+, Fe3+ due to the RIR caused by the complexation between CS and metal ions. However, for Cu2+, an obvious fluorescence decrease was observed because of the Photoinduced-Electron-Transfer (PET). Moreover, we found that the quenched FL intensity of TPE-CS was proportional to the concentration of Cu(II) in the range of 5 μmol/L to 100 μmol/L, which provided a new way to quantitatively detect Cu(II). Besides, TPE-CS has excellent water-solubility as well as absorbability (the percentage of removal, R = 84%), which is an excellent detection probe and remover for Cu(II).

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Wang, F.Y., Li, Y.X., Li, W.Y., Chen, J., Zhang, Q.F., Shahzad, S.A. and Yu, C., Talanta, 2015, 132: 72

    Article  CAS  Google Scholar 

  2. Georgopoulos, P.G., Roy, A., Yonone, L.M.J., Opiekun, R.E. and Lioy, P.J.J., Toxicol. Environ. Health B: Crit. Rev., 2001, 4(4): 341

    Article  CAS  Google Scholar 

  3. Bonham, M., O'Connor, J.M., Hannigan, B.M. and Strain, J.J., Brit. J. Nutr., 2007, 87(5): 393

    Article  Google Scholar 

  4. Sanji, T., Nakamura, M. and Tanaka, M., Tetrahedron Lett., 2011, 52(26): 3283

    Article  CAS  Google Scholar 

  5. Zietz, B.P., de Vergara, J.D. and Dunkelberg, H., Environ. Res., 2003, 92(2): 129

    Article  CAS  Google Scholar 

  6. Kumbhar, H.S., Yadav, U.N., Gadilohar, B.L. and Shankarling, G.S., Sensor Actuat. B: Chem., 2014, 203: 174

    Article  CAS  Google Scholar 

  7. Kandasamy, K., Ganesabaskaran, S., Pachamuthu, M.P. and Ramanathan, A., Spectrochim. Acta A, 2015, 148: 184

    Article  CAS  Google Scholar 

  8. Gonzales, A.P., Firmino, M.A., Nomura, C.S., Rocha, F.R., Oliveira, P.V. and Gaubeur, I., Anal. Chim. Acta, 2009, 636(2): 198

    Article  CAS  Google Scholar 

  9. Becker, J.S., Zoriy, M.V., Pickhardt, C., Palomero, G.N. and Zilles, K., Anal. Chem., 2005, 77(10): 3208

    Article  CAS  Google Scholar 

  10. Chen, X.L., Zeng, W.F., Yang, X.D., Lu, X.W., Qu, J.Q. and Liu, R.Y., Chinese J. Polym. Sci., 2016, 34(3): 324

    Article  CAS  Google Scholar 

  11. Cotruvo, J.A., Jr., Aron, A.T., Ramos, T.K.M. and Chang, C.J., Chem. Soc. Rev., 2015, 44(13): 4400

  12. Domaille, D.W., Que, E.L. and Chang, C.J., Nat. Chem. Biol., 2008, 4(3): 168

    Article  CAS  Google Scholar 

  13. Yuan, W.Z., Lu, P., Chen, S.M., Lam, J.W.Y., Wang, Z.M., Liu, Y., Kwok, H.S., Ma, Y.G. and Tang, B.Z., Adv. Mater., 2010, 22(19): 2159

    Article  CAS  Google Scholar 

  14. Hong, Y.N., Chen, S.J., Leung, C.W.T., Lam, J.W.Y., Liu, J.Z., Tseng, N.W., Kwok, R.T.K., Yu, Y., Wang, Z.K. and Tang, B.Z., ACS Appl. Mater. Inter., 2011, 3(9): 3411

    Article  CAS  Google Scholar 

  15. Feng, H.T., Song, S., Chen, Y.C., Shen, C.H. and Zheng, Y.S., J. Mater. Chem. C, 2014, 2(13): 2353

    Article  CAS  Google Scholar 

  16. Nie, J.Y., Wang, Z.K., Zhang, K. and Hu, Q.L., RSC Adv., 2015, 5(47): 37346

    Article  CAS  Google Scholar 

  17. Nie, J.Y., Wang, Z.K., Zhang, J.Z., Yang, L., Pang, Y.C. and Hu, Q.L., RSC Adv., 2015, 5(83): 68243

    Article  CAS  Google Scholar 

  18. Huang, X.F., Jia, J.W., Wang, Z.K. and Hu, Q.L., Chinese J. Polym. Sci., 2014, 33(2): 284

    Article  Google Scholar 

  19. Zhang, K., Zhuang, P.Y., Wang, Z.K., Li, Y.L., Jiang, Z.Q., Hu, Q.L., Liu, M.Y. and Zhao, Q.X., Carbohydr. Polym., 2012, 90(4): 1515

    Article  CAS  Google Scholar 

  20. Nie, J.Y., Lu, W.T., Ma, J.J., Yang, L., Wang, Z.K., Qin, A. and Hu, Q.L., Sci. Rep., 2015, 5: 7635

    Article  CAS  Google Scholar 

  21. Fang, J.D., Zhang, K., Jia, J.W., Wang, Z.K. and Hu, Q.L., RSC Adv., 2015, 5(120): 99418

    Article  CAS  Google Scholar 

  22. Wang, Z.K. and Hu, Q.L., Biomed. Mater., 2010, 5(4): 045007

    Article  Google Scholar 

  23. Wang, Z.K., Hu, Q.L. and Wang, Y.X., Sci. China Chem., 2011, 54(2): 380

    Article  CAS  Google Scholar 

  24. Huang, X.F., Sun, Y.F., Nie, J.Y., Lu, W.T., Yang, L., Zhang, Z.L., Yin, H.P., Wang, Z.K. and Hu, Q.L., Int. J. Biol. Macromol., 2015, 75: 322

    Article  CAS  Google Scholar 

  25. Ke, J.H., Wang, Z.K., Li, Y.Z. and Hu, Q.L., Chinese J. Polym. Sci., 2012, 30(3): 436

    Article  CAS  Google Scholar 

  26. Wang, Z.K., Hu, Q.L. and Cai, L., Chinese J. Polym. Sci., 2010, 28(5): 801

    Article  CAS  Google Scholar 

  27. Zhang, K., Zhao, M., Cai, L., Wang, Z.K., Sun, Y.F. and Hu, Q.L., Chinese J. Polym. Sci., 2010, 28(4): 555

    Article  CAS  Google Scholar 

  28. Lee, S.J., Lee, S.S., Lah, M.S., Hong, J.M. and Jung, J.H., Chem. Commun., 2006, (43): 4539

    Article  Google Scholar 

  29. Wang, Z.K., Chen, S.J., Lam, J.W.Y., Qin, W., Kwok, R.T., Xie, N., Hu, Q. and Tang, B.Z., J. Am. Chem. Soc., 2013, 135(22): 8238

    Article  CAS  Google Scholar 

  30. Jia, J.W., Wang, Z.K., Lu, W.T., Yang, L., Wu, Q.W., Qin, W., Hu, Q.L. and Tang, B.Z., J. Mater. Chem. B, 2014, 2: 8406

    Article  CAS  Google Scholar 

  31. Wang, Z.K., Liu, Y.L., Jia, J.W., Chen, S.J., Qin, W., Hu, Q.L. and Tang, B.Z., J. Mater. Chem. B, 2016, 4: 5265

    Article  CAS  Google Scholar 

  32. Wang, Z.K., Nie, J.Y., Qin, W., Hu, Q.L. and Tang, B.Z., Nat. Commun., 2016, 7: 12033

    Article  CAS  Google Scholar 

  33. Li, M., Hong, Y.N., Wang, Z.K., Chen, S.J., Gao, M., Kwok, R.T.K., Qin, W., Lam, J.W.Y., Zheng, Q.C. and Tang, B.Z., Macromol. Rapid Commun., 2013, 34: 767

    Article  CAS  Google Scholar 

  34. Hong, Y.N., Lam, J.W.Y. and Tang, B.Z., Chem. Commun., 2009 (29): 4332

    Article  Google Scholar 

  35. Huang, H., Shi, F.P., Li, Y.N., Niu, L., Gao, Y., Shah, S.M. and Su, X.G., Sensor Actuat. B: Chem., 2013, 178: 532

    Article  CAS  Google Scholar 

  36. Xu, G.Y., Wang, J.F., Si, G.F., Wang, M.H., Xue, X., Wu, B.X. and Zhou, S.S., Sensor Actuat. B: Chem., 2016, 230: 684

    Article  CAS  Google Scholar 

  37. Li, Z.A., Lou, X.D., Yu, H.B., Li, Z. and Qin, J.G., Macromolecules, 2008, 41(20): 7433

    Article  CAS  Google Scholar 

  38. Zhang, S.R., Wang, Q., Tian, G.H. and Ge, H.G., Mater. Lett., 2014, 115: 233

    Article  CAS  Google Scholar 

  39. Wang, X., Chen, Q.Y., Yin, Z.L., Hu, H.P. and Xiao, Z.L., J. Cent. South. Univ. Technol., 2011, 18: 48

    Article  CAS  Google Scholar 

  40. Wu, Z.C., Wang, Z.Z., Liu, J., Yin, J.H. and Kuang, S.P., Colloid. Surface. A, 2016, 499: 141

    Article  CAS  Google Scholar 

  41. Azzam, E.M., Eshaq, G., Rabie, A.M., Bakr, A.A., Abd, E. A.A., El Metwally, A.E. and Tawfik, S.M., Int. J. Biol. Macromol., 2016, 89: 507

    Article  CAS  Google Scholar 

  42. Cao, W.X., Lin, G.L., Jin, L.S. and Wei, Z.H., Liaoning Chemical Industry (in Chinese), 2007, 36(8): 529

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Ministry of Education Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou, 310027, China

    Ya-lan Liu, Zheng-ke Wang  (王征科) & Qiao-ling Hu  (胡巧玲)

  2. Key Laboratory of Adsorption and Separation Materials & Technologies of Zhejiang Province, Hangzhou, 310027, China

    Ya-lan Liu, Zheng-ke Wang  (王征科) & Qiao-ling Hu  (胡巧玲)

  3. Department of Chemistry, Hong Kong Branch of Chinese National Engineering Research Center for Tissue Restoration and Reconstruction, Hong Kong University of Science and Technology, Clear Water Bay, Hong Kong, China

    Wei Qin & Ben Zhong Tang  (唐本忠)

Authors
  1. Ya-lan Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Zheng-ke Wang  (王征科)
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Wei Qin
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Qiao-ling Hu  (胡巧玲)
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Ben Zhong Tang  (唐本忠)
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to Zheng-ke Wang  (王征科), Qiao-ling Hu  (胡巧玲) or Ben Zhong Tang  (唐本忠).

Additional information

This work was financially supported by the Key Science Technology Innovation Team of Zhejiang Province (No. 2013TD02) and the National Natural Science Foundation of China (Nos. 21104067, 21274127, 21374099 and 51473144).

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Liu, Yl., Wang, Zk., Qin, W. et al. Fluorescent detection of Cu(II) by chitosan-based AIE bioconjugate. Chin J Polym Sci 35, 365–371 (2017). https://doi.org/10.1007/s10118-017-1876-y

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10118-017-1876-y

Keywords

Search

Navigation