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A novel chitosan-based sponge coated with self-assembled thrombin/tannic acid multilayer films as a hemostatic dressing

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Abstract

In order to prepare a novel hemostatic dressing for uncontrolled hemorrhage, a porous chitosan sponge was coated with self-assembled (thrombin/tannic acid) n films, which were based on hydrogen bonding interactions between thrombin and tannic acid at physiologic pH. According to the whole blood clotting test, the coated chitosan sponges showed a significantly high rate of blood clotting due to the addition of thrombin. On the other hand, the storable half-life of immobilized thrombin is extended to 66.9 days at room temperature, which is 8.5 times longer than unfixed thrombin. It is because of the immobilization effect of, not only the porous structure of chitosan sponge but also the interactions between thrombin and tannic acid. In addition, the tannic acid has similar antibacterial effect to chitosan. Therefore, it is an excellent combination of chitosan, thrombin and tannic acid. Besides, all of materials in this research have been approved by the United States Food and Drug Administration (FDA). So the chitosan-based sponge is a promising candidate dressing for uncontrolled hemorrhage due to its storable, bio-safe and highly effective hemostatic properties.

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Authors and Affiliations

  1. MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou, 310027, China

    Xiao-fei Huang, Jing-wei Jia, Zheng-ke Wang  (王征科) & Qiao-ling Hu  (胡巧玲)

Authors
  1. Xiao-fei Huang
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  2. Jing-wei Jia
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  3. Zheng-ke Wang  (王征科)
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  4. Qiao-ling Hu  (胡巧玲)
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Corresponding authors

Correspondence to Zheng-ke Wang  (王征科) or Qiao-ling Hu  (胡巧玲).

Additional information

This work was financially supported by the National Natural Science Foundation of China (Nos. 21104067, 21274127 and 21374099 and 51473144), Key Basic Research Development Plan (973 Program) of China (Nos. 2009CB930104 and 2011CB606203), Fundamental Research Funds for the Central Universities (No. 2013QNA4048) and Technology Foundation for Selected Overseas Scholar of Zhejiang Province.

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Huang, Xf., Jia, Jw., Wang, Zk. et al. A novel chitosan-based sponge coated with self-assembled thrombin/tannic acid multilayer films as a hemostatic dressing. Chin J Polym Sci 33, 284–290 (2015). https://doi.org/10.1007/s10118-015-1580-8

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  • DOI: https://doi.org/10.1007/s10118-015-1580-8

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