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Low-level laser therapy (LLLT) in sites grafted with osteoconductive bone substitutes improves osseointegration

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Abstract

The aim of this study was to evaluate the osseointegration of implants placed in areas grafted with different osteoconductive bone substitutes irradiated with infrared low-level laser therapy (LLLT). Fifty-six rats were randomly allocated into 4 groups: DBB, bone defects filled with deproteinized bovine bone graft (DBB); HA/TCP, bone defects filled with biphasic ceramic made of hydroxyapatite and β-tricalcium phosphate (HA/TCP); DBB-L, bone defects filled with DBB and treated by LLLT; HA/TCP-L, bone defects filled with HA/TCP and treated by LLLT. Bone defects were performed in the tibia of each animal and filled with the different biomaterials. The grafted areas were treated with LLLT (λ 808 nm, 100 mW, ϕ ∼ 0.60 mm) in 7 sessions with 48 h between the irradiations. After the 60-day period, the implants were placed, and the animals were euthanized after 15 and 45 days. The osseointegration and bone repair in the grafted area were evaluated by biomechanical, microtomographic and histometric analyses, and the expression of some bone biomarkers was evaluated by immunohistochemistry analysis. LLLT induced higher degree of osseointegration, which was associated with the greater expression of BMP2 and OCN. LLLT performed in areas grafted with osteoconductive bone substitutes prior to implant placement improves osseointegration.

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Acknowledgements

The authors would like to thank the laboratory technicians Leandro Alves dos Santos and Ana Claudia Gregolin Costa Miranda for their technical support during the histological analysis. This study was financed by the Brazilian agencies FAPESP (Fundação de Amparo a pesquisa do Estado de São Paulo, 2013/24318-0) and CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnológico, 459984/2014-4).

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Authors and Affiliations

  1. Department of Periodontology, Dental School, Universidade Federal de Uberlândia (UFU), Pará Av, 1720, Uberlândia, MG, 38400-902, Brazil

    Guilherme José Pimentel Lopes de Oliveira

  2. Department of Diagnosis and Surgery, School of Dentistry Campus of Araraquara, São Paulo State University (UNESP), Humaitá St. 1680, Araraquara, 14801-903, Brazil

    Maurício Andres Tinajero Aroni, Felipe Eduardo Pinotti, Elcio Marcantonio Jr & Rosemary Adriana Chiérici Marcantonio

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  1. Guilherme José Pimentel Lopes de Oliveira
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Correspondence to Guilherme José Pimentel Lopes de Oliveira.

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de Oliveira, G.J.P.L., Aroni, M.A.T., Pinotti, F.E. et al. Low-level laser therapy (LLLT) in sites grafted with osteoconductive bone substitutes improves osseointegration. Lasers Med Sci 35, 1519–1529 (2020). https://doi.org/10.1007/s10103-019-02943-w

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