Abstract
Curcumin and salicylic acid are both phenolic compounds and they can both affect cancer treatment efficacy. In this study, the effects of methylene blue-curcumin (CU-MB) and methylene blue-salicylic acid (SA-MB) ion pair complexes on MDA-MB-231 human breast cancer cells are studied. According to the thermodynamic parameters, the stability of curcumin and salicylic acid complexes ion pair complexes was compared. The free energy of ion pair interactions was calculated based on binding constants. A comparison of the free energies of the complexes (CU-MB: ∆G°b1 = − 21.11 kJ/mol and ∆G°b2 = − 8.37 kJ/mol, SA-MB: ∆G°b1 = − 12.92 kJ/mol and ∆G°b2 = − 9.02 kJ/mol) indicates that the interaction of methylene blue in first binding interaction with curcumin is greater than that of methylene blue with salicylic acid. Electrostatic interactions are the main forces in the binding of both compounds to methylene blue. All forces are inter-molecular physical interactions. The results of cellular experiments show that ion pairing has enhanced the reduction of cell viability. By increasing molecular stability and prevention of dimerization of methylene blue, the cell killing potential of methylene blue increases and it subsequently causes enhancement of photodynamic efficacy.
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References
Sarveiya V, Templeton JF, Benson H a E (2004) Ion-pairs of ibuprofen: increased membrane diffusion. J Pharm Pharmacol 56:717–724. https://doi.org/10.1211/0022357023448
Sarveiya V, Templeton JF, Benson HAE (2005) Effect of lipophilic counter-ions on membrane diffusion of benzydamine. Eur J Pharm Sci 26:39–46. https://doi.org/10.1016/j.ejps.2005.04.013
Song IS, Choi MK, Shim WS, Shim CK (2013) Transport of organic cationic drugs: effect of ion-pair formation with bile salts on the biliary excretion and pharmacokinetics. Pharmacol Ther 138:142–154. https://doi.org/10.1016/j.pharmthera.2013.01.006
Elshaer A, Hanson P, Mohammed AR (2014) A novel concentration dependent amino acid ion pair strategy to mediate drug permeation using indomethacin as a model insoluble drug. Eur J Pharm Sci 62:124–131. https://doi.org/10.1016/j.ejps.2014.05.022
Wang M, Fang L, Ren C, Li T (2008) Effect of ion-pairing and enhancers on scutellarin skin permeability. J Pharm Pharmacol 60:429–435. https://doi.org/10.1211/jpp.60.4.0004
Nam SH, Xu YJ, Nam H et al (2011) Ion pairs of risedronate for transdermal delivery and enhanced permeation rate on hairless mouse skin. Int J Pharm 419:114–120. https://doi.org/10.1016/j.ijpharm.2011.07.027
Lozoya-Agullo I, González-Álvarez I, González-Álvarez M et al (2016) Development of an ion-pair to improve the colon permeability of a low permeability drug: atenolol. Eur J Pharm Sci 93:334–340. https://doi.org/10.1016/j.ejps.2016.08.035
Hui M, Quan P, Yang Y, Fang L (2014) The effect of ion-pair formation combined with penetration enhancers on the skin permeation of loxoprofen. Drug Deliv 7544:1–8. https://doi.org/10.3109/10717544.2014.981768
Griesser J, Hetényi G, Moser M et al (2017) Hydrophobic ion pairing: key to highly payloaded self-emulsifying peptide drug delivery systems. Int J Pharm 520:267–274. https://doi.org/10.1016/j.ijpharm.2017.02.019
Jiang Y, Fang L, Niu X et al (2008) The effect of ion pairing on the skin permeation of amlodipine. Pharmazie 63:356–360. https://doi.org/10.1691/ph.2008.7302
Fini A, Bassini G, Monastero A, Cavallari C (2012) Diclofenac salts, VIII. Effect of the counterions on the permeation through porcine membrane from aqueous saturated solutions. Pharmaceutics 4:413–429. https://doi.org/10.3390/pharmaceutics4030413
Suresh P, Paul S (2011) Ion-paired drug delivery: an avenue for bioavailability improvement. Sierra Leone J Biomed Res 3:70–76. https://doi.org/10.4314/sljbr.v3i2.71806
Megwa SA, Cross SE, Whitehouse MW et al (2000) Effect of ion pairing with alkylamines on the in-vitro dermal penetration and local tissue disposition of salicylates. J Pharm Pharmacol 52:929–940. https://doi.org/10.1211/0022357001774813
Zhang JY, Fang L, Tan Z et al (2009) Influence of ion-pairing and chemical enhancers on the transdermal delivery of meloxicam Enhanced penetration of meloxicam. Drug Dev Ind Pharm 35:663–670. https://doi.org/10.1080/03639040802578111
Neubert R (1989) Ion pair transport across membranes. Pharm Res 6:743–747
Megwa SA, Cross SE, Benson HA, Roberts MS (2000) Ion-pair formation as a strategy to enhance topical delivery of salicylic acid. J Pharm Pharmacol 52:919–928
Tardivo JP, Del Giglio A, De Oliveira CS et al (2005) Methylene blue in photodynamic therapy: from basic mechanisms to clinical applications. Photodiagn Photodyn Ther 2:175–191. https://doi.org/10.1016/S1572-1000(05)00097-9
Vejselova D, Kutlu HM (2015) Inhibitory effects of salicylic acid on A549 human lung adenocarcinoma cell viability. Turkish J Biol 39:1–5. https://doi.org/10.3906/biy-1401-7
Khorsandi K, Hosseinzadeh R, Fateh M (2015) Curcumin intercalated layered double hydroxide nanohybrid as a potential drug delivery system for effective photodynamic therapy in human breast cancer cells. RSC Adv 5. https://doi.org/10.1039/c5ra15888e
Hosseinzadeh R, Khorsandi K (2017) Methylene blue, curcumin and ion pairing nanoparticles effects on photodynamic therapy of MDA-MB-231 breast cancer cell. Photodiagn Photodyn Ther 18:284–294. https://doi.org/10.1016/j.pdpdt.2017.03.005
Hosseinzadeh R, Khorsandi K, Jahanshiri M (2017) Combination photodynamic therapy of human breast cancer using salicylic acid and methylene blue. Spectrochim Acta - Part A Mol Biomol Spectrosc 184:198–203. https://doi.org/10.1016/j.saa.2017.05.008
Malvezzi A, do Amaral AT (2010) Ion pair stabilization effects on a series of procaine structural analogs. Eur J Pharm Sci 41:631–635. https://doi.org/10.1016/j.ejps.2010.09.003
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Khorsandi, K., Chamani, E., Hosseinzadeh, G. et al. Comparative study of photodynamic activity of methylene blue in the presence of salicylic acid and curcumin phenolic compounds on human breast cancer. Lasers Med Sci 34, 239–246 (2019). https://doi.org/10.1007/s10103-018-2571-0
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DOI: https://doi.org/10.1007/s10103-018-2571-0