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Assessment of the use of LED phototherapy on bone defects grafted with hydroxyapatite on rats with iron-deficiency anemia and nonanemic: a Raman spectroscopy analysis

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Abstract

This study aimed to assess bone repair in defects grafted or not with hydroxyapatite (HA) on healthy and iron-deficiency anemia (IDA) rats submitted or not to LED phototherapy (LED-PT) by Raman spectroscopy. The animals were divided in eight groups with five rats each: Clot; Clot + LED; IDA + Clot; IDA + LED; Graft; Graft + LED; IDA + Graft; and IDA + Graft + LED. When appropriated, irradiation with IR LED (λ850 ± 10 nm, 150 mW, CW, Φ = 0.5 cm2, 16 J/cm2, 15 days) was carried out. Raman shifts: ∼960 [symmetric PO4 stretching (phosphate apatite)], ∼1,070 [symmetric CO3 stretching (B-type carbonate apatite)], and ∼1,454 cm−1 [CH2/CH3 bending in organics (protein)] were analyzed. The mean peak values for ∼960, ∼1,070, and ∼1,454 cm−1 were nonsignificantly different on healthy or anemic rats. The group IDA + Graft + LED showed the lowest mean values for the peak ∼960 cm−1 when compared with the irradiated IDA group or not (p ≤ 0.001; p ≤ 0.001). The association of LED-PT and HA-graft showed lowest mean peak at ∼1,454 cm−1 for the IDA rats. The results of this study indicated higher HA peaks as well as a decrease in the level of organic components on healthy animals when graft and LED phototherapy are associated. In the other hand, IDA condition interfered in the graft incorporation to the bone as LED phototherapy only improved bone repair when graft was not used.

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Acknowledgments

We would like to thank the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) for providing financial support for this project.

Conflict of interest

The authors received a grant from the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), a government research agency, but have full control of all primary data and agree to allow the journal to review their data if requested.

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Authors and Affiliations

  1. Center of Biophotonics, School of Dentistry, Federal University of Bahia, Av. Araújo Pinho, 62, Canela, Salvador, BA, CEP 40110-150, Brazil

    Isabele Cardoso Vieira de Castro, Cristiane Becher Rosa, João Alves dos Reis Júnior, Luiz Gaudêncio Passos Moreira, Juliana S. Aragão, Artur Felipe dos Santos Barbosa & Antonio L. B. Pinheiro

  2. School of Dentistry, Federal University of Bahia, Salvador, BA, Brazil, 40110-150

    Luiz Gaudêncio Passos Moreira & Juliana S. Aragão

  3. University of Camilo Castelo Branco Núcleo do Parque Tecnológico de São José dos Campos, Rod. Presidente Dutra Km 139, Eugênio de Melo, São José dos Campos, SP, 12247-004, Brazil

    Landulfo Silveira Jr & Antonio L. B. Pinheiro

  4. National Institute of Optics and Photonics, University of São Paulo, Physics Institute of São Carlos, São Carlos, SP, Brazil, 13560-970

    Antonio L. B. Pinheiro

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  1. Isabele Cardoso Vieira de Castro
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  8. Antonio L. B. Pinheiro
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Correspondence to Antonio L. B. Pinheiro.

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de Castro, I.C.V., Rosa, C.B., dos Reis Júnior, J.A. et al. Assessment of the use of LED phototherapy on bone defects grafted with hydroxyapatite on rats with iron-deficiency anemia and nonanemic: a Raman spectroscopy analysis. Lasers Med Sci 29, 1607–1615 (2014). https://doi.org/10.1007/s10103-014-1562-z

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  • DOI: https://doi.org/10.1007/s10103-014-1562-z

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