Skip to main content

Advertisement

SpringerLink
Log in

The role of transforming growth factor β1 in fractional laser resurfacing with a carbon dioxide laser

  • Original Article
  • Published:
Lasers in Medical Science Aims and scope Submit manuscript

Abstract

The aim of this study was to investigate the role of transforming growth factor β1 in mechanisms of cutaneous remodeling induced by fractional carbon dioxide laser treatment. The dorsal skin of Kunming mice was exposed to a single-pass fractional CO2 laser treatment. Biopsies were taken at 1 h and at 1, 3, 7, 14, 21, 28, and 56 days after treatment. Transforming growth factor (TGF) β1 expression in skin samples was evaluated by ELISA, dermal thickness by hematoxylin–eosin staining, collagen and elastic fibers by Ponceau S and Victoria blue double staining, and types I and III collagens by ELISA. The level of TGF β1 in the laser-treated areas of skin was significantly increased compared with that in the control areas on days 1 (p < 0.05), 3 (p < 0.01), and 7 (p < 0.05) and then decreased by day 14 after treatment, at which time it had returned to the baseline level. Dermal thickness and the amount of type I collagen of the skin of the laser-treated areas had increased significantly (p < 0.05) compared with that in control areas on days 28 and 56. Fibroblast proliferation showed a positive correlation with TGF β1 expression during the early stages (r = 0.789, p < 0.01), and there was a negative correlation between the level of TGF β1 and type I collagen in the late stages, after laser treatment (r = −0.546, p < 0.05). TGF β1 appears to be an important factor in fractional laser resurfacing.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

References

  1. Manstein D, Herron GS, Sink RK, Tanner H, Anderson RR (2004) Fractional photothermolysis: a new concept for cutaneous remodeling using microscopic patterns of thermal injury. Lasers Surg Med 34(5):426–438

    Article  PubMed  Google Scholar 

  2. Hantash BM, Bedi VP, Sudireddy V, Struck SK, Herron GS, Chan KF (2006) Laser-induced transepidermal elimination of dermal content by fractional photothermolysis. J Biomed Opt 11(4):041115

    Article  PubMed  Google Scholar 

  3. Hantash BM, Bedi VP, Chan KF, Zachary CB (2007) Ex vivo histological characterization of a novel ablative fractional resurfacing device. Lasers Surg Med 39(2):87–95

    Article  PubMed  Google Scholar 

  4. Tierney EP, Hanke CW, Petersen J (2012) Ablative fractionated CO2 laser treatment of photoaging: a clinical and histologic study. Dermatol Surg 38(11):1777–1789

    Article  CAS  PubMed  Google Scholar 

  5. Tierney EP, Hanke CW (2011) Fractionated carbon dioxide laser treatment of photoaging: prospective study in 45 patients and review of the literature. Dermatol Surg 37(9):1279–1290

    Article  CAS  PubMed  Google Scholar 

  6. Alexiades-Armenaka M, Sarnoff D, Gotkin R, Sadick N (2011) Multi-center clinical study and review of fractional ablative CO2 laser resurfacing for the treatment of rhytides, photoaging, scars and striae. J Drugs Dermatol 10(4):352–362

    PubMed  Google Scholar 

  7. Barrientos S, Stojadinovic O, Golinko MS, Brem H, Tomic-Canic M (2008) Growth factors and cytokines in wound healing. Wound Repair Regen 16(5):585–601

    Article  PubMed  Google Scholar 

  8. Kopecki Z, Luchetti MM, Adams DH, Strudwick X, Mantamadiotis T, Stoppacciaro A, Gabrielli A, Ramsay RG, Cowin AJ (2007) Collagen loss and impaired wound healing is associated with c-Myb deficiency. J Pathol 211(3):351–361

    Article  CAS  PubMed  Google Scholar 

  9. Kane CJ, Hebda PA, Mansbridge JN, Hanawalt PC (1991) Direct evidence for spatial and temporal regulation of transforming growth factor beta 1 expression during cutaneous wound healing. J Cell Physiol 148(1):157–173

    Article  CAS  PubMed  Google Scholar 

  10. Riedel K, Riedel F, Goessler UR, Germann G, Sauerbier M (2007) TGF-beta antisense therapy increases angiogenic potential in human keratinocytes in vitro. Arch Med Res 38(1):45–51

    Article  CAS  PubMed  Google Scholar 

  11. Jiang X, Ge H, Zhou C, Chai X, Ren QS (2012) The role of vascular endothelial growth factor in fractional laser resurfacing with the carbon dioxide laser. Lasers Med Sci 27(3):599–606

    Article  PubMed  Google Scholar 

  12. Oue T, Puri P (1999) Abnormalities of elastin and elastic fibers in infantile hypertrophic pyloric stenosis. Pediatr Surg Int 15(8):540–542

    Article  CAS  PubMed  Google Scholar 

  13. Bogdan Allemann I, Kaufman J (2010) Fractional photothermolysis—an update. Lasers Med Sci 25(1):137–144

    Article  PubMed  Google Scholar 

  14. Baum CL, Arpey CJ (2005) Normal cutaneous wound healing: clinical correlation with cellular and molecular events. Dermatol Surg 31(6):674–686

    Article  CAS  PubMed  Google Scholar 

  15. Lee HS, Kooshesh F, Sauder DN, Kondo S (1997) Modulation of TGF-beta 1 production from human keratinocytes by UVB. Exp Dermatol 6(2):105–110

    Article  CAS  PubMed  Google Scholar 

  16. Wu L, Yu YL, Galiano RD, Roth SI, Mustoe TA (1997) Macrophage colony-stimulating factor accelerates wound healing and upregulates TGF-beta1 mRNA levels through tissue macrophages. J Surg Res 72(2):162–169

    Article  CAS  PubMed  Google Scholar 

  17. Rolfe KJ, Richardson J, Vigor C, Irvine LM, Grobbelaar AO, Linge C (2007) A role for TGF-beta1-induced cellular responses during wound healing of the non-scarring early human fetus? J Invest Dermatol 127(11):2656–2667

    Article  CAS  PubMed  Google Scholar 

  18. Liu W, Chua C, Wu X, Wang D, Ying D, Cui L, Cao Y (2005) Inhibiting scar formation in rat wounds by adenovirus-mediated overexpression of truncated TGF-beta receptor II. Plast Reconstr Surg 115(3):860–870

    Article  CAS  PubMed  Google Scholar 

  19. Clark RAF (1996) In: Clark RAF (ed) The molecular and cellular biology of wound repair, 2nd edn. Plenum, New York

    Google Scholar 

  20. Mazzieri R, Jurukovski V, Obata H, Sung J, Platt A, Annes E, Karaman-Jurukovska N, Gleizes PE, Rifkin DB (2005) Expression of truncated latent TGF-beta-binding protein modulates TGF-beta signaling. J Cell Sci 118:2177–2187

    Article  CAS  PubMed  Google Scholar 

  21. Sellheyer K, Bickenbach JR, Rothnagel JA, Bundman D, Longley MA, Krieg T, Roche NS, Roberts AB, Roop DR (1993) Inhibition of skin development by overexpression of transforming growth factor beta 1 in the epidermis of transgenic mice. Proc Natl Acad Sci U S A 90(11):5237–5241

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  22. Zambruno G, Marchisio PC, Marconi A, Vaschieri C, Melchiori A, Giannetti A, De Luca M (1995) Transforming growth factor-beta 1 modulates beta 1 and beta 5 integrin receptors and induces the de novo expression of the alpha v beta 6 heterodimer in normal human keratinocytes: implications for wound healing. J Cell Biol 129(3):853–865

    Article  CAS  PubMed  Google Scholar 

  23. Böttinger EP, Letterio JJ, Roberts AB (1997) Biology of TGF-beta in knockout and transgenic mouse models. Kidney Int 51(5):1355–1360

    Article  PubMed  Google Scholar 

  24. White LA, Mitchell TI, Brinckerhoff CE (2000) Transforming growth factor beta inhibitory element in the rabbit matrix metalloproteinase-1 (collagenase-1) gene functions as a repressor of constitutive transcription. Biochim Biophys Acta 1490(3):259–268

    Article  CAS  PubMed  Google Scholar 

  25. Papakonstantinou E, Aletras AJ, Roth M, Tamm M, Karakiulakis G (2003) Hypoxia modulates the effects of transforming growth factor-beta isoforms on matrix-formation by primary human lung fibroblasts. Cytokine 24:25–35

    Article  CAS  PubMed  Google Scholar 

  26. Yang L, Chan T, Demare J, Iwashina T, Ghahary A, Scott PG, Tredget EE (2001) Healing of burn wounds in transgenic mice overexpressing transforming growth factor-beta 1 in the epidermis. Am J Pathol 159(6):2147–2157

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  27. Adzick NS, Lorenz HP (1994) Cells, matrix, growth factors, and the surgeon. The biology of scarless fetal wound repair. Ann Surg 220(1):10–18

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  28. Silveira PC, Silva LA, Freitas TP, Latini A, Pinho RA (2011) Effects of low-power laser irradiation (LPLI) at different wavelengths and doses on oxidative stress and fibrogenesis parameters in an animal model of wound healing. Lasers Med Sci 26(1):125–131

    Article  PubMed  Google Scholar 

  29. Hakki SS, Bozkurt SB (2012) Effects of different setting of diode laser on the mRNA expression of growth factors and type I collagen of human gingival fibroblasts. Lasers Med Sci 27(2):325–331

    Article  PubMed  Google Scholar 

  30. Huang JS, Wang YH, Ling TY, Chuang SS, Johnson FE, Huang SS (2002) Synthetic TGF-beta antagonist accelerates wound healing and reduces scarring. FASEB J 16(10):1269–1270

    CAS  PubMed  Google Scholar 

  31. Fujimura T, Takema Y, Moriwaki S, Tsukahara K, Imokawa G, Yamada A, Imayama S (2001) Analytical method to examine the effects of carbon dioxide lasers on skin: a study using wrinkles induced in hairless mice. Lasers Surg Med 28(4):348–354

    Article  CAS  PubMed  Google Scholar 

  32. Nowak KC, McCormack M, Koch RJ (2000) The effect of superpulsed carbon dioxide laser energy on keloid and normal dermal fibroblast secretion of growth factors: a serum-free study. Plast Reconstr Surg 105(6):2039–2048

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgments

We are grateful to Yuxiu Liu for her invaluable support with the aspects of histology in this study. The research was supported by the Chinese Postdoctoral Science Foundation (2013M531166), Postdoctoral Science Foundation of Shanghai Jiao Tong University (13X100030001), and Biomedical Engineering Foundation of Shanghai Jiao Tong University (Yu2011MS70).

Author information

Authors and Affiliations

  1. Institute for Laser Medicine and Bio-Photonics, Department of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, 200240, China

    Xia Jiang, Chuanqing Zhou & Xinyu Chai

  2. Institute of Dermatology and Department of Dermatology at No. 1 Hospital, Anhui Medical University, Hefei, Anhui, 230032, China

    Hongmei Ge

  3. The Sixth People Hospital of Shanghai, Shanghai Jiao Tong University, Shanghai, 200233, China

    Hui Deng

Authors
  1. Xia Jiang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Hongmei Ge
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Chuanqing Zhou
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Xinyu Chai
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Hui Deng
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Hui Deng.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Jiang, X., Ge, H., Zhou, C. et al. The role of transforming growth factor β1 in fractional laser resurfacing with a carbon dioxide laser. Lasers Med Sci 29, 681–687 (2014). https://doi.org/10.1007/s10103-013-1383-5

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10103-013-1383-5

Keywords

Search

Navigation