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Low-level laser therapy enhances the expression of osteogenic factors during bone repair in rats

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Abstract

The aim of this study was to evaluate the effects of low-level laser therapy (LLLT) on bone formation, immunoexpression of osteogenic factors, and biomechanical properties in a tibial bone defect model in rats. Sixty male Wistar rats were distributed into bone defect control group (CG) and laser irradiated group (LG). Animals were euthanized on days 15, 30, and 45 post-injury. The histological and morphometric analysis showed that the treated animals presented no inflammatory infiltrate and a better tissue organization at 15 and 30 days postsurgery. Also, a higher amount of newly formed bone was observed at 15 days postsurgery. No statistically significant difference was observed in cyclooxygenase-2 immunoexpression among the groups at 15, 30, and 45 days in the immunohistochemical analysis. Considering RUNX-2, the immunoexpression was statistically higher in the LG compared to the CG at 45 days. BMP-9 immunoexpression was significantly higher in the LG in comparison to CG at day 30. However, there was no expressivity for this immunomarker, both in the CG and LG, at the day 45 postsurgery. No statistically significant difference was observed in the receptor activator of nuclear factor kappa-B ligand immunoexpression among the groups in all periods evaluated. No statistically significant difference among the groups was observed in the maximal load in any period of time. Our findings indicate that laser therapy improved bone healing by accelerating the development of newly formed bone and activating the osteogenic factors on tibial defects, but the biomechanical properties in LG were not improved.

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Acknowledgments

We thank the Brazilian funding agencies Fapesp and CNPq for the financial support of this research.

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Authors and Affiliations

  1. Department of Physiotherapy, Federal University of São Carlos, Rod Washington Luis, Km 235, São Carlos, São Paulo, Brazil

    Carla Roberta Tim & Nivaldo Antonio Parizotto

  2. Department of Morphology and Pathology, Federal University of São Carlos, Rod Washington Luis, Km 235, São Carlos, São Paulo, Brazil

    Karina Nogueira Zambone Pinto

  3. Departments of Biomechanics and Rehabilitation, University of São Paulo, Av. Bandeirantes, 3.900, Ribeirão Preto, São Paulo, Brazil

    Bruno Rafael Orsini Rossi

  4. Department of Bioscience, Federal University of São Paulo, Av. Ana Costa, 95, Vila Mathias, Santos, 11050-240, São Paulo, Brazil

    Kelly Fernandes & Ana Claudia Muniz Rennó

  5. Department of Oral Maxillofacial Surgery, School of Dentistry, University of the Sacred Heart, R. Irmã Arminda, 10-50, Bauru, São Paulo, Brazil

    Mariza Akemi Matsumoto

Authors
  1. Carla Roberta Tim
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  2. Karina Nogueira Zambone Pinto
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  3. Bruno Rafael Orsini Rossi
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  4. Kelly Fernandes
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  5. Mariza Akemi Matsumoto
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  6. Nivaldo Antonio Parizotto
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  7. Ana Claudia Muniz Rennó
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Correspondence to Ana Claudia Muniz Rennó.

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Tim, C.R., Pinto, K.N.Z., Rossi, B.R.O. et al. Low-level laser therapy enhances the expression of osteogenic factors during bone repair in rats. Lasers Med Sci 29, 147–156 (2014). https://doi.org/10.1007/s10103-013-1302-9

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  • DOI: https://doi.org/10.1007/s10103-013-1302-9

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