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The effect of noncoherent red light irradiation on proliferation and osteogenic differentiation of bone marrow mesenchymal stem cells

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Abstract

Mesenchymal stem cells (MSCs) are promising for use in regenerative medicine. Low-level light irradiation (LLLI) has been shown to modulate various processes in different biological systems. The aim of our study was to investigate the effect of red light emitted from a light-emitting diode (LED) on bone marrow MSCs with or without osteogenic supplements. MSCs both with and without osteogenic supplements were divided into four groups, and each group was irradiated at doses of 0, 1, 2 and 4 J/cm2. Cellular proliferation was evaluated using WST-8 and 5-ethynyl-2′-deoxyuridine (EdU) fluorescence staining. The alkaline phosphatase activity, mineralization, and expression of osteoblast master genes (Col1α1, Alpl, Bglap and Runx2) were monitored as indicators of MSC differentiation towards osteoblasts. In groups without osteogenic supplements, red light at all doses significantly stimulated cellular proliferation, whereas the osteogenic phenotype of the MSCs was not enhanced. In groups with osteogenic supplements, red light increased alkaline phosphatase activity and mineralized nodule formation, and stimulated the expression of Bglap and Runx2, but decreased cellular proliferation. In conclusion, nonconherent red light can promote proliferation but cannot induce osteogenic differentiation of MSCs in normal media, while it enhances osteogenic differentiation and decreases proliferation of MSCs in media with osteogenic supplements.

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Acknowledgments

The authors thank Chao Chen for his technical assistance. This work was supported by the National Natural Science Foundation of China (contract grant 50477043).

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Authors and Affiliations

  1. Department of Orthopedics, Renmin Hospital of Wuhan University, Wuhan, 430060, China

    Fei Peng

  2. Department of Orthopedics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, 1095 Jiefang Street, Wuhan, 430030, China

    Hua Wu, Xiqiang Xu & Jizhe Yu

  3. Department of Orthopedics, Minzu Hospital of Guangxi Zhuang Autonomous Region, Nanning, 530001, China

    Yadong Zheng

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  1. Fei Peng
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  2. Hua Wu
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  3. Yadong Zheng
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  5. Jizhe Yu
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Correspondence to Hua Wu.

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Peng, F., Wu, H., Zheng, Y. et al. The effect of noncoherent red light irradiation on proliferation and osteogenic differentiation of bone marrow mesenchymal stem cells. Lasers Med Sci 27, 645–653 (2012). https://doi.org/10.1007/s10103-011-1005-z

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  • DOI: https://doi.org/10.1007/s10103-011-1005-z

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