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Red light of 647 nm enhances osteogenic differentiation in mesenchymal stem cells

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An Erratum to this article was published on 07 April 2009

Abstract

The use of light for medical treatment has been studied previously. In this study, we examined the effect of light from a red light-emitting diode on osteogenic differentiation of mouse mesenchymal stem cells (D1 cells) which were cultured in the presence of osteogenic differentiation medium (ODM) for 3 days, then exposed to a red light-emitting diode (LED) light of 647 nm wavelength once for 10 s, 30 s or 90 s with radiation energies of 0.093 J, 0.279 J and 0.836 J, respectively. D1 cells in the presence of ODM differentiated into osteoblasts, and this process was enhanced on exposure to LED light in ODM medium. This effect was confirmed by increased Alizarin red staining, higher alkaline phosphatase (ALP) activity, higher mRNA expressions of osteocalcin, collagen type I, osteopontin and Runt-related transcription factor2 (Runx2), and higher levels by reverse transcriptase-polymerase chain reaction (RT-PCR) and by increased immunofluorescence staining against cluster of differentiation 44 (CD44) by immunofluorescence microscopy, confocal microscopy and flow cytometric analysis. These data suggest that osteogenic differentiation of mesenchymal stem cells (MSCs) in ODM is enhanced by LED light exposure.

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Acknowledgment

This work was supported by grant no. RTI04–03–03 from the Regional Technology Innovation Program of the Ministry of Commerce, Industry and Energy (MOCIE).

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Authors and Affiliations

  1. Department Orthopaedics, Chonnam National University Hwasun Hospital, 160 Ilsimri, Hwasun-eup, Hwasun-gun, Jeonnam, South Korea

    Hyung Keun Kim, Ji Hyun Kim, Azlina Amir Abbas, Jae Yoon Chung, Eun Kyoo Song & Taek Rim Yoon

  2. Cardiovascular Research Institute of Chonnam National University, Gwangju, South Jeolla, South Korea

    Hyung Keun Kim, Ji Hyun Kim & Taek Rim Yoon

  3. Department Electric Engineering, Chonnam National University, Gwangju, South Jeolla, South Korea

    Dong-Ok Kim & Sung-Jun Park

  4. Brain Korea 21 Project, Biomedical Human Resources Center, Chonnam National University, Gwangju, South Jeolla, 501–757, South Korea

    Taek Rim Yoon

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  1. Hyung Keun Kim
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Correspondence to Taek Rim Yoon.

Additional information

An erratum to this article can be found at http://dx.doi.org/10.1007/s10103-009-0669-0

An erratum to this article is available at http://dx.doi.org/10.1007/s10103-009-0669-0.

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Kim, H.K., Kim, J.H., Abbas, A.A. et al. Red light of 647 nm enhances osteogenic differentiation in mesenchymal stem cells. Lasers Med Sci 24, 214–222 (2009). https://doi.org/10.1007/s10103-008-0550-6

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  • DOI: https://doi.org/10.1007/s10103-008-0550-6

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