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Treatment of textile effluent using bacteria-immobilized graphene oxide nanocomposites: evaluation of effluent detoxification using Bellamya bengalensis

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Abstract

Recent studies have reported graphene-based nanomaterials as novel scaffolds for the development of vigorous biocatalytic systems. The present study investigated polyacrylic acid-linked graphene oxide (GO)–gelatin nanocomposite for the immobilization of moderately halotolerant engineered bacterial consortium consisting of Dietzia sp., Bacillus sp. and Pseudomonas mendocina. This biocatalyst was subsequently applied for treatment of hypersaline textile effluents collected from local textile manufacturing and processing units. Effluent treatment efficiency of this biocatalyst was assessed in terms of its dye, surfactant and salt-removal abilities from collected effluents. High metabolic activity recorded in the case of immobilized bacterial cells indicated that immobilization had stimulated improved growth as well as electrolyte and pH tolerance in bacterial cells. Examination of the treated effluents suggested approximately 99% removal of COD, color (dyes), electrolytes and surfactant. Probable cyto-genotoxic potential and oxidative stress inducing the ability of both untreated and treated effluents was determined with Bellamya bengalensis (fresh water snail). Comet formation in hepatopancreatic cells of snails exposed to untreated effluent was significantly higher than in individuals exposed to treated effluents which in turn were similar to organisms treated as control. Hence, results of this study indicated efficient performance of GO-based biocatalyst in augmenting biodegradation and detoxification of textile effluent. The low cost incurred during the synthesis and application of bacteria-immobilized GO nanocomposite and its reusability potential determined herein established the process of effluent treatment reported in this study as a promising approach for commercial wastewater treatment.

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Acknowledgements

Authors acknowledge all members of Department of Environmental Science, University of Calcutta, Kolkata, Department of Chemical Engineering, Jadavpur University, Kolkata and CSIR-Indian Institute of Chemical Biology, Kolkata for their constant support and suggestions. The ICP-OES used in this study was funded by the UGC-UPE grant and maintained by Department of Environmental Science, University of Calcutta. Authors are thankful to Center for Research in Nanoscience and Nanotechnology, University of Calcutta for characterization facilities like SEM, TEM and FTIR analysis. Authors are also thankful to Department of Polymer Science & Technology, University of Calcutta for providing the XRD facility.

Funding

This research did not receive any specific grant from funding agencies in the public, commercial, or not-for-profit sectors. This research was funded by departmental laboratory grants.

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Author notes

  1. Papita Das

    Present address: Department of Chemical Engineering, Jadavpur University, 188, Raja S.C. Mullick Road, Kolkata, West Bengal, 700032, India

Authors and Affiliations

  1. Department of Environmental Science, University of Calcutta, 35, Ballygunge Circular Road, Kolkata, West Bengal, 700019, India

    Priya Banerjee, Shramana Roy Barman & Aniruddha Mukhopadhyay

  2. CSIR-Indian Institute of Chemical Biology, 4 Raja S.C. Mullik Road, Kolkata, West Bengal, 700032, India

    Snehasikta Swarnakar

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  1. Priya Banerjee
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Correspondence to Papita Das.

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Banerjee, P., Barman, S.R., Swarnakar, S. et al. Treatment of textile effluent using bacteria-immobilized graphene oxide nanocomposites: evaluation of effluent detoxification using Bellamya bengalensis. Clean Techn Environ Policy 20, 2287–2298 (2018). https://doi.org/10.1007/s10098-018-1602-7

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