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Optimization of growth determinants of a potent cellulolytic bacterium isolated from lignocellulosic biomass for enhancing biogas production

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Abstract

Anaerobic digestion of lignocellulosic biomass is limited by inefficient hydrolysis of recalcitrant substrates, leading to low biogas yields where bacteria can be utilized successfully. In this study, we have chosen ten cellulose-degrading bacteria from active anaerobic slurry identified as Enterobacter ludwigii, Klebsiella pneumoniae, Pantoea agglomerans, Bacillus subtilis, Bacillus pumilus, Bacillus anthracis, Pseudomonas sp., Enterobacteriaceae bacterium Staphylococcus warneri, and Bacillus safensis; among them, E. ludwigii was found to be the most potent having an endoglucanase gene in the genome. The growth conditions of E. ludwigii were further optimized using Response Surface Methodology that designated 35 °C temperature, 6.5 pH, 5 % carboxymethylcellulose, 5 % yeast extract, 1 % ammonium nitrate as optimum growth conditions. The optimized growth module found to accelerate cellulase production at both transcription and translation level that in turn enhanced biogas production inside anaerobic digester as well. Finally, the growth-cellulase production relationship could be helpful in efficient industrial applications.

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Acknowledgments

Sincere thanks are due to DST (DST/SEED/INDO-UK/002/2011/VBU) and UGC, Govt. of India for financial support and research fellowships to RG, AKC, and SM. Thanks to Dr. S.N. Banerjee for valuable suggestions. Thanks are due to CRNN, University of Calcutta, India for SEM studies.

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    Authors and Affiliations

    1. Department of Environmental Studies, Visva-Bharati University, Santiniketan, 731 235, India

      Ramansu Goswami, Amit Kumar Chakraborty, Srinivasan Balachandran & Shibani Chaudhury

    2. Department of Zoology, Visva-Bharati University, Santiniketan, 731 235, India

      Suprabhat Mukherjee & Santi P. Sinha Babu

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    1. Ramansu Goswami
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    5. Santi P. Sinha Babu
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    6. Shibani Chaudhury
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    Correspondence to Shibani Chaudhury.

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    Ramansu Goswami and Suprabhat Mukherjee have contributed equally to this work.

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    Goswami, R., Mukherjee, S., Chakraborty, A.K. et al. Optimization of growth determinants of a potent cellulolytic bacterium isolated from lignocellulosic biomass for enhancing biogas production. Clean Techn Environ Policy 18, 1565–1583 (2016). https://doi.org/10.1007/s10098-016-1141-z

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