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Metagenomic next-generation sequencing restores the diagnosis of a rare infectious complication of B cell depletion

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Abstract

A 45-year-old female patient receiving rituximab for B cell non-Hodgkin follicular lymphoma presented unexplained recurrent fever, abdominal discomfort, and pollakiuria. We performed shotgun metagenomic sequencing from peri-kidney collection that identified a co-infection with Mycoplasma hominis and Ureaplasma urealyticum. The patient recovered with sequelae after appropriate antibiotic treatment was given.

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Author information

Authors and Affiliations

  1. Department of Clinical Immunopathology, Hôpital Saint-Louis, Assistance Publique Hôpitaux de Paris, 1, Avenue Claude Vellefaux, 75010, Paris, France

    Margaux Garzaro, Lin-Pierre Zhao, Marion Malphettes & Lionel Galicier

  2. Université de Paris, Paris, France

    Margaux Garzaro & Jean-David Bouaziz

  3. Department of Infectious Diseases, Hôpital Saint-Louis, Assistance Publique Hôpitaux de Paris, Paris, France

    Nathalie De Castro

  4. Department of Microbiology, Hôpital Saint-Louis, Assistance Publique Hôpitaux de Paris, Paris, France

    Séverine Mercier-Delarue, Francois Camelena, Béatrice Berçot, Jérôme Le Goff & Maud Salmona

  5. Bacteriology Department, Associated Laboratory of the French National Center for Bacterial Sexually Transmitted Infections, University of Paris, IAME, INSERM 1137, Paris, France

    Francois Camelena & Béatrice Berçot

  6. Bacteriology Department, French National Center for Bacterial Sexually Transmitted Infections, CHU Bordeaux, and USC, University Bordeaux, 3671, Bordeaux, EA, France

    Sabine Pereyre, Marie Gardette & Cécile Bébéar

  7. Department of Dermatology, Hôpital Saint-Louis, Assistance Publique Hôpitaux de Paris, Paris, France

    Jean-David Bouaziz

  8. Team Insight, Université de Paris, INSERM U976, Paris, France

    Jérôme Le Goff & Maud Salmona

  9. Department of Virology, Hôpital Saint-Louis, 1 Avenue Claude Vellefaux, 75010, Paris, France

    Maud Salmona

Authors
  1. Margaux Garzaro
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  2. Lin-Pierre Zhao
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  3. Nathalie De Castro
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  4. Séverine Mercier-Delarue
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  9. Marion Malphettes
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  10. Cécile Bébéar
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  14. Maud Salmona
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Contributions

All authors directly contributed to the patient management. The first draft of the manuscript was written by MG and L-PZ, and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.

Corresponding authors

Correspondence to Margaux Garzaro or Maud Salmona.

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Informed consent of the patient for writing the case was obtained.

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Informed consent of the patient for publication was obtained.

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The authors declare no competing interests.

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Garzaro, M., Zhao, LP., De Castro, N. et al. Metagenomic next-generation sequencing restores the diagnosis of a rare infectious complication of B cell depletion. Eur J Clin Microbiol Infect Dis 41, 1269–1273 (2022). https://doi.org/10.1007/s10096-022-04484-7

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  • DOI: https://doi.org/10.1007/s10096-022-04484-7

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