Skip to main content

Advertisement

SpringerLink
Log in

Human polyomavirus KI, WU, BK, and JC in healthy volunteers

  • Original Article
  • Published:
European Journal of Clinical Microbiology & Infectious Diseases Aims and scope Submit manuscript

Abstract

Despite the growing importance of infections caused by the human polyomaviruses (HPyVs), information about their transmission, pathogenesis, and epidemiology is scarce. The objective of this work was to evaluate the excretion and distribution of HPyV (HPyV1-HPyV4 [former BKPyV, JCPyV, KIPyV, and WUPyV, respectively]) among asymptomatic individuals from different geographic regions in Brazil, in order to verify the existence of distinct epidemiologic patterns among the Brazilian population. Saliva samples from 889 healthy volunteers living in nine locations in Brazil were analyzed by real-time polymerase chain reaction (PCR) to detect HPyV1–4. Among 889 participants, 346 (39%) had evidence of infection with one or more HPyV species: 127 (14.3%) had HPyV1 only; 70 (7.9%) had HPyV3 only; 60 (6.7%) had HPyV4 only, and 25 (2.8%) had HPyV2 only. Coinfections were detected in 64 participants (7.3%). Although HPyV excretion was detected in samples from all locations, the frequency and distribution of viral species varied significantly. The epidemiologic findings presented demonstrate that the four HPyV species studied are circulating in five geographic regions of Brazil. Salivary excretion of these viruses appears common among healthy Brazilians. The distribution of viral species varies considerably between regions as well as within regions.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  1. Moens U, Krumbholz A, Ehlers B, Zell R, Johne R, Calvignac-Spencer S, Lauber C (2017b) Biology, evolution, and medical importance of polyomaviruses: an update. Infect Genet Evol 54:18–38. https://doi.org/10.1016/j.meegid.2017.06.011

    Article  CAS  PubMed  Google Scholar 

  2. Calvignac-Spencer S, Feltkamp M, Daugherty MD, Moens U, Ramqvist T, Johne R, Ehlers B (2015) Revision on the family Polyomaviridae (76 species, four genera). https://talk.ictvonline.org/files/ictv_official_taxonomy_updates_since_the_8th_report/m/animal-dna-viruses-and-retroviruses/5819. Accessed 23 January 2018

  3. Krumbholz A, Bininda-Emonds OR, Wutzler P, Zell R (2009) Phylogenetics, evolution, and medical importance of polyomaviruses. Infect Genet Evol 9(5):784–799. https://doi.org/10.1016/j.meegid.2009.04.008

    Article  CAS  PubMed  Google Scholar 

  4. Barth H, Solis M, Lepiller Q, Sueur C, Soulier E, Caillard S, Stoll-Keller F, Fafi-Kremer S (2017) 45 years after the discovery of human polyomaviruses BK and JC: time to speed up the understanding of associated diseases and treatment approaches. Crit Rev Microbiol 43(2):178–195. https://doi.org/10.1080/1040841X.2016.1189873

    Article  CAS  PubMed  Google Scholar 

  5. Robaina TF, Mendes GS, Benati FJ, Pena GA, Silva RC, Montes MA, Janini ME, Câmara FP, Santos N (2013a) Shedding of polyomavirus in the saliva of immunocompetent individuals. J Med Virol 85(1):144–148. https://doi.org/10.1002/jmv.23453

    Article  Google Scholar 

  6. Robaina TF, Mendes GS, Benati FJ, Pena GA, Silva RC, Montes MA, Otero R, Castro GF, Câmara FP, Santos N (2013b) Polyomavirus in saliva of HIV-infected children, Brazil. Emerg Infect Dis 19(1):155–157. https://doi.org/10.3201/eid1901.120563

    Article  PubMed  PubMed Central  Google Scholar 

  7. Machado DM, Fink MC, Pannuti CS, Succi RC, Machado AA, Carmo FB, Gouvêa AF, Urbano PR, Beltrão SV, Santos IC, Machado CM (2011) Human polyomaviruses JC and BK in the urine of Brazilian children and adolescents vertically infected by HIV. Mem Inst Oswaldo Cruz 106(8):931–935. https://doi.org/10.1590/S0074-02762011000800006

    Article  PubMed  Google Scholar 

  8. Pires EP, Bernardino-Vallinoto CV, Alves DM, Migone SR, Machado LF, Ishak MO, Ishak R, Cayres-Vallinoto IM, Vallinoto AC (2011) Prevalence of infection by JC and BK polyomaviruses in kidney transplant recipients and patients with chronic renal disease. Transpl Infect Dis 13(6):633–637. https://doi.org/10.1111/j.1399-3062.2011.00614.x

    Article  CAS  PubMed  Google Scholar 

  9. Comerlato J, Campos FS, Oliveira MT, Cibulski SP, Corrêa L, Kulmann MIR, Arantes TS, Hentges LP, Spilki FR, Roehe PM, Franco AC (2015) Molecular detection and characterization of BK and JC polyomaviruses in urine samples of renal transplant patients in southern Brazil. J Med Virol 87(3):522–528. https://doi.org/10.1002/jmv.24086

    Article  CAS  PubMed  Google Scholar 

  10. Urbano PR, Oliveira RR, Romano CM, Pannuti CS, Fink MC (2016a) Occurrence, genotypic characterization, and patterns of shedding of human polyomavirus JCPyV and BKPyV in urine samples of healthy individuals in São Paulo, Brazil. J Med Virol 88(1):153–158. https://doi.org/10.1002/jmv.24318

    Article  CAS  PubMed  Google Scholar 

  11. Castro T, Fink MCD, Figueiredo M, Braz-Silva PH, Pannuti CM, Ortega KL, Gallottini M (2017) Polyomavirus BK and JC in individuals with chronic kidney failure, kidney transplantation, and healthy controls. J Clin Virol 89:5–9. https://doi.org/10.1016/j.jcv.2017.02.003

    Article  PubMed  Google Scholar 

  12. Figueiredo MA, Fink MCD, Castro T, Braz-Silva PH, Steffens JC, Eduardo FP, Gallottini M, Ortega KL (2017) Detection of human polyomaviruses JC and BK in liver pretransplant patients. Oral Dis 23(8):1127–1133. https://doi.org/10.1111/odi.12707

    Article  CAS  PubMed  Google Scholar 

  13. Hussain I, Tasneem F, Umer M, Pervaiz A, Raza M, Arshad MI, Shahzad N (2017) Specific and quantitative detection of human polyomaviruses BKPyV and JCPyV in the healthy Pakistani population. Virol J 14(1):86. https://doi.org/10.1186/s12985-017-0752-2

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  14. Egli A, Infanti L, Dumoulin A, Buser A, Samaridis J, Stebler C, Gosert R, Hirsch HH (2009) Prevalence of polyomavirus BK and JC infection and replication in 400 healthy blood donors. J Infect Dis 199(6):837–846

    Article  CAS  PubMed  Google Scholar 

  15. McClure GB, Gardner JS, Williams JT, Copeland CM, Sylvester SK, Garcea RL, Meinerz NM, Groome LJ, Vanchiere JA (2012) Dynamics of pregnancy-associated polyomavirus urinary excretion: a prospective longitudinal study. J Med Virol 84(8):1312–1322. https://doi.org/10.1002/jmv.23320

    Article  PubMed  Google Scholar 

  16. Kling CL, Wright AT, Katz SE, McClure GB, Gardner JS, Williams JT, Meinerz NM, Garcea RL, Vanchiere JA (2012) Dynamics of urinary polyomavirus shedding in healthy adult women. J Med Virol 84:1459–1463. https://doi.org/10.1002/jmv.23319

    Article  PubMed  PubMed Central  Google Scholar 

  17. Holmes EC (2004) The phylogeography of human viruses. Mol Ecol 13(4):745–756. https://doi.org/10.1046/j.1365-294X.2003.02051.x

    Article  PubMed  Google Scholar 

  18. Kitchen A, Miyamoto MM, Mulligan CJ (2008) Utility of DNA viruses for studying human host history: case study of JC virus. Mol Phylogenet Evol 46(2):673–682. https://doi.org/10.1016/j.ympev.2007.09.005

    Article  CAS  PubMed  Google Scholar 

  19. Yogo Y, Sugimoto C, Zhong S, Homma Y (2009) Evolution of the BK polyomavirus: epidemiological, anthropological and clinical implications. Rev Med Virol 19(4):185–199. https://doi.org/10.1002/rmv.613

    Article  CAS  Google Scholar 

  20. Pérez-Losada M, Christensen RG, McClellan DA, Adams BJ, Viscidi RP, Demma JC, Crandall KA (2006) Comparing phylogenetic codivergence between polyomaviruses and their hosts. J Virol 80(12):5663–5669. https://doi.org/10.1128/JVI.00056-06

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  21. Pena SD, Di Pietro G, Fuchshuber-Moraes M, Genro JP, Hutz MH, Kehdy FS, Kohlrausch F, Magno LA, Montenegro RC, Moraes MO, de Moraes ME, de Moraes MR, Ojopi EB, Perini JA, Racciopi C, Ribeiro-Dos-Santos AK, Rios-Santos F, Romano-Silva MA, Sortica VA, Suarez-Kurtz G (2011) The genomic ancestry of individuals from different geographical regions of Brazil is more uniform than expected. PLoS One 6(2):e17063. https://doi.org/10.1371/journal.pone.0017063

    Article  CAS  PubMed  PubMed Central  Google Scholar 

Download references

Acknowledgments

We thank Soluza dos Santos Gonçalves for technical assistance. Soluza dos Santos Gonçalves is the recipient of a fellowship from FAPERJ E-26/202.625/2016. We also thank Dr. Ferando Portela Câmara for the statistical analysis and Maulori Curié Cabral, Tânia Fraga Barros, Raquel Cirlene da Silva, Camila Correia Banks da Rocha, Luz Alba Maria Garcete Fornells Arentz, and Tatiana Ferreira Robaina for assistance with sample collection.

Funding

This study was supported by the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq, grant number, 303864/2014-1), the Fundação Carlos Chagas de Amparo à Pesquisa do Estado do Rio de Janeiro, Brazil (FAPERJ, grant numbers, E-26/201.374/2014 and E-26/202.625/2016), and the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior—Brasil (CAPES)—Finance Code 001. The funders were not involved in study design, data collection, data interpretation, or the decision to submit the work for publication.

Author information

Authors and Affiliations

  1. Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil

    Giselle P. A. Pena, Gabriella S. Mendes, Helver G. Dias, Lucas S. Gavazzoni, Ariane R. Amorim & Norma Santos

  2. Departamento de Virologia, Instituto de Microbiologia, Universidade Federal do Rio de Janeiro, Cidade Universitária, CCS – Bl. I, Ilha do Fundão, Rio de Janeiro, RJ, 21941-902, Brazil

    Norma Santos

Authors
  1. Giselle P. A. Pena
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Gabriella S. Mendes
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Helver G. Dias
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Lucas S. Gavazzoni
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Ariane R. Amorim
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Norma Santos
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Norma Santos.

Ethics declarations

The protocol for this study was reviewed and approved by the Ethics Committee of the Hospital Universitário Clementino Fraga Filho (protocol number 891.574) of the Federal University of Rio de Janeiro, Brazil.

Conflicts of interest

The authors declare that they have no conflicts of interest.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Pena, G.P.A., Mendes, G.S., Dias, H.G. et al. Human polyomavirus KI, WU, BK, and JC in healthy volunteers. Eur J Clin Microbiol Infect Dis 38, 135–139 (2019). https://doi.org/10.1007/s10096-018-3404-6

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10096-018-3404-6

Keywords

Search

Navigation