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Oral shedding of human herpesviruses in renal transplant recipients

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Abstract

Objective

To describe the shedding profile of human herpesviruses in the saliva of renal transplant recipients.

Methods

This is a prospective case-control study of 50 renal transplant recipients and control group of 50 individuals (non-transplanted and immunocompetent). Mouthwash samples were collected via oral rinse and then submitted to screening for the presence of eight types of herpesviruses by using multiplex PCR. Fisher’s exact, chi-square, and Student t tests were used for statistical analysis, and the significance level was set at 5%.

Results

The mean age of the study group was 49.42 ± 12.94 years, 28/50 (56%) were female, and the time elapsed after transplantation was 68.20 ± 67.19 months. Herpes simplex virus 1 (HSV-1) (P = 0.025) and Epstein-Barr virus (EBV) (P = 0.024) were, statistically, more excreted in the saliva of renal transplant recipients compared to control group. Gender (P = 1.00) and age (P = 0.563) did not influence the salivary shedding of herpesviruses in renal transplant recipients. Individuals who excreted varicella-zoster virus in saliva had a shorter mean time of transplantation (22:00 + 2.82 months) (P < 0.001).

Conclusion

Renal transplant recipients excreted herpesviruses more often than controls, especially HSV-1 and EBV, with salivary shedding of herpesviruses being more frequent in patients with recent kidney transplantation.

Clinical relevance

The present findings support other longitudinal studies evaluating the relationship between oral shedding of human herpesviruses and clinical presence of active infection and renal transplant failure.

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Author information

Author notes

  1. Dmitry José de Santana Sarmento and Tânia Regina Tozetto-Mendoza contributed equally to this work.

Authors and Affiliations

  1. Division of Pathology, Department of Stomatology, University of São Paulo School of Dentistry, Av. Prof. Lineu Prestes, 2227 - Cidade Universitária, São Paulo, SP, Brazil

    Dmitry José de Santana Sarmento, Rubens Caliento, Michelle Palmieri, Victor Adriano de Oliveira Martins, Marina Gallottini & Paulo Henrique Braz-Silva

  2. Laboratory of Virology, Institute of Tropical Medicine, University of São Paulo, Av. Dr. Enéas de Carvalho Aguiar, 470 – Jd. América, São Paulo, SP, Brazil

    Tânia Regina Tozetto-Mendoza, Laura Masami Sumita, Claudio Sergio Pannuti & Paulo Henrique Braz-Silva

  3. Department of Infectious Diseases, University of São Paulo School of Medicine, Av. Dr. Arnaldo, 455 - Cerqueira César, São Paulo, SP, Brazil

    Ligia Camara Pierroti

  4. Department of Dentistry, University of Santo Amaro, Rua Professor Enéas de Siqueira Neto, 340 – Santo Amaro, São Paulo, SP, Brazil

    Débora Pallos

Authors
  1. Dmitry José de Santana Sarmento
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  2. Tânia Regina Tozetto-Mendoza
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  3. Laura Masami Sumita
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  4. Ligia Camara Pierroti
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  6. Rubens Caliento
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  7. Michelle Palmieri
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  8. Victor Adriano de Oliveira Martins
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  9. Marina Gallottini
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  10. Claudio Sergio Pannuti
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  11. Paulo Henrique Braz-Silva
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Corresponding author

Correspondence to Paulo Henrique Braz-Silva.

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Conflict of interest

The authors declare that they have no conflict of interest.

Funding

This study was supported by the Government of Brazil through the National Council for Technological Development (CNPq) according to grant number # 443004/2014-5 and São Paulo State Research Support Foundation (FAPESP) according to grant numbers # 2015/03906-6, #2012/04223-1, and #2013/08242-3.

Ethical approval

All the procedures performed in studies involving human participants were in accordance with the ethical standards set by institutional and/or national research committees and with the 1964 Helsinki declaration, including its later amendments or comparable ethical standards. This study was approved by the Research Ethics Committee for Analysis of Research Project (CAPpesq) under protocol number 290/2011.

Informed consent

Written informed consent was individually obtained from all participants included in the study.

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de Santana Sarmento, D.J., Tozetto-Mendoza, T.R., Masami Sumita, L. et al. Oral shedding of human herpesviruses in renal transplant recipients. Clin Oral Invest 22, 885–891 (2018). https://doi.org/10.1007/s00784-017-2166-3

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  • DOI: https://doi.org/10.1007/s00784-017-2166-3

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