Skip to main content

Advertisement

SpringerLink
Log in

Ureaplasma parvum causes hyperammonemia in a pharmacologically immunocompromised murine model

  • Original Article
  • Published:
European Journal of Clinical Microbiology & Infectious Diseases Aims and scope Submit manuscript

Abstract

A relationship between hyperammonemia and Ureaplasma infection has been shown in lung transplant recipients. We have demonstrated that Ureaplasma urealyticum causes hyperammonemia in a novel immunocompromised murine model. Herein, we determined whether Ureaplasma parvum can do the same. Male C3H mice were given mycophenolate mofetil, tacrolimus, and prednisone for 7 days, and then challenged with U. parvum intratracheally (IT) and/or intraperitoneally (IP), while continuing immunosuppression over 6 days. Plasma ammonia concentrations were determined and compared using Wilcoxon rank-sum tests. Plasma ammonia concentrations of immunosuppressed mice challenged IT/IP with spent broth (median, 188 μmol/L; range, 102–340 μmol/L) were similar to those of normal (median, 226 μmol/L; range, 154–284 μmol/L, p > 0.05), uninfected immunosuppressed (median, 231 μmol/L; range, 122–340 μmol/L, p > 0.05), and U. parvum IT/IP challenged immunocompetent (median, 226 μmol/L; range, 130–330 μmol/L, p > 0.05) mice. Immunosuppressed mice challenged with U. parvum IT/IP (median 343 μmol/L; range 136–1,000 μmol/L) or IP (median 307 μmol/L; range 132–692 μmol/L) had higher plasma ammonia concentrations than those challenged IT/IP with spent broth (p < 0.001). U. parvum can cause hyperammonemia in pharmacologically immunocompromised mice.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

References

  1. Chen C, Bain KB, Iuppa JA, Yusen RD, Byers DE, Patterson GA et al (2016) Hyperammonemia syndrome after lung transplantation: a single center experience. Transplantation 100(3):678–684

    Article  CAS  PubMed  Google Scholar 

  2. Uygun V, Karasu G, Daloglu H, Hazar V, Yesilipek A (2015) Idiopathic hyperammonemia after hematopoietic stem cell transplantation: a case report. Pediatr Transplant 19(4):E104–105

  3. Kiberenge RK, Lam H (2015) Fatal hyperammonemia after repeat renal transplantation. J Clin Anesth 27(2):164–167

    Article  PubMed  Google Scholar 

  4. Bharat A, Cunningham SA, Scott Budinger GR, Kreisel D, DeWet CJ, Gelman AE et al (2015) Disseminated Ureaplasma infection as a cause of fatal hyperammonemia in humans. Sci Transl Med 7(284):284re3

    Article  PubMed  PubMed Central  Google Scholar 

  5. Anwar S, Gupta D, Ashraf MA, Khalid SA, Rizvi SM, Miller BW et al (2014) Symptomatic hyperammonemia after lung transplantation: Lessons learnt. Hemodial Int 18(1):185–191

    Article  PubMed  Google Scholar 

  6. Wylam ME, Kennedy CC, Hernandez NM, Peters SG, Maleszewski JJ, Cassivi SD et al (2013) Fatal hyperammonaemia caused by Mycoplasma hominis. Lancet 382(9908):1956

    Article  PubMed  Google Scholar 

  7. Cunningham SA, Mandrekar JN, Rosenblatt JE, Patel R (2013) Rapid PCR detection of Mycoplasma hominis, Ureaplasma urealyticum, and Ureaplasma parvum. Int J Bacteriol 2013:168742

  8. Wang X, Karau MJ, Greenwood-Quaintance KE, Block DR, Mandrekar JN, Cunningham SA et al (2016) Ureaplasma urealyticum causes hyperammonemia in an experimental immunocompromised murine model. PLoS One 11(8):e0161214

    Article  PubMed  PubMed Central  Google Scholar 

  9. Fernandez J, Karau MJ, Cunningham SA, Greenwood-Quaintance KE, Patel R (2016) Antimicrobial susceptibility and clonality of clinical Ureaplasma isolates in the United States. Antimicrob Agents Chemother 60(8):4793–4798

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  10. Waites KB, Katz B, Schelonka RL (2005) Mycoplasmas and Ureaplasmas as neonatal pathogens. Clin Microbiol Rev 18(4):757–789

    Article  PubMed  PubMed Central  Google Scholar 

  11. Gwee A, Curtis N (2014) Ureaplasma—are you sitting comfortably? J Infect 68 [Suppl 1]:S19–23

    Article  PubMed  Google Scholar 

  12. Lichtenstein GR, Yang YX, Nunes FA, Lewis JD, Tuchman M, Tino G et al (2000) Fatal hyperammonemia after orthotopic lung transplantation. Ann Intern Med 132(4):283–287

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgements

We are grateful to Suzannah M. Schmidt Malan and Javier Fernandez Dominguez for their technical input.

Author information

Authors and Affiliations

  1. Division of Clinical Microbiology, Department of Laboratory Medicine and Pathology, Mayo Clinic, 200 First St S.W., Rochester, MN, 55905, USA

    X. Wang, K. E. Greenwood-Quaintance, M. J. Karau, S. A. Cunningham & R. Patel

  2. Center of Infectious Diseases, West China Hospital, Sichuan University, Chengdu, Sichuan, China

    X. Wang

  3. Division of Infectious Diseases, State Key Laboratory of Biotherapy, Chengdu, Sichuan, China

    X. Wang

  4. Division of Clinical Core Laboratory, Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN, USA

    D. R. Block

  5. Department of Health Sciences Research, Mayo Clinic, Rochester, MN, USA

    J. N. Mandrekar

  6. Department of Transplant, Mayo Clinic, Jacksonville, FL, USA

    J. M. Mallea

  7. Division of Infectious Diseases, Department of Medicine, Mayo Clinic, Rochester, MN, USA

    R. Patel

Authors
  1. X. Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. K. E. Greenwood-Quaintance
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. M. J. Karau
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. D. R. Block
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. J. N. Mandrekar
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. S. A. Cunningham
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. J. M. Mallea
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. R. Patel
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to R. Patel.

Ethics declarations

Conflicts of interest

The authors declare that they have no conflicts of interest.

Funding

This work was supported by the Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN, USA.

RP is supported by the National Institutes of Health (grant numbers R01 AR056647 and R01 AI91594). XW was supported by the State Scholarship Fund from the China Scholarship Council as a Visiting Scientist at Mayo Clinic. The funders played no role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript.

Ethical approval

This study was carried out in accordance with recommendations in the Guide for the Care and Use of Laboratory Animals of the National Institutes of Health, and was approved by the Mayo Clinic Institutional Animal Care and Use Committee (protocol number: A8115).

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Wang, X., Greenwood-Quaintance, K.E., Karau, M.J. et al. Ureaplasma parvum causes hyperammonemia in a pharmacologically immunocompromised murine model. Eur J Clin Microbiol Infect Dis 36, 517–522 (2017). https://doi.org/10.1007/s10096-016-2827-1

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10096-016-2827-1

Keywords

Search

Navigation