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The genotypic study of Mycobacterium tuberculosis complex resistant to isoniazid: Galicia, Spain (2008–2013)

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Abstract

Incorporation of rapid detection systems to identify mutations in M. tuberculosis complex that confer resistance to isoniazid and rifampicin has potentiated the knowledge of their distribution, given the geographical variability. We performed antibiograms of the 2,993 strains isolated in Galicia, Spain (2008–2013). In the strains resistant to isoniazid, a concentration of 0.4 mg/mL and MTBDRplus Genotype test (Hain Lifescience, Germany) were used. We found that 3.64 % of strains were resistant to isoniazid, while 0.43 % were resistant to isoniazid and rifampicin (multidrug resistant, MDR). The MTBDRplus test showed an overall sensitivity of 72.48 %, with 62.5 % sensitivity for non MDR isoniazid-resistant strains and 100 % sensitivity for MDR strains. The katG gene mutation was detected at codon 315 in 38.53 % of strains. The S315T mutation appeared in 61.54 % of MDR strains and 34.38 % of non-MDR strains. The 28.44 % had mutations in inhA, (93.55 % in C15T), and 38.46 % of MDR strains were mutated. In non-MDR strains, 37.50 % were wild-type, 35.42 % and 27.08 % had mutations in katG and inhA, respectively. The most frequent mutation in rpoβ was S531L (46.15 %). The 38.71 % and 41.9 % of strains with resistance to isoniazid and streptomycin had mutations in katG and inhA, respectively (2 strains with mutations in T8C and T8A). The distribution pattern of resistance among strains with high and low concentrations of isoniazid showed statistically significant differences in relation to the mutation in katG and wild-type. The sensitivity of the Genotype MTBDRplus test for non-MDR strains in our area was at the lower threshold described.

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Authors and Affiliations

  1. Microbiology Department, Complexo Hospitalario Universitario de Santiago de Compostela, Santiago de Compostela, A Coruña, Spain

    M. L. Pérez del Molino

  2. Microbiology Department, Complexo Hospitalario Universitario de Santiago de Compostela-Universidade de Santiago de Compostela, Santiago de Compostela, A Coruña, Spain

    G. Barbeito-Castiñeiras

  3. Pharmacology Department, Universidade de Santiago de Compostela, Santiago de Compostela, A Coruña, Spain

    B. Mejuto

  4. Microbiology Department, Complexo Hospitalario Universitario de Lugo, Lugo, Spain

    P. Alonso

  5. Microbiology Department, Complexo Hospitalario Universitario de A Coruña, A Coruña, Spain

    A. Fernández

  6. Microbiology Department, Complexo Hospitalario Universitario de Vigo, Vigo, Pontevedra, Spain

    G. González-Mediero

Authors
  1. M. L. Pérez del Molino
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  2. G. Barbeito-Castiñeiras
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  3. B. Mejuto
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  4. P. Alonso
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  5. A. Fernández
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  6. G. González-Mediero
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Consortia

Galician M. tuberculosis Resistance Group

  • Pedro Alonso
  • , Pilar Alonso
  • , Ana Fernàndez
  • , Maria de los Ángeles Gómez-Rial
  • , Gema González-Mediero
  • , Susana Méndez-Lage
  • , Maria Ángeles Pallarés-González
  • , Elizabeth Prieto
  • , Patricia Romero Jung
  •  & María Luisa Pérez del Molino

Corresponding author

Correspondence to G. Barbeito-Castiñeiras.

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Funding

Economic funding was not received.

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

Isolates were obtained as part of the routine activity of the GMRL and were analysed anonymously in a retrospective manner. Ethical approval and informed consent were, thus, not required.

Appendix: Galician M. tuberculosis Resistance Group

Appendix: Galician M. tuberculosis Resistance Group

Microbiology Department, Hospital Comarcal de Monforte de Lemos:

Pedro Alonso

Microbiology Department, Complexo Hospitalario de Lugo:

Pilar Alonso

Microbiology Department, Complexo Hospitalario Universitario de A Coruña:

Ana Fernández

Microbiology Department, Hospital Comarcal de O Barco de Valdeorras:

María de los Ángeles Gómez-Rial

Microbiology Department, Complexo Hospitalario Universitario de Vigo:

Gema González-Mediero

Microbiology Department, Complexo Hospitalario Universitario de Ferrol:

Susana Méndez-Lage

Microbiology Department, Complexo Hospitalario Universitario de Pontevedra:

María Ángeles Pallarés-González

Microbiology Department, Hospital da Costa Burela:

Elizabeth Prieto

Microbiology Department, Complexo Hospitalario Universitario de Ourense:

Patricia Romero Jung

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Pérez del Molino, M.L., Barbeito-Castiñeiras, G., Mejuto, B. et al. The genotypic study of Mycobacterium tuberculosis complex resistant to isoniazid: Galicia, Spain (2008–2013). Eur J Clin Microbiol Infect Dis 35, 1795–1801 (2016). https://doi.org/10.1007/s10096-016-2730-9

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  • DOI: https://doi.org/10.1007/s10096-016-2730-9

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