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Evaluation of matrix-assisted laser desorption/ionisation time-of-flight (MALDI-TOF) mass spectrometry for identification of Candida parapsilosis, C. orthopsilosis and C. metapsilosis

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Abstract

We have evaluated matrix-assisted laser desorption/ionisation time-of-flight (MALDI-TOF) mass spectrometry for the rapid identification of Candida parapsilosis, C. orthopsilosis and C. metapsilosis. A total of 103 isolates, including reference strains and clinical isolates, were identified by pyrosequencing of the ITS1 region and then assay by MALDI-TOF mass spectrometry. Concordance between the two methods was 100%, showing that MALDI-TOF may be useful as a rapid and reliable method for discrimination of species within the C. parapsilosis group.

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References

  1. Levin AS, Costa SF, Mussi NS, Basso SI, Sinto SI, Machado SC, Geiger DC, Villares MCB, Schreiber AZ, Barone AA, Branchini MLM (1998) Candida parapsilosis fungaemia associated with implantable and semi-implantable central venous catheters and the hands of healthcare workers. Diagn Microbiol Infect Dis 30:243–249

    Article  PubMed  CAS  Google Scholar 

  2. Lupetti A, Tavanti A, Davini O, Ghelardi E, Corsini I, Merusi A, Boldrini A, Campa M, Senesi S (2002) Horizontal transmission of Candida parapsilosis candidaemia in a neonatal intensive care unit. J Clin Microbiol 40:2363–2369

    Article  PubMed  Google Scholar 

  3. Kojic EM, Darouiche RO (2004) Candida infections of medical devices. Clin Microbiol Rev 17:255–267

    Article  PubMed  Google Scholar 

  4. Weems JJ Jr (1992) Candida parapsilosis: epidemiology, pathogenicity, clinical manifestations, and antimicrobial susceptibility. Clin Infect Dis 14:756–766

    Article  PubMed  Google Scholar 

  5. Lehmann PF, Lin DM, Lasker BA (1992) Genotypic identification and characterization of species and strains within the genus Candida by using random amplified polymorphic DNA. J Clin Microbiol 30:3249–3254

    PubMed  CAS  Google Scholar 

  6. Lin D, Wu LC, Rinaldi MG, Lehmann PF (1995) Three distinct genotypes within Candida parapsilosis from clinical sources. J Clin Microbiol 33:1815–1821

    PubMed  CAS  Google Scholar 

  7. Kato M, Ozeki M, Kikuchi A, Kanbe T (2001) Phylogenetic relationship and mode of evolution of yeast DNA topoisomerase II gene in the pathogenic Candida species. Gene 272:275–281

    Article  PubMed  CAS  Google Scholar 

  8. Nosek J, Tomaska L, Rycovska A, Fukuhara H (2002) Mitochondrial telomeres as molecular markers for identification of the opportunistic yeast pathogen Candida parapsilosis. J Clin Microbiol 40:1283–1289

    Article  PubMed  CAS  Google Scholar 

  9. Tavanti A, Davidson AD, Gow NA, Maiden MC, Odds FC (2005) Candida orthopsilosis and Candida metapsilosis spp. nov. to replace Candida parapsilosis groups II and III. J Clin Microbiol 43:284–292

    Article  PubMed  CAS  Google Scholar 

  10. Borman AM, Linton CJ, Oliver D, Palmer MD, Szekely A, Odds FC, Johnson M (2009) Pyrosequencing analysis of 20 nucleotides of internal transcribed spacer 2 discriminates Candida parapsilosis, Candida metapsilosis and Candida orthopsilosis. J Clin Microbiol 47:2307–2310

    Article  PubMed  CAS  Google Scholar 

  11. Lockhart SR, Messer SA, Pfaller MA, Diekema DJ (2008) Geographic distribution and antifungal susceptibility of the newly described species Candida orthopsilosis and Candida metapsilosis in comparison to the closely related species Candida parapsilosis. J Clin Microbiol 46:2659–2664

    Article  PubMed  CAS  Google Scholar 

  12. Gómez-López A, Alatruey-Izquierdo A, Rodríguez D, Almirante B, Pahissa A, Rodríguez-Tudela JL, Cuenca-Estrella M, the Barcelona Candidemia Project Study Group (2008) Prevalence and susceptibility profile of Candida metapsilosis and Candida orthopsilosis: results from population-based surveillance of Candidemia in Spain. Antimicrob Agents Chemother 52:1506–1509

    Article  PubMed  Google Scholar 

  13. Degand N, Carbonnelle E, Dauphin B, Beretti J, Le Bourgeois LM, Semet-Gaudelus C, Segonds BP, Nassif X, Ferroni A (2008) Matrix-assisted laser desorption ionization-time of flight mass spectrometry for identification of nonfermenting gram-negative bacilli isolated from cystic fibrosis patients. J Clin Microbiol 46:3361–3367

    Article  PubMed  CAS  Google Scholar 

  14. Marklein G, Josten M, Klanke U, Müller E, Horré R, Maier T, Wenzel T, Kostrzewa M, Bierbaum G, Hoerauf A, Sahl HG (2009) Matrix-assisted laser desorption ionization-time of flight mass spectrometry for fast and reliable identification of clinical yeast isolates. J Clin Microbiol 47:2912–2917

    Article  PubMed  CAS  Google Scholar 

  15. Marinach-Patrice C, Lethuillier A, Marly A, Brossas JY, Gene J, Symoens F (2009) Use of mass spectrometry to identify clinical Fusarium isolates. Clin Microbiol Infect 15:634–642

    Article  PubMed  CAS  Google Scholar 

  16. Seng P, Drancourt M, Gouriet F, La Scola B, Fournier PE, Rolain JM, Raoult D (2009) Ongoing revolution in bacteriology: routine identification of bacteria by matrix-assisted laser desorption ionization time-of-flight mass spectrometry. Clin Infect Dis 49:543–551

    Article  PubMed  CAS  Google Scholar 

  17. Stevenson LG, Drake SK, Shea YR, Zelazny AM, Murray PR (2010) Evaluation of matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF) for identification of clinically important yeast species. J Clin Microbiol 48:3482–3486

    Article  PubMed  CAS  Google Scholar 

  18. Cantón E, Espinel-Ingroff A, Pemán J, del Castillo L (2010) In vitro fungicidal activities of echinocandins against Candida metapsilosis, C. orthopsilosis, and C. parapsilosis evaluated by time-kill studies. Antimicrob Agents Chemother 54:2194–2197

    Article  PubMed  Google Scholar 

  19. De Toro M, Torres MJ, Maite R, Aznar J (2011) Characterization of Candida parapsilosis complex isolates. Clin Microbiol Infect 17:418–424

    Article  PubMed  Google Scholar 

  20. Melo AS, Bizerra F, Freymüller E, Arthington-Skaggs BA, Colombo AL (2011) Biofilm production and evaluation of antifungal susceptibility amongst clinical Candida spp. isolates, including strains of the Candida parapsilosis complex. Med Mycol 49:253–262

    Article  PubMed  CAS  Google Scholar 

  21. Orsi CF, Colombari B, Blasi E (2010) Candida metapsilosis as the least virulent member of the 'C. parapsilosis'’ complex. Med Mycol 48:1024–1033

    Article  PubMed  Google Scholar 

  22. Bader O, Weig M, Taverne-Ghadwal L, Lugert R, Groß U, Kuhns M (2010) Improved clinical laboratory identification of human pathogenic yeasts by matrix-assisted laser desorption ionization time-of-flight mass spectrometry. Clin Microbiol Infect doi: 10.1111/j.1469-0691.2010.03398.x

    PubMed  Google Scholar 

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Acknowledgements

J.M. was recipient of a Ramón y Cajal fellowship financed by the European Social Fund and the Ministerio de Ciencia e Innovación.

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Authors and Affiliations

  1. Servicio de Microbiología, Hospital Universitario La Paz, IdiPAZ, Paseo de La Castellana 261, 28046, Madrid, Spain

    I. Quiles-Melero, J. García-Rodríguez & J. Mingorance

  2. Servicio de Micología, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Ctra Majadahonda-Pozuelo Km 2, Majadahonda, 28220, Madrid, Spain

    A. Gómez-López

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  1. I. Quiles-Melero
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  2. J. García-Rodríguez
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  3. A. Gómez-López
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  4. J. Mingorance
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Correspondence to J. Mingorance.

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Quiles-Melero, I., García-Rodríguez, J., Gómez-López, A. et al. Evaluation of matrix-assisted laser desorption/ionisation time-of-flight (MALDI-TOF) mass spectrometry for identification of Candida parapsilosis, C. orthopsilosis and C. metapsilosis . Eur J Clin Microbiol Infect Dis 31, 67–71 (2012). https://doi.org/10.1007/s10096-011-1277-z

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  • DOI: https://doi.org/10.1007/s10096-011-1277-z

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