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Evaluation of the Immunoquick+4 malaria rapid diagnostic test in a non-endemic setting

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Abstract

The aim of this retrospective study was to evaluate the Immunoquick+4 (BioSynex, Strasbourg, France), a three-band malaria rapid diagnostic test (MRDT) targeting histidine-rich protein-2 (HRP-2) and pan Plasmodium-specific parasite lactate dehydrogenase, in a non-endemic reference setting. Stored whole-blood samples (n = 613) from international travellers suspected of malaria were used, with microscopy corrected by polymerase chain reaction (PCR) as the reference method. Samples infected by P. falciparum (n = 323), P. vivax (n = 97), P. ovale (n = 73) and P. malariae (n = 25) were selected, as well as 95 malaria-negative samples. The overall sensitivities of the Immunoquick+4 for the diagnosis of P. falciparum, P. vivax, P. malariae and P. ovale were 88.9, 75.3, 56.0 and 19.2%, respectively. Sensitivity was significantly related to parasite density for P. falciparum (93.6% versus 71.4% at parasite densities >100/µl and ≤100/µl, respectively) and P. vivax (86.8% versus 48.3% at parasite densities >500/µl and ≤500/µl, respectively). The Immunoquick+4 showed good reproducibility and reliability for both test results and line intensities. The Immunoquick+4 performed well for the detection of P. falciparum and P. vivax.

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Acknowledgements

We would like to thank the staff of the Central Laboratory of Clinical Biology for their technical support.

Authors’ contributions

DvD, PG and JJ designed the study protocol. MvE and EV organised prospective sample collection. DvD and PG carried out the test evaluations, LC performed PCR analysis. DvD, PG and JJ analysed and interpreted the results and drafted the manuscript. DvD performed the statistical analysis. All authors contributed to the discussion of the results and the redaction of the manuscript; they all approved the final manuscript.

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Authors and Affiliations

  1. Medical Microbiology, Faculty of Health, Medicine and Life Sciences (FHML), Maastricht University, Maastricht, The Netherlands

    D. P. J. van Dijk & J. Jacobs

  2. Department of Clinical Sciences, Unit of Tropical Laboratory Medicine, Institute of Tropical Medicine (ITM), Nationalestraat 155, 2000, Antwerp, Belgium

    P. Gillet, E. Vlieghe, L. Cnops, M. Van Esbroeck & J. Jacobs

Authors
  1. D. P. J. van Dijk
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  2. P. Gillet
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  3. E. Vlieghe
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  4. L. Cnops
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  5. M. Van Esbroeck
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  6. J. Jacobs
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Corresponding author

Correspondence to P. Gillet.

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van Dijk, D.P.J., Gillet, P., Vlieghe, E. et al. Evaluation of the Immunoquick+4 malaria rapid diagnostic test in a non-endemic setting. Eur J Clin Microbiol Infect Dis 29, 577–583 (2010). https://doi.org/10.1007/s10096-010-0898-y

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  • DOI: https://doi.org/10.1007/s10096-010-0898-y

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