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Role of lipase in Burkholderia cepacia complex (Bcc) invasion of lung epithelial cells

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Abstract

The Burkholderia cepacia complex (Bcc) is a group of ten closely related species associated with life-threatening infection in cystic fibrosis (CF). These bacteria are highly antibiotic resistant, with some strains transmissible, and in a subgroup of patients, they can cause a rapid and fatal necrotising pneumonia. The Bcc organisms produce a range of exoproducts with virulence potential, including exopolysaccharide, proteases and lipases. Many members of the Bcc are also capable of epithelial cell invasion, although the mechanism(s) involved are poorly understood. This study investigates a role for Bcc lipase in epithelial cell invasion by Bcc strains. Lipase activity was measured in eight species of the Bcc. Strains that produced high levels of lipase were predominantly from the B. multivorans and B. cenocepacia species. Pre-treatment of two epithelial cell lines with Bcc lipase significantly increased invasion by two B. multivorans strains and one B. cenocepacia strain and did not affect either plasma membrane or tight junction integrity. Inhibition of Bcc lipase production by the lipase inhibitor Orlistat significantly decreased invasion by both B. multivorans and B. cenocepacia strains in a concentration-dependent manner. This study demonstrates the extent of lipase production across the Bcc and establishes a potential role for lipase in Bcc epithelial cell invasion.

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Acknowledgements

The authors would like to express their gratitude to Dr. Dieter Gruenert, California Pacific Medical Center Research Institute, San Francisco, CA for kindly providing us with the 16HBE14o− and CFBE41o− cell lines. In addition, we would like to thank Dr. Peter Vandamme at the Laboratory for Microbiology, Ghent University, Ghent Belgium for providing the Bcc strains. This work was funded by the Post-graduate R&D Skills Programme (PRDSP) and the Programme for Research in Third Level Institutions (PRTLI) administered by the Higher Education Authority (HEA), Ireland.

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Author notes

  1. K. Markey

    Present address: Division of Bacteriology, National Institute for Biological Standards and Control, Blanche Lane, South Mimms Potters Bar, Hertfordshire, EN6 3QG, UK

Authors and Affiliations

  1. Department of Applied Science and National Institute of Cellular Biotechnology, Institute of Technology, Tallaght – Dublin 24, Ireland

    T. Mullen, K. Markey, S. McClean & M. Callaghan

  2. Adelaide and Meath Hospitals Incorporating the National Children’s Hospital, Tallaght – Dublin 24, Ireland

    P. Murphy

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  1. T. Mullen
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  4. S. McClean
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  5. M. Callaghan
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Correspondence to M. Callaghan.

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Mullen, T., Markey, K., Murphy, P. et al. Role of lipase in Burkholderia cepacia complex (Bcc) invasion of lung epithelial cells. Eur J Clin Microbiol Infect Dis 26, 869–877 (2007). https://doi.org/10.1007/s10096-007-0385-2

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