Abstract
Type VI secretion systems (T6SS), recently described in hypervirulent K. pneumoniae (hvKp) strains, are involved in bacterial warfare but their role in classical clinical strains (cKp) has been little investigated. In silico analysis indicated the presence of T6SS clusters (from zero to four), irrespective of the strains origin or virulence, with a high prevalence in the K. pneumoniae species (98%). In the strain CH1157, two T6SS-apparented pathogenicity islands were detected, T6SS-1 and -2, harboring a phospholipase-encoding gene (tle1) and a potential new effector-encoding gene named tke (Type VI Klebsiella effector). Tle1 expression in Escherichia coli periplasm affected cell membrane permeability. T6SS-1 isogenic mutants colonized the highest gastrointestinal tract of mice less efficiently than their parental strain, at long term. Comparative analysis of faecal 16S sequences indicated that T6SS-1 impaired the microbiota richness and its resilience capacity. Oscillospiraceae family members could be specific competitors for the long-term gut establishment of K. pneumoniae.
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Introduction
Klebsiella pneumoniae is an opportunistic Gram-negative pathogen that is considered by the World Health Organization (WHO) and the Center for Disease Control and Prevention (CDC) to be a major health threat. Classical clinical isolates are responsible for hospital-acquired infections such as urinary tract infections, pneumonia, meningitis and endophthalmitis. K. pneumoniae is responsible for severe disseminated infections after broad-spectrum antibiotic treatment1, partly due to its intrinsic resistance to β-lactam antibiotics combined with recently acquired resistance to carbapenems2,3. Hypervirulent K. pneumoniae (hvKp) are also responsible for severe community-acquired infections and are particularly associated with pyogenic liver abscesses4,5,6. Their enhanced virulence is due to the overproduction of factors such as siderophores and capsular polysaccharides. The hypervirulence phenotype is linked to the presence of a plasmid (pLVPK) that harbors several genes or operons (rmpA, magA and iro and iuc operons) involved in the synthesis of virulence factors7,8,9,10. K. pneumoniae is able to survive in different environments such as soil, plants, dust, and water11,12, and human infections are usually preceded by gastro-intestinal colonization1. The mechanisms allowing K. pneumoniae to establish within the intestinal microbiota are poorly understood but they are likely to require a competitive advantage over resident intestinal microbiota13.
In Gram-negative pathogens, secretion systems are involved in bacterial fitness14,15,16. The Type VI Secretion System (T6SS) was initially described in Vibrio cholerae as an extracellular protein translocation system17 and has since been largely documented in Bacteroidetes and Proteobacteria, especially in Enterobacteriaceae18. It consists of a molecular syringe functionally and structurally related to the contractile tail of bacteriophages19. T6SS apparatus is composed of three major subunits: a periplasmic anchored baseplate complex, an inner syringe made up of Hcp protein hexamers fixed to a VgrG/PAAR protein tip complex acting like a puncture device. Once fully assembled, the TssBC contractile tube sheath engulfs the inner syringe leading to the T6SS effector extrusion. After contraction, an ATPase, ClpV, is engaged to recycle the different subunits allowing further attacks20. Bacteria harboring T6SS are thus able to inject toxic effectors in adjacent cells via direct contact leading to the death of preys21. Different kinds of effectors have been described according to their mode of action including DNAse, phospholipase, glycoside, and hydrolase22. Each toxic effector has an associated cognate immunity protein that prevents T6SS-carrying bacteria from self-intoxication and acts as a paired toxin-antitoxin system22,23. The presence of T6SS confers an advantage in highly competitive environments such as host gut microbiota24,25. For instance, the intestinal pathogen Salmonella Typhimurium T6SS provides antibacterial activity against specific gut microbiota individuals such as K. oxytoca and K. variicola, suggesting that it kills microbiota members to invade the mice gut26. Shigella sonei is also able to use its T6SS to eliminate gut competitors in vivo (E. coli and Shigella flexneri) in mice27.
Sarris et al. provided first evidence of T6SS in K. pneumoniae species in 201128. The first K. pneumoniae T6SS effector, a phospholipase (Pld1), was then identified and its role in pulmonary infection was demonstrated in a murine model using a pld1 deletion mutant strain29. More recently, analysis of 107 fully sequenced genomes of K. pneumoniae identified 254 T6SS related clusters30, and their presence was positively correlated with the hypervirulent phenotype31,32. T6SS are conserved worldwide across the genome of K. pneumoniae sequence type 258 (ST258) causing health-care-associated pneumonia33. In vitro intra- and inter-species antibacterial activities have been associated with T6SS expression, and a phospholipase (Tle1KP) was identified as the effector of the multidrug resistant strain HS11286 and a toxin-antitoxin system including a phospholipase effector (Tle1KP) and its cognate immunity protein (Tli1KP) was identified in the multidrug resistant strain HS11286, conferring Tle1KP resistance30. Other phospholipase T6SS effectors have already been identified in K. pneumoniae species (Tle3)34, but due to the diversity of T6SS effectors35,36 others remain to be identified.
Most data so far have been obtained with hvKp isolates, and the prevalence and function of T6SS in classical K. pneumoniae (cKp) strains have not been fully investigated despite the strains being capable of colonizing the intestinal tract. In this study, an in silico approach was used to investigate the prevalence of T6SS and its effectors among documented Klebsiella database. The cKp CH1157 strain was used to investigate the toxic effect of Tle1 in vitro using site-directed Escherichia coli toxicity assays. In addition, intestinal colonization assays were performed in a murine model to assess the role of T6SS-1 in colonization kinetics of the intestinal tract and its impact on intestinal microbiota composition via 16S rRNA high-throughput sequencing.
Results
Prevalence of T6SS clusters among Klebsiella genus and genome organization of T6SS islands in CH1157 strain
The prevalence of T6SS in Klebsiella genomes was assessed using MacSyFinder37 and custom-made T6SS models38 within 423 complete genomes of the Klebsiella genus of which 339 belonged to K. pneumoniae and 22 to K. variicola (Supplementary Table S1a). Both K. pneumoniae and K. variicola had a high prevalence of T6SS clusters, with 98% (332 out of 339) and 95% (20 out of 21) of the strains harboring at least one T6SS, respectively (Fig. 1a). T6SS was present in a remarkably low proportion of K. quasi subsp similipneumoniae representatives (2 out of 14). To further investigate this distribution, we downloaded 45 other complete genomes of K. quasi subsp similpneumoniae (Supplementary Table S1b). Their inclusion did not significantly alter the results, with a global prevalence of 0.2 (in 12 out of the 59 analysed). On average, 1.99 T6SS clusters per genome were found, ranging from 0 to 4 across genomes considering the presence of at least 8 different clustered proteins among TssA, TssB, TssC, TssD (Hcp), TssE, TssF, TssG, TssH (ClpV), TssI (VgrG), TssJ, TssK, TssL, TssM and EvpJ (PAAR) (Fig. 1b, Supplementary Fig. S1). Decreasing the number of proteins required for T6SS systems to be considered complete increased the number of T6SS clusters in genomes that already had a complete T6SS (Supplementary Fig. S2b and c). Accordingly, genomes without a full system (by considering 8 core proteins) did not harbor incomplete T6SS operons (Supplementary Fig. S2b and c).
No significant association was found between the presence of rmpA (a capsule regulator involved in hypermucoid phenotype and a proxy of hypervirulence) and the number of T6SS clusters in a genome (Kruskal–Wallis Test, P = 0.7) (Supplementary Table S1a). Similarly, no association was observed between the number of T6SS clusters and the virulence score of Klebsiella (Spearman’s test, P = 0.7) (Supplementary Table S1a). Using a smaller database (KL65) representative of the diversity of Klebsiella and curated for strain origin (see methods) evidenced no significant association between the origin of the strain and the prevalence of T6SS (Fisher’s Exact Test, P = 0.13) (Fig. 1b).
In the laboratory model strain CH1157, two T6SS-apparented pathogenicity islands were detected, hereafter referred to as T6SS-1 and -2 (27 and 21 kb, respectively). T6SS-1 is closely related to T6SS-1 of reference hypervirulent NTUH-K2044 strain owing their genomic organization and the synteny between the two T6SS core gene loci (Fig. 1c). In contrast, the regions between vgrG and tssM (corresponding to the toxin-antitoxin system) are highly specific to each strain and likely possess specific T6SS dependent effector encoding genes. T6SS-1 of strain CH1157 encompasses a potentially phospholipase encoding gene (tle1) comprising a DUF2235 domain and its cognate three antitoxin genes named tli1 whereas NTUH-K2044 possesses a LysM gene with a peptidoglycan binding domain and an MltD gene (murein lytic transglycosylase). In the KL65 database, the presence of Tle1 phospholipase toxic effector seems to be specific to the K. pneumoniae species complex, since it was detected only in 18 K. pneumoniae strains, 2 K. variicola strains (342 and At-22) and 1 K. quasivariicola (Fig. 1b).
The CH1157 T6SS-2 cluster differs slightly from T6SS-1, having fewer core genes (tssBC, hcp, PAAR, clpV missing) and a different cluster organization. However, synteny was observed between T6SS-2 locus and the second T6SS cluster of K. variicola 342 (Fig. 1c). The effector in CH1157 T6SS-2 was different from that of T6SS-1 and potentially encodes an endopeptidase M23-like protein. This hypothetical protein comprises an N-Terminal LysM peptidoglycan binding domain (PF 01476.23), a SLT transglycosylase domain (PF 01464.23), and a C-Terminal M23 peptidase family (PF 01551.25). The prevalence of such a M23-like protein was investigated in silico in 13514 complete genomes from 4232 different bacterial species (Supplementary Fig. S3). The presence of this protein seems to be exclusive to Klebsiella genus and K. pneumoniae species and was thus named “Tke” (Type VI Klebsiella Effector) following the nomenclature of T6SS bacterial effectors (Fig. 1b). No cognate immunity protein was identified.
Membrane permeability associated with periplasmic expression of Tle1 phospholipase
The Tle1 effector antibacterial effect has been previously reported30. To assess if this phenotype was associated with the effector of strain CH1157, heterologous expression of Tle1 in E. coli DH5α was performed. Addressing of Tle1 specifically to the periplasm significantly affected bacterial growth, as evidenced by spectrometric measurement of the growth of E. coli DH5α/pBAD33-ssOmpA-tle1 compared to that of the control strain (E. coli DH5α/pBAD33-ssOmpA) (Fig. 2a). In contrast, cytoplasmic addressing of Tle1 (E. coli DH5α recombinant strain harboring pBAD33-RBS-tle1) did not affect bacterial growth compared to that of the control strain (E. coli DH5α/pBAD33-RBS). Determination of the number of viable cells at the final point (8 h) did not show any significant differences between the four strains (Fig. 2b) but an unusual microcolony phenotype was associated with periplasmic addressing of Tle1 (Fig. 2c). Flow cytometry analysis after SYBR Green and propidium iodide (IP) staining showed that when Tle1 was located in the periplasm it significantly enhanced cell membrane permeability compared to Tle1 in the cytoplasm and DH5α control strains (DH5α/pBAD33-ssOmpA and DH5α/pBAD33-RBS) (Fig. 2d).
K. pneumoniae T6SS-1 is involved in mice intestinal colonization
In absence of streptomycin treatment, the colonization of the gut by K. pneumoniae CH1476 (a fully virulent CH1157-derived strain39 (see Material and Methods)) could not be followed in feces due to its very low implantation until 10 days post- infection (5.21.102 ± 2.17. 102 CFU/g of feces; detection threshold: 102 CFU/g of feces). In the streptomycin-treated murine model, whatever the strain tested (CH1476 wild-type harboring the empty vector, the isogenic mutant CH1476-ΔtssB/pSTAB or the trans-complemented strain CH1476-ΔtssB/pSTAB-tssB) (Fig. 3a), a peak of colonization level was reached after 7 days of infection (109 UFC/g of feces), followed by stable colonization kinetics until 54 days post-infection (Fig. 3b).
CH1476-ΔtssB/pSTAB mutant strain then became undetectable in the stool 61 days post-infection while the CH1476-ΔtssB/pSTAB-tssB trans-complemented and CH1476/pSTAB WT strains remained detectable until 96 and 145 days post-infection, respectively. Analysis of the intestinal content of the three sets of mice on day 145 post-infection showed that CH1476-ΔtssB/pSTAB was mainly present in the lower part of the gastrointestinal tract, whereas the CH1476/pSTAB WT and the trans-complemented strains were detected at similar levels in the proximal intestine and the colon (between 103 and 104 CFU/g of content) (Fig. 3c). Similar results were obtained with the CH1476-ΔclpV mutant, which is deficient in T6SS recycling. CH1476-ΔclpV strain colonization levels were significantly lower than those of the CH1476 parental strain 80 days after infection (3.1.104 CFU/g of feces vs 8.5.106 CFU/g of feces respectively). In addition, analysis of intestinal contents showed that colonization levels of CH1476-ΔclpV were significantly lower all along the intestinal tract than those of the CH1476 parental strain (Supplementary Fig. S4).
Influence of T6SS-1 on the richness and diversity of intestinal microbiota in a streptomycin-treated mice model
Overall microbiota diversity was greatly affected by initial streptomycin treatment in every animal group, as shown by the decrease in α-diversity indexes (Observed and Shannon) between D-3 and D0 (Fig. 4a and b). Principal coordinate analysis (PCoA) of unweighted UniFrac non-metric multidimensional scaling (NMDS) (β-diversity) showed that the microbial composition of mice microbiota was clearly impacted due to the streptomycin treatment (D-3 versus D0) (Fig. 4c). Some bacterial families were not affected by streptomycin administration (Porphyromonadaceae, Bifidobacteriaceae, Atopobiaceae, Rikenellaceae) while some others were significantly depleted (Bacteroidales, Prevotellaceae, Coriobacteriaceae, Oscillospiraceae, Defluvitaleaceae, Clostridiaceae, Anaerovoraceae, Rhodospirillaceae, Desulfovibrionaceae, Deferribacteraceae, Alcaligenaceae) (Fig. 4d; Supplementary Fig. S6b).
In animals receiving the K. pneumoniae wild-type strain (CH1476/pSTAB), the diversity of the microbiota remained low even after 50 days, whereas in animals receiving the mutant strain (CH1476-∆tssB/pSTAB) diversity increased (Fig. 4a and b). Diversity was only partially restored in mice receiving the trans-complemented strain (CH1476-∆tssB/pSTAB-tssB) (Fig. 4a and b). At D + 50, the β-diversity of each group of mice (receiving CH1476/pSTAB, CH1476-ΔtssB/pSTAB and CH1476-ΔtssB/pSTAB-tssB) was reflected in different clusters (Fig. 4c), indicating a significant effect of the cKp T6SS-1, which is likely the system responsible for delivering Tle1, on the resilience of the intestinal microbiota. Similar results were obtained with the CH1476-ΔclpV mutant compared to the CH1476 WT strain in in vivo assays (Supplementary Fig. S5).
Further analysis of the composition of the microbiota members in each group of mice by 16S sequencing of animal fecal content indicated that Clostridiales, Enterobacteriaceae and Rhodospirillaceae were present at both D-3 and D+50 in mice infected with the CH1476/pSTAB WT strain (Fig. 5a,c, and d). In the group of mice infected by CH1476-ΔtssB/pSTAB-tssB, Alcaligenaceae, Clostridiales and Anaerovoraceae families were found at both D-3 and D+50 (Fig. 5c and d; Supplementary Fig. S6a). In the group of mice infected by the CH1476-∆tssB/pSTAB strain, Oscillospiraceae and Alcaligenaceae families were found at D-3 and D+50 (Fig. 5b, c and d). In mice infected with the CH1476-ΔclpV mutant, Oscillospiraceae were also found at D+50 (Supplementary Fig. S7). These results suggest that Oscillospiraceae could be a potential target of T6SS of K. pneumoniae. Additionally, enriched and depleted families in each group were analyzed at D+50 post-infection. A 6 and 12.1-fold increase was found at D+50 in the Oscillospiraceae family in mice groups receiving, respectively, CH1476-ΔclpV or CH1476-ΔtssB/pSTAB mutant in comparison to their respective control WT group (Fig. 5e; Supplementary Fig. S7f.). Clostridiaceae was also over-represented in the two groups of mice receiving the mutant strains at D+50 in comparison with their respective WT groups (13 and 2.47-fold increase, respectively).
Discussion
In agreement with previously published data28,29,33,36,40, in silico analysis detected T6SS-related genes in both hypervirulent and non-hypervirulent K. pneumoniae strains and in other Klebsiella species irrespective of the strains’ virulence and their origin of isolation. The T6SS prevalence ranged from 98% in K. pneumoniae species to 14% in K. quasipneumoniae similpneumoniae, suggesting that a strong counter selection had occurred in this subspecies, which includes mostly lower virulent strains with less extensive antibiotic resistance41. Two T6SS-apparented clusters, T6SS-1 and T6SS-2, were identified in the genome of the classical K. pneumoniae CH1157 strain. The cluster organization of T6SS-1 presents a synteny with that of T6SS-1 of the hypervirulent strain NTUH-K204442. A toxin-antitoxin system was also identified in the T6SS-1 cluster composed of a type VI lipase effector 1 encoding gene (tle1). In accordance with the classification proposed by Li et al., a DUF2235 domain is present in the protein sequence encoded by the tle1 gene, its specific function is not known. The presence of such a domain in CH1157 Tle1, allows its affiliation into the 4th effector group36. Although 80% of ST11 isolates of K. pneumoniae belong to this group, this is not the case for the ST2 isolate CH1157 strain. As previously mentioned, three tli1 encoding genes, which could serve as Tle1 immunity proteins, were found upstream tle1 in T6SS-1 cluster36. Tle1 phospholipase recruitment requires a DUF4123 adaptor to be fixed on the tip of the T6SS syringe before delivery. This effector chaperone is located upstream of the toxin-antitoxin system and has been found in other T6SS operons43,44. The presence of this potential lipase effector Tle1 was detected in 57% of K. pneumoniae isolates, a proportion consistent with that of previous published data obtained with Gram-negative species (Burkholderia thailandensis, E. coli, Pseudomonas aeruginosa, S. enterica, Acinetobacter baumanii)34. The CH1157 tle1 gene has a 92% nucleotide identity coverage compared with the tle1 sequence previously described in the multidrug-resistant K. pneumoniae strain HS1128630.
The role of T6SS in K. pneumoniae species has been so far studied mainly in hypervirulent strains30,31,32,42. We aimed to identify the potential antibacterial effect of K. pneumoniae CH1157 Tle1. As reported in another non-hypervirulent but multi-drug resistant strain, HS11286, the Tle1 effector of K. pneumoniae CH1157 strain could exert an antibacterial effect30. This toxic effect was first assessed by in vitro competition assays in i) an interspecies manner, by co-cultivating a CH1157 attacker strain and a variety of Gram-negative potential prey strains representative of those found in mice microbiota (E. coli, Bacteroides thetaiotaomicron, Bacteroides fragilis, Parabacteroides dystasonis) and ii) in an intraspecies manner, by co-cultivating a CH1157 attacker strain and its T6SS-derived mutant strains impaired either for T6SS core gene synthesis (CH1476-ΔtssB) or for toxin-antitoxin system production (CH1476-ΔTA), respectively (data not shown). No antibacterial effect was observed in the tested conditions. A different genetic background between the cKp CH1157 and the MDR HS11286 strains could also explain the difference in the in vitro effect of Tle1. In vivo, different stressors could induce expression of T6SS and/or facilitate Tle1 activity as was observed for other Enterobacteriaceae45. Moreover, to understand the role of this effector in a heterologous system, the tle1 gene was cloned into pBAD33 plasmids to directly produce it either in periplasmic or in cytoplasmic compartments of E. coli DH5α cells. Tle1 periplasmic production in E. coli DH5α strain did not induce cell lysis but impaired membrane permeability as measured by IP staining, and gave rise to a small colony phenotype. Similarly, Liu et al. demonstrated the antibacterial effect of HS11286 Tle1 phospholipase in an analogous system of periplasmic production of this effector in BL21 E. coli cells30. T6SS expression in the carbapenem-resistant HS11286 strain is upregulated in the presence of β-lactam antibiotics30. The authors performed E. coli toxicity assays in presence of 50 µg/mL of ampicillin, which upregulates T6SS expression and could, in synergy with Tle1, enhance membrane permeability and thereby lead to efficient cell toxicity. The T6SS-2 cluster found in CH1157 slightly differ from the T6SS-1 since it does not encode essential T6SS core components (TssBC, Hcp). As investigated by Storey et al., there is a great diversity in T6SS cluster organization in K. pneumoniae genomes46. Other K. pneumoniae genomes also possess incomplete T6SS clusters, whose role remains to be defined40. The independent functioning of truncated T6SS clusters is still questionable: their activity could be dependent of the simultaneous presence of a complete cluster as already described for orphan genes38. The CH1157 T6SS-2 possesses an endopeptidase-encoding gene (Fig. 1c) and its putative protein (Tke) contains a N-terminal LysM peptidoglycan binding domain and a M23 putative catalytic site in the C-terminal part. No cognate immune protein was identified around the Tke potential effector, likely due to the lack of data in the immune protein database36. Bioinformatic analyses have shown that CH1157 Tke are mainly found in K. pneumoniae species and share specific domains of other T6SSs effectors (Type VI Amidase Effector (Tae) and Type VI Metallopeptidase Effector (Tpe))47. Moreover, an SLT transglycosylase domain (PF 01464.23) previously identified in Tke was shown to belong to Lyz-like superfamily (cl00222) and to share domains with 11th effector group in K. pneumoniae described by Li et al.36. T6SS targeted cells could therefore be affected in their capacity to survive in a competitive and stressful environment such as the intestinal tract.
Our results showed that T6SS-1 facilitates long-term establishment of K. pneumoniae CH1476 in the gastro-intestinal tract of animals previously treated with streptomycin. Analyses of microbiota α-diversity of mice feces harvested both pre- and post-treatment (DO versus D-3) show that streptomycin affects microbiota richness, with a decrease in facultative anaerobes (especially Bacteroides genus) (Fig. 4d) as previously reported48. The results obtained with this animal model correlate with the long-term gastrointestinal carriage of K. pneumoniae in patients treated with antibiotics, indicating that intestinal microbiota dysbiosis promotes K. pneumoniae implantation7. T6SS has been previously shown to contribute to the overall fitness of hvKp in vivo, and its importance in gastrointestinal colonization and translocation was reported in a murine model with strain NTUH-K2044 a short time point after challenge (2 and 6 days post-infection)42. Lethal effects associated with the expression of T6SS were also reported with the hvKp 52.145 strain in a G. mellonela larvae infection model46 and a murine pulmonary infection model29. In the latter study, trans-complementation of the isogenic Δpld1 mutant, deficient for the synthesis of the phospholipase effector, only partially restored the lethality phenotype. Similarly, trans-complementation of the tssB gene in our study partially restored the lack of colonization in mice, probably due to stoichiometry modifications of the molecular compounds necessary for T6SS machinery establishment.
T6SS-1 is necessary for the colonization of the uppermost part of the intestinal tract of mice i.e. the proximal intestine and ileal loop. Major differences in microbiota composition along the mice gastrointestinal tract could explain the disparities in colonization linked to the presence of potential K. pneumoniae competitors: Lactobacillaceae are predominant in the oxygen-rich small intestine, and strictly anaerobic Bacteroidaceae and Lachnospiraceae are enriched in the large intestine and fecal content of mice49. The role of K. pneumoniae T6SS within the upper GIT microbiota remains to be unraveled. Colonization gradient of K. pneumoniae along the intestinal tract of mice is correlated with differences in concentrations of T6SS-triggering molecules, as the concentration peak of bile salts is observed in the proximal ileum and progressively decreases distally50. In vitro, the influence of intestinal parameters on T6SS expression has already been described with intestinal pathogens: the V. cholerae T6SS is activated by mucins and microbiota-modified bile salt51, the S. Typhimurium T6SS is activated by bile salts26, and the enteroaggregative E. coli Sci-1 T6SS is responsive to iron starvation52. In hvKp, other environmental factors such as presence of anti-microbial peptides (HBD-3), high osmotic pressure and pH variations influence the expression of T6SS-encoding genes30,42,46. More recently, overexpression of two different clusters of T6SS in K. pneumoniae ST258 was correlated, in vitro and in vivo, with an excess of oxidant stress such as encountered during airway infection suggesting a role of T6SS in pulmonary pathogenesis33.
Antibacterial activity of T6SS is involved in bacterial warfare, even in complex environments such as plants, insects, and the gut microbiota of mammals53. The study of interactions between commensal microbes and gastrointestinal pathogens is a growing field, and in vivo T6SS-mediated bacterial competition has already been observed in Salmonella enterica Typhimurium, Burkholderia thailandensis, E. coli (EHEC), Shigella sonnei, Bacteroides fragilis and Vibrio cholerae54. However, it is clearly advised that long-term experiments should understand the real contribution of antibacterial weapons in shaping microbial communities54. In our study, we showed that T6SS-1 was required for intestinal persistence of cKp, suggesting that this system mediates antagonistic bacterial interactions that impair microbiota resilience. Indeed, at day 50 after infection, when the WT strain and T6SS-1 mutant were still present in equal amounts in the feces of mice, the composition of microbiota in the different groups of infection was heterogeneous. There was a higher resilience of microbiota in the CH1476-∆tssB/pSTAB group of co-housing mice than in the two other groups, suggesting that T6SS affects the ability of the microbiota to refill itself after breakdown. The Oscillospiraceae family was significantly increased in absence of K. pneumoniae T6SS-1 and could thus be a potential target of T6SS since this family is present in the intestinal microbiota of mice infected with CH1476-∆tssB/pSTAB at both D-3 and D+50 (Fig. 5b). As previously suggested, T6SS effectors could mediate the specific elimination of metabolic competitors54, thus facilitating the colonization process. For instance, Vibrio fischeri specifically targets competitors via TasL lipoprotein cell–cell attachment55. An impact of T6SS-1 effectors on eukaryotic cells cannot be totally excluded, although no Tle1 direct effect on host cells has yet been reported. However, in P. aeruginosa, other Tle phospholipases (PldA / Tle5A, PldB / Tle5B and TplE / Tle4) have been shown as capable of targeting eukaryotic cells (activating PI3K/Akt pathway and promoting autophagy, respectively)21,35.
Our approach allowed a better understanding of bacterial interactions in gut microbiota mediated by the T6SS-1 of cKp. There is a clear knowledge gap in understanding the T6SS function in the context of the holobiont and more research in this area is necessary, especially considering the diversity of T6SS effectors and the activities that may have. Indeed, the effect on other microbiota members (fungi) and host cells21 remains to be investigated46. T6SS eukaryotic-targeting effectors could enhance pathogen virulence by modulating host epithelial cell integrity or protecting themselves from innate immune response56. Overcoming colonization resistance by T6SS related to modulation of host response during infection, making T6SS an efficient virulence factor for pathogens25. Improved mechanistic understanding of commensal–pathogen interactions is a fundamental prerequisite for the design of prophylactic and therapeutic approaches for Klebsiella control, especially for multidrug resistant strains.
Materials and methods
Identification of T6SS clusters in Klebsiella genus
The list of genomes used in this study is presented in Supplementary Table S1a. (i) KL427 database. All fully sequenced Klebsiella genomes from NCBI RefSeq were downloaded and analyzed (ftp://ftp.ncbi.nih.gov/genomes/, accessed in April 2019). Species assignation was corrected using Kleborate and virulence score assignation according to the presence of well-described virulence factors57. The species was assigned with a strong level of confidence for 414 genomes, and considered weak for 10 (Supplementary Fig. S2a). Three strains were discarded due to wrongful genus assignation. Additional K. quasipneumoniae subsp similpneumoniae genomes were downloaded from NCBI on July 6th 2022 (Supplementary Table S1b), and verified with Kleborate for correct species assignation. (ii) KL65 database. This database reflects Klebsiella diversity in terms of species and STs and was manually curated based on the availability of reliable isolation data.
Identification of T6SS systems
MacSyFinder37, an open-source software that detects macromolecular systems, was used to identify T6SS systems in Klebsiella genomes. As input, it requires: (i) a proteome, (ii) a set of Hidden Markov Model (HMM) protein profiles, and (iii) a model describing the number of genetic components and their organization. (i) Definition of HMM profiles. The HMM protein profiles have been described elsewhere38. Profiles for TssA, TssB, TssC, TssD (Hcp), TssE, TssF, TssG, TssH (ClpV), TssI (VgrG), TssJ, TssK, TssL, TssM and EvpJ, were used. (ii) Model. To identify bona fide T6SS, in Klebsiella genomes, the model corresponding to T6SSi was applied38, which sequentially requires 3 to 13 minimum mandatory proteins (biologically essential components for a putative functional system), associated with the T6SS. The different cluster organizations as well as their respective prevalence are presented in Supplementary Fig. S1. The different models gave qualitatively very similar results (Supplementary Fig. S2b). Decreasing the number of proteins required for a system to be considered complete resulted in an increase in secondary T6SS loci but had little effect on the number of genomes coding at least one T6SS (Supplementary Fig. S2c). The presence of at least 8 different core proteins was considered for the estimation of the prevalence of T6SS clusters, as it is the greatest number of necessary core proteins required to detect at least 2 T6SS in Klebsiella strains without inducing false negative results (Supplementary Figs. S2b and c). Additionally, components should be encoded in a single locus and the maximum distance between consecutive elements limited to 20 proteins. Models were written in plain text using a specific XML grammar and can be modified by the user (see http://macsyfinder.readthedocs.io/en/latest/ for details). (iii) Identification of effectors. BLASTP (v BLAST 2.7.1+, default parameters) were performed to identify homologous Tle1-like proteins in genomes of KL65 database to Tle1 protein encoded in the first T6SS locus of CH1157. At least 80% of protein identity was required to consider Tle1-like presence. Similarly, the presence of Tke-like proteins was evaluated by BLASTP (v BLAST 2.7.1+, default parameters) using the sequence of the endopeptidase of CH1157 as query against the RefSeq database (April 2019). The latter comprises a total of 14,363 chromosomes and 11,806 plasmids, representing 13,514 complete genomes from 4232 bacterial species. At least 50% identity and a coverage of over 60% of the protein length (> 400) was required to consider Tke-like presence.
Klebsiella phylogeny (KL65 database)
(i) Identification of the persistent genome. To build the phylogeny, we first identified the persistent genome using PanACoTA58. The pan genome was inferred with the connected-component clustering algorithm of MMSeqs2 with pairwise bidirectional coverage > 0.8 and sequence identity > 0.8. The persistent genome was built from the pangenome, with a persistence threshold of 95%, meaning that a gene family had to be present in a single copy in at least 62 out of the 65 genomes to be considered persistent. Of the 22,288 gene families of the Klebsiella pangenome, 2969 were present in 95% of the genomes.
(ii) Construction of the phylogenetic tree. To compute the phylogenetic tree, families of the persistent genome were individually aligned with mafft (v7.407) (PMID: 23329690) from the protein sequences back-translated to DNA. The maximum likelihood tree was built using the nucleotide alignment of the persistent genome with IQ-TREE multicore (v2.1.2) under the GTR model with a total of 651,381 informative sites59. Information on strain collection regarding strains isolated from the hospital, community-acquired infections, the environment or carriage strains was reliable.
Bacterial strains, plasmids and culture conditions
Bacterial strains and plasmids used in this study are listed in Table 1. CH1476 corresponds to CH1157 strain deleted for ampicillin shv-1 B-lactamase resistance gene and replaced by aadA7 gene conferring streptomycin resistance to allow molecular biology and in vivo experiments39. Derived mutant strains (T6SS mutants) were constructed as described elsewhere using the Datsenko & Wanner method60. Unless otherwise indicated, bacteria were grown in lysogeny broth (LB) medium in glass tubes at 37 °C in a shaker (200 rpm), or on LB agar plates overnight at 37 °C. When required, antibiotics in media were added at the following concentrations: streptomycin (50 µg/mL), ampicillin (100 µg/mL), kanamycin (50 µg/mL), tetracycline (35 µg/mL), spectinomycin (50 µg/mL), chloramphenicol (35 µg/mL).
For trans-complementation assays, DNA fragments containing the entire tssB gene were amplified from K. pneumoniae CH1476 genomic DNA by an overlapping extension PCR using primers listed in Supplementary Table S2 (Ins-tssB-pSTAB-Fw and Ins-tssB-pSTAB-Rv). The resulting fragment was cloned into the linearized stable plasmid pSTAB61 previously amplified with the primers Lin-pSTAB-tssB-Fw and Lin-pSTAB-tssB-Rv (Supplementary Table S2) using Gibson Assembly Cloning Kit (NEW ENGLAND BIOLABS; Ipswich, Massachusetts, USA).
Plasmid vectors pBAD33-ssOmpA (periplasmic addressing of the product) and pBAD33-RBS (cytoplasmic addressing of the product) (Table 1) were used in cloning experiments62 performed in the Escherichia coli DH5α strain. The tle1 gene was amplified with high fidelity Phusion Taq (THERMOFISHER; Waltham, Massachusetts, USA) from CH1476 genomic DNA using oligonucleotides (Supplementary Table S2) containing SalI and HindIII restriction sites within their 5′ and 3′ ends respectively. The resulting tle1 amplification product was digested by SalI and HindIII restriction enzymes and then inserted into both pBAD33 plasmids (pBAD33-ssOmpA-tle1 and pBAD33-RBS-tle1) (Table 1). After electroporation, transformants were selected onto LB agar plates containing 0.8% glucose to inhibit “leaky expression” of the pBAD33 promoter and 35 µg/mL chloramphenicol. All constructions were verified by PCR (GoTaq Colorless Mastermix) (PROMEGA; Charbonnières les Bains, France) and by determination of DNA sequence (EUROFINS GENOMICS; Ebersberg, Germany).
Effect of tle1 expression in E. coli DH5α
Measurement of the Tle1 effect was assessed in E. coli DH5α pBAD33-derived strains (Table 1) by (i) bacterial growth monitoring and CFU counts, (ii) phenotypical analysis by agar plates spotting, and (iii) flow cytometry analysis after live-dead staining. (i) Briefly, overnight cultures in LB supplemented with 0.8% glucose were diluted to an OD620nm 0.001 in LB medium containing 0.2% arabinose and their growth was monitored for 8 h by OD620nm measurements. At the last time point, serial dilutions of the suspensions were plated onto LB agar supplemented with 0.8% glucose and chloramphenicol for CFU counting; (ii) O/N cultures adjusted to an OD620nm 0.2 were serially diluted, and 10µL of each dilution were spotted onto LB agar containing 0.2% arabinose and chloramphenicol; (iii) Cells from 8-h culture were harvested by centrifugation from batch culture (at 3800G), washed once with saline solution, and diluted 50-fold prior to live/dead staining. A standard nucleic staining protocol combining the cell-permeant fluorochrome SYBR Green I (SGI, THERMOFISHER) and the cell-impermeant Propidium Iodide (PI, SIGMA-ALDRICH; St-Quentin-Fallavier, France) was used to reveal live and dead cells. SG I (10,000 × stock) was added to the sample (1× final concentration) and incubated for 10 min before the addition of PI (10 µg/mL fc) for an additional 5 min, in the dark and at room temperature. Tubes containing stained cells were vortexed for 10 s before acquisition (flow rate 69.2μL/min, and at least 20,000 events collected) with a BD LSR Fortessa X20 flow cytometer (BD BIOSCIENCES; Le Pont de Claix, France). SGI and PI were excited by a 488 nm (50mW) laser and collected through a 530/30BP filter with a preceding 505LP filter for SGI and through a 610/20BP filter with a preceding 600LP filter for PI. Counts were obtained in a bivariate dot plot in which live and dead cell populations were divided according to SYBR emission (on the abscissa axis) and PI emission (on the ordinate axis).
In vivo gastrointestinal colonization assays
Eight-week-old specific pathogen-free C57BL-6 J male mice (CHARLES RIVER; Saint Germain Nuelles, France) were housed six per cage at animal biosafety level 2 (21–22 °C, 12 h/12 h light–dark cycle) with access to food and water ad libitum. All experiments were performed in compliance with ARRIVE guidelines and according to the ethical guidelines set out in the Guide for the Care and Use of Laboratory Animals and with approval of the “Comité d’Ethique pour l’Expérimentation Animale Auvergne” (C2E2A), the local ethics committee (Reference number: APAFIS#17520-2019030413278293). After + /− pre-treatment for 72 h with 5 g/L of streptomycin in drinking water, mice received 1 × 108 CFU equivalent doses of K. pneumoniae WT and derived strains in 200µL of PBS via the intragastric route. Feces were first collected daily, then weekly, weighed, and homogenized in PBS (500–1000 µL). Serial dilutions were plated onto selective LB agar media with an appropriate antibiotic (ampicillin 100 µg/mL) to evaluate K. pneumoniae colonization levels in mouse intestine (Fig. 3a). Results were expressed as Log10 of numbers of CFU/g of feces. Before antibiotic treatment (D-3), the day of infection (D0) and 50 days after inoculation (D50), feces were also collected and frozen at − 80 °C until nucleic acid extraction. At the end of the experiment, mice were killed by cervical dislocation in strict accordance with ethical recommendations. The contents of different gastrointestinal tract parts (proximal, ileal loop, caecum, colon) were collected, serially diluted in PBS and plated onto selective LB agar with an appropriate antibiotic (ampicillin 100 µg/mL) to assess pathogen distribution throughout the gastrointestinal tract. Results were expressed as Log10 of numbers of CFU/mg of content.
DNA extraction from feces and 16S sequencing
DNA was extracted from mice feces with the FastDNA Spin Kit for Soil (MPBIOMEDICALS) according to the manufacturer’s instructions. Total nucleic acid extracts were eluted in 50µL of DES Buffer. DNA content of each sample was assessed with Qubit (THERMOFISHER). Samples were stocked at − 20 °C until 16S DNA sequencing. V3 and V4 regions of 16S rRNA gene were amplified using PCR1F_460-PCR1R_460 primer pair (Supplementary Table S2). The amplicons were sequenced using Illumina Miseq (ILLUMINA INC; Waltham, San Diego, CA, USA) at the INRAE GeT platform (GenoToul, Castanet-Tolosan, France), generating 250 bp paired-end reads. Raw data were processed with the rANOMALY63 which relies on the dada2 R package to produce amplicon sequence variants (ASV) as taxonomic units. The resulting ASV table was filtered with a minimum relative abundance of 5 × 10–5 and used to perform statistical and diversity analyses.
Statistical analysis and data representation
Statistical analyses were performed with GraphPad Prism 9 (GraphPad Software; La Jolla, CA, USA). Amplicon sequencing data were explored with R version 3.5.3 and Explore Metabar, accessible from the Migale server (https://shiny.migale.inrae.fr/app/exploremetabar)63. Alpha diversity was explored with the Observed index for richness and the Shannon index for evenness. Pairwise comparison between time points were tested with TukeyHSD test and adjusted P-values were reported. Distances between samples (Beta diversity) were measured with unweighted unifrac distances and projected on a two dimensional spaces by Non-metric Multidimensional Scaling (NMDS). Group of samples are compared by a pairwise Adonis test. A P-value of less than 0.05 was considered as significant.
Data availability
The 16S rRNA gene sequences datasets generated and analyzed in this study are available in the NCBI repository with the BioProject ID: PRJEB52868 (https://www.ncbi.nlm.nih.gov/).
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Acknowledgements
We thank members of the PhD Committee of T.M: D. Boucher (M2iSH laboratory, UCA) and M. Meynier (NeuroDol laboratory, UCA), and E. Cascales (Laboratoires d’Ingénierie des Sytèmes Macromoléculaires, Aix-Marseille University) as well as P. Langella and G. Orianne (MICALIS, INRAe) for his expertise in the field. We also thank D. Jurenas for providing pBAD33 plasmids, N. Charbonnel for technical support in mice experiments and in molecular biology. We would like to thank H. Billard and J. Colombet, Plateforme CYSTEM – UCA PARTNER (Clermont-Ferrand, France), for their technical support and expertise. We would like to thank M. Boyer, C. Besnard and T. Fleury for their implication in the project during their internship. This work was supported by a fellowship from the Ministère de l’Enseignement Supérieur et de la Recherche et de l’Innovation awarded to T.M. S.M. acknowledges support received from the Agence Nationale de la Recherche (ANR) of the French government through the program Investissements d’Avenir (16-IDEX-0001 CAP 20-25), EMERGENCE 2018. O.R is funded by an ANR JCJC (Agence national de recherche) grant [ANR 18 CE12 0001 01 ENCAPSULATION].
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T.M., S.M., and C.F. conceived the project. T.M., S.M., C.F., S.B., and O.R., designed the experiments. T.M., S.M., S.B., S.T., and O.R. performed in silico analysis. T.M., S.M., L.N., performed in vitro and in vivo assays. T.M., S.M., C.F., S.B., and O.R. wrote the manuscript with contribution from all the authors.
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Merciecca, T., Bornes, S., Nakusi, L. et al. Role of Klebsiella pneumoniae Type VI secretion system (T6SS) in long-term gastrointestinal colonization. Sci Rep 12, 16968 (2022). https://doi.org/10.1038/s41598-022-21396-w
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DOI: https://doi.org/10.1038/s41598-022-21396-w
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