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Peripheral Th17/Treg imbalance in elderly patients with ischemic stroke

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A Correction to this article was published on 20 February 2018

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Abstract

CD4+CD25+ regulatory T (Treg) cells and Th17 cells play important roles in peripheral immunity. Immune responses are main elements in the pathogenesis of ischemic stroke (IS). The contribution of Th17 cells in IS patients has not been proved, and whether the balance of Treg/Th17 cells is changed in IS patients remains unidentified. In the present study, we studied Th17 and Treg cell frequency, cytokine secretion, expression of transcription factors, and microRNAs related to Th17 and Treg cells differentiation, which is compared between IS patients and control group. Thirty patients with IS and 30 individuals as control group were enrolled in this study. The frequency of Th17 and Treg lymphocytes, the expression of transcription factors and microRNAs related to these cells, and the serum levels of associated cytokines were assessed by flow cytometry, real-time PCR, and ELISA, respectively. A significant reduction in proportion of peripheral Treg cell frequency and the levels of TGF-β and FOXP3 expression were observed in patients with IS compared with controls, while the proportions of Th17 were increased dramatically, and these effects were along with increases in the levels of IL-17A and RORγt expression in IS patients. The levels of mir-326 and mir-106b-25 expression were increased in patients with IS. These studies suggest that the increase in proportion of Th17 cells and decrease in Treg cells might contribute to the pathogenesis of IS. Manipulating the balance between Tregs and Th17 cells might be helpful for the treatment of IS.

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Change history

  • 20 February 2018

    In the original article the terms RORγt and GF-β were misspelled throughout the text. They should read RORγt and TGF-β instead. We apologize for the inconvenience. The original article has been corrected.

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Acknowledgments

This work was financially supported by the grant of Aging Research Institute, Tabriz University of Medical Sciences, and Tabriz, Iran.

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Author notes

  1. Sanam Dolati and Majid Ahmadi contributed equally to this manuscript.

Authors and Affiliations

  1. Aging Research Institute, Tabriz University of Medical Sciences, Tabriz, Iran

    Sanam Dolati & Majid Ahmadi

  2. Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran

    Sanam Dolati, Majid Ahmadi, Homayoon Siahmansouri, Farhad Jadidi-Niaragh & Mehdi Yousefi

  3. Student’s Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran

    Sanam Dolati

  4. Department of Immunology, School of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran

    Sanam Dolati, Homayoon Siahmansouri, Zohreh Babaloo, Farhad Jadidi-Niaragh & Mehdi Yousefi

  5. Neurosciences Research Center, Tabriz University of Medical Sciences, Tabriz, Iran

    Mohammad Khalili & Ali Akabar Taheraghdam

  6. Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran

    Leili Aghebati-Maleki

  7. Pishtaz Teb Zaman Diagnostics, Tehran, Iran

    Vahid Younesi

  8. Department of Immunology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran

    Mehdi Yousefi

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  1. Sanam Dolati
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Correspondence to Mehdi Yousefi.

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The authors declare that there is no conflict of interests regarding the publication of this paper.

Additional information

The original version of this article was revised: The terms ROR훾t and TGF-훽 were misspelled throughout the text. They should read RORγt and TGF-β instead.

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Dolati, S., Ahmadi, M., Khalili, M. et al. Peripheral Th17/Treg imbalance in elderly patients with ischemic stroke. Neurol Sci 39, 647–654 (2018). https://doi.org/10.1007/s10072-018-3250-4

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