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Curcumin attenuates ischemia-like injury induced IL-1β elevation in brain microvascular endothelial cells via inhibiting MAPK pathways and nuclear factor-κB activation

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Abstract

Inflammatory reactions play a key role in the cerebral injury after stroke or other ischemic brain diseases. Curcumin, which is extracted from herb turmeric, has been reported to have anti-inflammatory effects. The present study was aimed to investigate the anti-inflammatory effects of curcumin on oxygen-glucose deprivation (OGD) injured brain microvascular endothelial cells (BMECs). Rat BMECs were used and the results showed that OGD induced a significant elevation of the leakage of lactate dehydrogenase and the secretion of the proinflammation cytokine, IL-1β. Activation of p38, JNK MAPKs, and NF-κB in BMECs was also observed after OGD. The treatment of curcumin (20 μM) inhibited the increased production of IL-1β both at the protein and mRNA levels. The increased phosphorylation of p38 and JNK induced by OGD was decreased under the treatment of curcumin, whereas the p38 inhibitor, SB203580, significantly inhibited OGD-induced IL-1β production, but the JNK inhibitor, SP600125, failed to do so. These results suggest that the inhibition of IL-1β by curcumin may dependent on the p38 signaling pathway. The OGD-induced IL-1β production was also inhibited by the NF-κB inhibitor, and curcumin suppressed OGD-induced NF-κB activation. Furthermore, the NF-κB activation was attenuated by the SB203580, indicating that NF-κB activation was dependent on p38 signaling pathway. The present study suggests that curcumin displays an anti-inflammatory effect on OGD-injured BMECs via down-regulating of MAPK and NF-κB signaling pathways and might have therapeutic potential for the ischemic brain diseases.

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Acknowledgments

This work was supported by the team of Scientific Research Innovation Foundation of Logistics University of Chinese People’s Armed Police Force (No.: WHTD201308-2). Curcumin attenuates ischemia-like injury induced IL-1β elevation in BMEC via inhibiting MAPK pathways and nuclear factor-κB activation.

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Authors and Affiliations

  1. Department of Human Anatomy, Tianjin Medical University, Tianjin, 300070, China

    Hua-jiang Dong

  2. Department of Anatomy and Histoembryology, Logistics University of Chinese People’s Armed Police Forces, Tianjin, 300162, China

    Hua-jiang Dong, Jian Xu, Peng-xiao Xu & Peng Wang

  3. Neurological Hospital, Affiliated Hospital of Logistics University of Chinese People’s Armed Police Forces, Tianjin, 300162, China

    Chong-zhi Shang & Ding-wei Peng

  4. Graduate Department of Logistics University of Chinese People’s Armed Police Forces, Tianjin, 300162, China

    Li Zhan

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  1. Hua-jiang Dong
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  2. Chong-zhi Shang
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  4. Jian Xu
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  5. Peng-xiao Xu
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  6. Li Zhan
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  7. Peng Wang
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Corresponding authors

Correspondence to Li Zhan or Peng Wang.

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Hua-jiang Dong, Chong-zhi Shang and Ding-wei Peng contributed equally to this work (co-first authors).

Li Zhan and Peng Wang are co-corresponding authors.

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Dong, Hj., Shang, Cz., Peng, Dw. et al. Curcumin attenuates ischemia-like injury induced IL-1β elevation in brain microvascular endothelial cells via inhibiting MAPK pathways and nuclear factor-κB activation. Neurol Sci 35, 1387–1392 (2014). https://doi.org/10.1007/s10072-014-1718-4

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  • DOI: https://doi.org/10.1007/s10072-014-1718-4

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