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The effect of lentivirus-mediated TH and GDNF genetic engineering mesenchymal stem cells on Parkinson’s disease rat model

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Abstract

This study was designed to assess the potential therapeutic efficacy of gene-modified mesenchymal stem cells (MSCs), MSCs-TH and MSCs-GDNF, in PD rats. Fifty-nine PD rat models were divided into five groups and then the gene-modified MSCs were transplanted into the striatum of rats according to the design. Apomorphine-induced rotational behavior in rats was observed weekly; rats which received both MSCs-TH and MSCs-GDNF showed the most significant improvement compared with those in other groups (P < 0.01). Three weeks later, immunohistochemistry analysis found TH-positive cells and GDNF-positive cells in striatal. Eight weeks later, PD rats were killed. HPLC and ELISA results showed DA and GDNF content in striatum of rats which received both MSCs-TH, and MSCs-GDNF was considerably higher compared with those of other groups (P < 0.01),respectively. In conclusion, our results suggest that combined transplantation of MSCs expressing TH and GDNF can lead to remarkable therapeutic effects in a rat model of PD.

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Acknowledgments

This study was supported by grants from the Natural Sciences Foundation of JiangSu Province (No.BK2007257).

Conflict of interest

The authors declare that they have no competing interests.

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Authors and Affiliations

  1. Department of Neurosurgery, Jiangsu Provincial Hospital of Traditional Chinese Medicine, 155 Hanzhong Road, 210029, Nanjing, People’s Republic of China

    Dezhi Shi & Juemin Gao

  2. Department of Neurosurgery, The First Affiliated Hospital of Nanjing Medical University, 300 Guangzhou Road, 210029, Nanjing, People’s Republic of China

    Gong Chen, Li Lv, Lixin Li, Dong Wei, Peiyuan Gu, Yi Miao & Weixing Hu

  3. Department of Surgery, The First Affiliated Hospital of Nanjing Medical University, 300 Guangzhou Road, 210029, Nanjing, People’s Republic of China

    Yi Miao

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  1. Dezhi Shi
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Correspondence to Yi Miao or Weixing Hu.

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Shi, D., Chen, G., Lv, L. et al. The effect of lentivirus-mediated TH and GDNF genetic engineering mesenchymal stem cells on Parkinson’s disease rat model. Neurol Sci 32, 41–51 (2011). https://doi.org/10.1007/s10072-010-0385-3

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  • DOI: https://doi.org/10.1007/s10072-010-0385-3

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