Skip to main content
SpringerLink
Log in

Construction of bioluminescent Lactobacillus casei CJNU 0588 for murine whole body imaging

  • Research Article
  • Published:
Food Science and Biotechnology Aims and scope Submit manuscript

Abstract

Recombinant DNA pLuc2 which contains a firefly luciferase cDNA from the commercial pGL3-Basic plasmid vector was constructed for bioluminescent Escherichia coli and lactobacilli. The bioluminescent signal strengths of E. coli DH5α and L. casei CJNU 0588 transformed with pLuc2 were 2.78×109 and 1.19×106 p/s, respectively. Approximately 1010 cells of bioluminescent L. casei CJNU 0588 (pLuc2) were given orally to 6-week-old female Balb/c mice. The bioluminescent signal was detected from all three mice at 1 h and one mouse at 3 h but was not detected thereafter. The signal was also confirmed from an opened abdomen and the extracted GI tract of a mouse at 1 h. From the results, we have known that L. casei CJNU 0588 pass through the murine stomach and reach the colon and cecum 1 h after oral administration.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Iebba V, Nicoletti M, Schippa S. Gut microbiota and immune system: An intimate partnership in health and disease. Int. J. Immunopath. Ph. 25: 823–833 (2012)

    CAS  Google Scholar 

  2. Robles Alonso V, Guarner F. Linking the gut microbiota to human health. Brit. J. Nutr. 109: S21–S26 (2013)

    Article  CAS  Google Scholar 

  3. Kanmani P, Satish Kumar R, Yuvaraj N, Paari KA, Pattukumar V, Arul V. Probiotics and its functionally valuable products-a review. Crit. Rev. Food Sci. 53: 641–658 (2013)

    Article  CAS  Google Scholar 

  4. Tsai YT, Cheng PC, Pan TM. The immunomodulatory effects of lactic acid bacteria for improving immune functions and benefits. Appl. Microbiol. Biot. 96: 853–862 (2012)

    Article  CAS  Google Scholar 

  5. de Moreno de LeBlanc A, Matar C, Perdigón G. The application of probiotics in cancer. Brit. J. Nutr. 98: S105–S110 (2007)

    Google Scholar 

  6. Katz JA. Probiotics for the prevention of antibiotic-associated diarrhea and Clostridium difficile diarrhea. J. Clin. Gastroenterol. 40: 249–255 (2006)

    Article  Google Scholar 

  7. Higashikawa F, Noda M, Awaya T, Nomura K, Oku H, Sugiyama M. Improvement of constipation and liver function by plant-derived lactic acid bacteria: A double-blind, randomized trial. Nutrition 26: 367–374 (2010)

    Article  CAS  Google Scholar 

  8. LeBlanc JG, Milani C, de Giori GS, Sesma F, van Sinderen D, Ventura M. Bacteria as vitamin supplier to their host: a gut microbiota perspective. Curr. Opin. Biotech. 24: 160–168 (2013)

    Article  CAS  Google Scholar 

  9. Pérez Martínez G, Bäuerl C, Collado MC. Understanding gut microbiota in elderly's health will enable intervention through probiotics. Benef. Microbes 5: 235–246 (2014)

    Article  Google Scholar 

  10. van Baarlen P, Wells JM, Kleerebezem M. Regulation of intestinal homeostasis and immunity with probiotic lactobacilli. Trends Immunol. 34: 208–215 (2013)

    Article  Google Scholar 

  11. Watson D, O’Connell Motherway M, Schoterman MHC, van Neerven RJ, Nauta A, van Sinderen D. Selective carbohydrates utilization by lactobacilli and bifidobacteria. J. Appl. Microbiol. 114: 1132–1146 (2013)

    Article  CAS  Google Scholar 

  12. Dunne C, O’Mahony L, Murphy L, Thornton G, Morrissey D, O’Halloran S, Feeney M, Flynn S, Fitzgerald G, Daly C, Kiely B, O'Sullivan GC, Shanahan F, Collins JK. In vitro selection criteria for probiotic bacteria of human origin: Correlation with in vivo findings. Am. J. Clin. Nutr. 73: 386S–392S (2001)

    CAS  Google Scholar 

  13. Rizzello V, Bonaccorsi I, Dongarrà ML, Fink LN, Ferlazzo G. Role of natural killer and dendritic cell crosstalk in immunomodulation by commensal bacteria probiotics. J. Biomed. Biotechnol. 2011: 473097 (2011)

    Article  Google Scholar 

  14. Cronin M, Sleator RD, Hill C, Fitzgerald GF, van Sinderen D. Development of a luciferase-based reporter system to monitor Bifidobacterium breve UCC2003 persistence in mice. BMC Microbiol. 8: 161 (2008)

    Article  Google Scholar 

  15. Loessner H, Leschner S, Endmann A, Westphal K, Wolf K, Kochruebe K, Miloud T, Altenbuchner J, Weiss S. Drug-inducible remote control of gene expression by probiotic Escherichia coli Nissle 1917 in intestine, tumor and gall bladder of mice. Microbes Infect. 11: 1097–1105 (2009)

    Article  CAS  Google Scholar 

  16. Lee MS, Moon GS. In vivo imaging of Escherichia coli and Lactococcus lactis in murine intestines using a reporter luciferase gene. Food Sci. Biotechnol. 21: 917–920 (2012)

    Article  CAS  Google Scholar 

  17. Daniel C, Poiret S, Dennin V, Boutillier D, Pot B. Bioluminescence imaging study of spatial and temporal persistence of Lactobacillus plantarum and Lactococcus lactis in living mice. Appl. Environ. Microb. 79: 1086–1094 (2013)

    Article  CAS  Google Scholar 

  18. Moon GS. Bifidobacterial growth stimulation by Lactobacillus casei via whey fermentation. J. Food Sci. Nutr. 14: 265–268 (2009)

    Article  CAS  Google Scholar 

  19. Sambrook J, Russell DW. Molecular cloning: A laboratory manual. 3rd ed. Cold Spring Harbor Laboratory Press, New York, NY, USA. pp. A3.2-3.3 (2001)

    Google Scholar 

  20. Park WJ, Lee KH, Lee JM, Lee HJ, Kim JH, Lee JH, Chang HC, Chung DK. Characterization of pC7 from Lactobacillus paraplantarum C7 derived from Kimchi and development of lactic acid bacteria-Escherichia coli shuttle vector. Plasmid 52: 84–88 (2004)

    Article  CAS  Google Scholar 

  21. Moon GS, Lee YD, Kim WJ. Screening of a novel lactobacilli replicon from plasmids of Lactobacillus reuteri KCTC 3678. Food Sci. Biotechnol. 17: 438–441 (2008)

    CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Biotechnology, Korea National University of Transportation, Jeungpyeong, Chungbuk, 368-701, Korea

    Ji-Eun Eom & Gi-Seong Moon

  2. Division of Bio-Imaging, Chuncheon Center, Korea Basic Science Institute, Chuncheon, Gangwon, 200-701, Korea

    Won-Gyeong Ahn & Song Her

Authors
  1. Ji-Eun Eom
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Won-Gyeong Ahn
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Song Her
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Gi-Seong Moon
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Gi-Seong Moon.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Eom, JE., Ahn, WG., Her, S. et al. Construction of bioluminescent Lactobacillus casei CJNU 0588 for murine whole body imaging. Food Sci Biotechnol 24, 595–599 (2015). https://doi.org/10.1007/s10068-015-0077-0

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10068-015-0077-0

Keywords

Search

Navigation